Rational Design of Intracellular Anticancer Drug Delivery: Nanocarriers Bearing Two-step Degradation Mechanism
| Project/Area Number |
24650279
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kobe University |
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Principal Investigator |
OOYA Tooru 神戸大学, 工学(系)研究科(研究院), 准教授 (10301201)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ナノキャリア / プロドラッグ / グルタチオントランスフェラーゼ / 抗がん剤 / 細胞内取り込み / デンドリマー / リポソーム / ミセル / ナノバイオ / 薬物送達システム / 細胞・組織 / ナノ材料 / 薬学 / 生体分子 |
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| Research Abstract |
A glutathione-p-nitroaniline conjugate was synthesized and examined binding of the conjugate with glutathione S-transferase (GST). When the conjugate was titrated with GST, absorbance increased with increasing concentration of GST, suggesting the binding the conjugate with GST. In addition, p-nitroaniline release was observed in the presence of GST. As for the design of nanocarriers for the prodrug, polyglycerol dendrimers (PGDs) were subjected and examined to clarify their interaction with the anti-cancer drug, 5-fluorouracil (5-Fu). The results of 19F-, 1H-NMR and fluorescence spectroscopy to confirm that PGD could encapsulate 5-Fu under aqueous conditions. Another examination of nanocarrier preparations, a liposome consisting of PGD-based phospholipids and a polymeric micelle consisting of vitamin E and a biocompatible polymer.
Through these studies, novel smart nanocarries in combination with the produg system toward GST targeting are feasible in near future.
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Report
(3 results)
Research Products
(23 results)
