| Project/Area Number |
24650325
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Nara Medical University |
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Principal Investigator |
TAKAKI Miyako 奈良県立医科大学, 医学部, その他 (00033358)
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| Co-Investigator(Renkei-kenkyūsha) |
KUNIYASU Hiroki 奈良県立医科大学, 医学部, 教授 (00253055)
NABEKURA Junichi 生理学研究所 (50237583)
NAKAI Junichi 埼玉大学 (80237198)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | リハビリテーション医学 / 5-HT4 受容体 / 腸壁内神経 / 神経細胞 / 再生 / 新生 / 吻合部 / トランスジェニックマウス / in vivoイメージング / 腸壁内神経系 / セロトニン４受容体 / 神経再生・新生 / ２光子顕微鏡 / H-lineマウス |
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| Research Abstract |
In the present study, using transgenic mice (TG) expressing GFP in neurons we succeeded in in vivo imaging of enteric neurons with 2 photon microscopy (2PM).
Then, the process of neurogenesis of injured enteric nervous system in TG mouse transected and re-astomosed gut was revealed by in vivo imaging with 2PM. We determined that a 5-HT4 receptor agonist facilitated to generate newborn enteric neurons at the anastomosis mediated via 5-HT4 receptor from neural stem cells. The neurogenesis of enteric neurons would give an improvement of dysfunction of gut function and defecation function.
This study is revolutionary and unexplored in medical field of rehabilitation.
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