Effects of immune cells on inflammatory responses and oxidative stress in the exercise-induced muscle damage

Research Project

Project/Area Number	24650410
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Sports science
Research Institution	Waseda University
Principal Investigator	SUZUKI Katsuhiko 早稲田大学, スポーツ科学学術院, 教授 (80344597)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	筋損傷 / 好中球 / 単球 / マクロファージ / 接着分子 / サイトカイン / MCP-1 / 炎症 / 活性酸素
Research Abstract	Although exercise induces muscle damage, the underlying mechanisms are not fully understand. The present study examined the involvement of neutrophils and macrophages in the exhaustive exercise-induced acute muscle damage in mice by application of the inhibitory neutrophil migration model and macrophage depletion model. The administlation of anti-neutrophil antibody inhibited the infiltration of Ly-6G positive cells (neutrophils) in the gastrocnemius, and the administration of clodronate liposome reduced the infiltration of F4/80 positive cells (macrophages) and related inflammatory reactions. Therefore, it was demonstrated that neutrophils and macrophages are involved in the exercise-induced muscle damage.