Application for amiono acids of tomato fruit.......

• Project/Area Number: 24650479
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Eating habits, studies on eating habits
• Research Institution: Kyushu University
• Principal Investigator: IWAYA-INOUE Mari 九州大学, (連合)農学研究科(研究院), 教授 (60091394)
• Co-Investigator(Kenkyū-buntansha): YUASA Takashi 宮崎大学, 農学部, 教授 (40312269)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: アミノ酸 / 栄養 / トマト / ダイズ / 果実

Research Abstract

Sequencing analysis of SlAlaRacemase1 and SlAlaRacemase2 for tomato fruit was determined. GST-fusion proteins for 45kDa 47kDa were also produced. In addition, semi-quantitative RT-PCR for tomato organs at the various developmental stages indicated that SlCIPK2 was expressed specifically in the reproductive organs. An anti-CIPK specific antibody recognized the recombinant SlCIPK2 specifically and cross-reacted to a CIPK-related polypeptide at a significant level in flower, particularly in stamen. The flower specific CIPK was tightly associated with the microsomes. In addition, expression profiles of branched chain amino acid transferase genes (BCATs) in soybean grains during pod filling stages were also indicated.

Research Products

• [Journal Article] Induction of a bZIP type transcription factor and amino acid catabolism-related genes in soybean seedling in response to starvation stress. (2013)
  • Author(s): Yuasa, T., Nagasawa, K., Osanai, A., Kaneko, A., Tajima, D., Nang, MPSH., Ishibashi, Y. and Iwaya-Inoue, M.
  • Journal Title: J. Bot. Volume: 2013 Pages: 1-8
  • DOI: 10.1155/2013/935479
  • Peer Reviewed
• [Journal Article] A flower specific calcineurin B-like molecule (CBL)-interacting protein kinase (CIPK) homolog in tomato cultivar Micro-Tom (Solanum lycopersicum L.) (2012)
  • Author(s): Yusasa, T., Yushi Ishibashi, Mari Iwaya-Inoue
  • Journal Title: Amer. J. Plant Sci Volume: 3 Pages: 753-763
• [Journal Article] A flower specific calcineurin B-likemolecule (CBL)-interacting protein kinase (CIPK) homolog in tomato cultivar Micro-Tom(Solanum lycopersicum L.) (2012)
  • Author(s): Yuasa T., Y. Ishibashi and M. Iwaya-Inoue
  • Journal Title: American Journal of Plant Sciences Volume: 3 Pages: 753-763
  • Peer Reviewed
• [Presentation] ダイズ子実の肥大成長に伴う分岐鎖アミノ酸アミノ基転移酵素(BCATs)遺伝子の発現変動 (2014)
  • Author(s): 長澤友里, 清水泰博, 長内克真, 金子彩実, 田島大地, 土居克美, 大島敏久, 石橋勇志, 井上眞理, 湯淺高志
  • Organizer: 日本作物学会
  • Place of Presentation: 千葉大学, 千葉市
  • Year and Date: 2014-03-29
• [Presentation] ダイズ子実の肥大成長に伴う分岐鎖アミノ酸アミノ基転移酵素(BCATs)遺伝子の発現変動 (2014)
  • Author(s): 長澤友里, 清水泰博, 長内克真, 金子 彩実, 田島大地, 土居克美, 大島敏久, 石橋勇志, 井上眞理, 湯淺高志
  • Organizer: 日本作物学会
  • Place of Presentation: 千葉大学、千葉市
• [Presentation] 糖飢餓シグナルに応答したダイズのbZIP型転写因子とアミノ酸転流関連遺伝子の発現誘導 (2013)
  • Author(s): 金子彩実,長 澤友里, 田島大地, 石橋勇志, 湯淺高志, 井上眞理
  • Organizer: 植物生理学会大会
  • Place of Presentation: 岡山大学, 岡山市
  • Year and Date: 2013-03-21
• [Presentation] 高等植物のオリゴ糖合成遺伝子発現による環境ストレス耐性獲得メカニズム (2013)
  • Author(s): 湯淺高志, 山口春香, 中村純也, 石橋勇志, 井上眞理
  • Organizer: 植物生理学会大会
  • Place of Presentation: 岡山大学, 岡山市
  • Year and Date: 2013-03-21
• [Remarks]
  • URL: http://www.agr.kyushu-u.ac.jp/lab/sakumotsu/