| Project/Area Number |
24650505
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | Kagawa Nutrition University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ラクターゼ / microRNA / 転写抑制 / 離乳期 / 発現変動 |
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| Outline of Final Research Achievements |
Lactase is an enzyme that is expressed in the small intestine of mammals. It hydrolyzes lactose, the only sugar present in maternal milk. There is high lactase activity during the lactation period, followed by a notable downregulation until the weaning period, but the mechanism behind this downregulation has yet to be clarified. The present study aimed to verify the hypothesis that a low molecular weight RNA (microRNA; miRNA) inhibits the transcription of the lactase gene. miRNAs were comprehensively analyzed using a 3D-Gene® miRNA Oligo chip, and the results showed the presence of multiple types of miRNAs that were expressed at a higher level from the weaning period onward than during the lactation period. However, it was not possible to confirm any complementarity between the candidate miRNAs and mRNAs for lactase or for transcription activating factors.
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