Reactivation of the inactive X chromosome through the asymmetric precursor cell division in the embryonal carcinoma cell population
| Project/Area Number |
24650614
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Tumor biology
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| Research Institution | Hokkaido University |
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Principal Investigator |
YOSHIDA Ikuya 北海道大学, 理学(系)研究科(研究院), 助教 (90240275)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 幹細胞 / 不活性X染色体 / 再活性化 / 非対称分裂 / 胚性腫瘍細胞 / 前駆細胞 / 国際情報交換 |
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| Research Abstract |
A single-cell derived cell populations of mouse embryonic stem (ES) and embryonal carcinoma (EC) cell are not homogeneous: heterogeneous cells in phenotype and/or function are frequently appeared in these undifferentiated cell populations. However mechanistic detail of their cellular heterogeneity remains unknown. We investigated how inactive X chromosomes (Xi) are reactivated in the undifferentiated EC cell population, and found that (1) stem-cell-like precursor cells are stochastically appeared in the populations, and (2) asymmetric cell divisions of the precursor cell generates a precursor cell and a HAT resistant (= Xi-reactivated) cell. Further analysis will be needed to clarify the underlying mechanisms for the acquisition of stem-cell-like properties, including Xi-reactivation ability, during asymmetric cell divisions.
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Report
(3 results)
Research Products
(3 results)
