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Development of in vivo screening systems for cancer metastasis suppressors

Research Project

Project/Area Number 24650627
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Tumor biology
Research Institution愛知県がんセンター(研究所)

Principal Investigator

AOKI MASAHIRO  愛知県がんセンター(研究所), 分子病態学部, 部長 (60362464)

Co-Investigator(Kenkyū-buntansha) SAKUMA Keiichiro  愛知県がんセンター(研究所), 分子病態 学部, 主任研究員 (90402891)
小島 康  愛知県がんセンター(研究所), 分子病態学部, 主任研究員 (30464217)
Co-Investigator(Renkei-kenkyūsha) KOJIMA Yasushi  愛知県がんセンター(研究所), 分子病態 学部, 主任研究員 (30464217)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords転移 / 大腸がん / shRNA / shRNAライブラリー
Research Abstract

Cancer metastasis is a complex process whose molecular mechanism is not fully understood. To identify targets for prevention and/or therapy of cancer metastasis, we developed a genome-wide shRNA library screen for colon cancer metastasis suppressor genes using an orthotopic transplantation mouse model. CMT93 cells, a murine colon cancer cell line with poor metastasizing activity, were transduced with lentiviral shRNA library and transplanted into the rectum of syngeneic C57/BL6 mice. The sequences encoding shRNA were retrieved from genomic DNA of the metastatic lesions by PCR for sequencing, followed by identification of the candidate genes targeted by the shRNA. Among 11 candidate genes identified so far, we focused on Hnrpll encoding an RNA-binding protein involved in pre-mRNA splicing, because the shRNA targeting the gene was identified from two metastatic lesions found in different transplanted mice. Knockdown of Hnrpll in CMT93 cells resulted in accelerated proliferation.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (21 results)

All 2013 2012 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (15 results) Remarks (1 results)

  • [Journal Article] Induction eof 6-sulfated glycans with cell adhesion activity via T-bet and GATA-3 in human helper T cells2012

    • Author(s)
      Sakuma K, Chen GY, Aoki M, Kannagi R
    • Journal Title

      Biochem. Biophys. Acta

      Volume: 1820 Pages: 841-848

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transcription factors c-Myc and CDX2 mediate E-selectin ligand expression in colon cancer cells undergoing EGF/bFGF-induced epithelial-mesenchymal transition2012

    • Author(s)
      Sakuma K, Aoki M, Kannagi R
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 109 Pages: 7776-7781

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sialic acid cyclization of human Th homing receptor glycan associated with recurrent exacerbations of atopic dermatitis2012

    • Author(s)
      Sakuma K, Aoki M, Kannagi R
    • Journal Title

      J. Dermatol. Sci

      Volume: 68 Pages: 187-193

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transcription factors c-MYC and CDX2 mediate E-selectin ligand expression in colon cancer cells undergoing EGF/bFGF-induced epithelial-mesenchymal transition2012

    • Author(s)
      Sakuma K, Aoki M, Kannagi R.
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 109(20) Issue: 20 Pages: 7776-7781

    • DOI

      10.1073/pnas.1111135109

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Induction of 6-sulfated glycans with cell adhesion activity via T-bet and GATA-3 in human helper T cells2012

    • Author(s)
      Sakuma K, Chen GY, Aoki M, Kannagi R.
    • Journal Title

      Biochim Biophys Acta

      Volume: 1820 Issue: 7 Pages: 841-848

    • DOI

      10.1016/j.bbagen.2012.03.005

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] mTORキナーゼ阻害薬は大腸がんモデルマウスの腺がん形成を強力に抑制する2013

    • Author(s)
      藤下晃章、武藤誠、青木正博
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      2013-12-05
    • Related Report
      2013 Final Research Report
  • [Presentation] 日本人胃がん患者の体組成変化2013

    • Author(s)
      小島康、松尾恵太郎、伊藤秀美、細野覚代、田中英夫、青木正博
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2013-10-05
    • Related Report
      2013 Final Research Report
  • [Presentation] 大腸がん細胞においてシアリルルイス糖鎖の発現とEMTは関連する2013

    • Author(s)
      佐久間圭一朗、神奈木玲児、青木正博
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      名古屋
    • Year and Date
      2013-10-05
    • Related Report
      2013 Final Research Report
  • [Presentation] mTORキナーゼ阻害薬は大腸がんマウスモデルの大腸腺がん形成を抑制する2013

    • Author(s)
      藤下晃章、武藤誠、青木正博
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2013-10-04
    • Related Report
      2013 Final Research Report
  • [Presentation] CDX転写因子の標的分子PLEKHG1の発現低下は大腸腫瘍形成を促進する2013

    • Author(s)
      青木正博、藤下晃章、武藤誠
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2013-10-03
    • Related Report
      2013 Final Research Report
  • [Presentation] CDX Transcription Factors Positively Regulate Expression of PLEKHG1 in Intestinal Epithelium2012

    • Author(s)
      青木正博、後藤嘉子、武藤誠
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      博多
    • Year and Date
      2012-12-14
    • Related Report
      2013 Final Research Report
  • [Presentation] CDX Transcription Factors Positively Regulate Expression of PLEKHG1 in Intestinal Epithelium2012

    • Author(s)
      青木正博、武藤誠
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Year and Date
      2012-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] c-MycとCDX2はEMTを起こした大腸がん細胞におけるE-セレクチンリガンド糖鎖の発現を媒介する2012

    • Author(s)
      佐久間圭一朗、神奈木玲児、青木正博
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Year and Date
      2012-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] 大腸がん細胞においてシアリルルイス糖鎖の発現とEMTは関連する

    • Author(s)
      佐久間圭一朗、神奈木玲児、青木正博
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      ウインク愛知(名古屋市中村区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] mTORキナーゼ阻害薬は大腸がんマウスモデルの大腸腺がん形成を抑制する

    • Author(s)
      藤下晃章、武藤誠、青木正博
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(横浜市西区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] CDX転写因子の標的分子PLEKHG1の発現低下は大腸腫瘍形成を促進する

    • Author(s)
      青木正博、藤下晃章、武藤誠
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(横浜市西区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] mTORキナーゼ阻害薬は大腸がんモデルマウスの腺がん形成を強力に抑制する

    • Author(s)
      藤下晃章、武藤誠、青木正博
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸国際展示場(神戸市中央区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] CDX転写因子は腸上皮細胞におけるPLEKHG1の発現を制御する

    • Author(s)
      青木正博
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      さっぽろ芸文館 (札幌)
    • Related Report
      2012 Research-status Report
  • [Presentation] CDX Transcription Factors Positively Regulate Expression of PLEKHG1 in Intestinal Epithelium

    • Author(s)
      青木正博
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      福岡国際会議場 (博多)
    • Related Report
      2012 Research-status Report
  • [Presentation] c-MycとCDX2はEMTを起こした大腸がん細胞におけるE-セレクチンリガンド糖鎖の発現を媒介する

    • Author(s)
      佐久間圭一朗
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      さっぽろ芸文館 (札幌)
    • Related Report
      2012 Research-status Report
  • [Remarks] 愛知県がんセンター研究所 部門紹介 分子病態学部

    • URL

      http://www.pref.aichi.jp/cancer-center/ri/01bumon/07bunshi_byotai/

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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