Development of tumor-targeted therapy using lentivirus-displayed antibody library
| Project/Area Number |
24650629
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Tumor immunology
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| Research Institution | Tottori University |
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Principal Investigator |
NAKAMURA Takafumi 鳥取大学, 医学(系)研究科(研究院), 准教授 (70432911)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 癌 / 抗体 / バイオテクノロジー / ウイルス / 生体機能利用 |
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| Research Abstract |
We previously showed that antibody-targeted cell fusion can be achieved by engineering a fusogenic viral membrane glycoprotein complex. Single-chain antibodies were displayed at the extracellular C terminus of the measles hemagglutinin (H) protein, and combinations of point mutations were introduced to ablate its ability to trigger fusion through the native viral receptors CD46 and SLAM. When coexpressed with the measles fusion (F) protein, the retargeted H proteins (Haals-scFvs) could mediate antibody-targeted cell fusion of receptor-negative or receptor-positive index cells with receptor-positive target cells. Now we have successfully pseudotyped lentiviral vectors with the glycoproteins Haals-scFv and F for targeted cell entry. Thus, the use of single chain antibodies for targeting potentially allows us to redirect the virus against any chosen cellular receptor. We also try to develop lentivirus-displayed scFvs library using the technology for identification of tumor-specific scFv.
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Report
(3 results)
Research Products
(29 results)
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[Journal Article] Coxsackievirus B3 Is an Oncolytic Virus with Immunostimulatory Properties that Is Active Against Lung Adenocarcinoma2012
Author(s)
Miyamoto S, Inoue H, Nakamura T, Yamada M, Sakamoto C, Urata Y, Okazaki T, Marumoto T, Takahashi A, Takayama K, Nakanishi Y, Shimizu H, Tani K
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Journal Title
Cancer Research
Volume: (掲載確定)(印刷中)
Issue: 10
Pages: 2609-2621
DOI
Related Report
Peer Reviewed
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