| Project/Area Number |
24650630
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Tumor immunology
|
|---|
| Research Institution | University of Toyama |
|---|
Principal Investigator |
KISHI HIROYUKI 富山大学, 大学院医学薬学研究部(医学), 准教授 (60186210)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MURAGUCHI Atsushi 富山大学, 大学院医学薬学研究部(医学), 教授 (20174287)
OZAWA Tatsuhiko 富山大学, 大学院医学薬学研究部(医学), 助教 (10432105)
KOBAYASHI Eiji 富山大学, 大学院医学薬学研究部(医学), 助教 (70459733)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KANEKO Shuichi 金沢大学, 大学院医薬保健学総合研究科, 教授 (60185923)
MIZUKOSHI Eishiro 金沢大学, 医学部附属病院, 講師 (90345611)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 細胞免疫 / 細胞チップ / 抗原特異的T細胞 / T細胞受容体 / がん特異的T細胞 |
|---|
| Research Abstract |
Cancer-specific cytotoxic T lymphocytes play important roles in cancer immunity. Previously we developed microwell-array chips that have an array of about 250,000 microwells in which only single lymphocytes can be accommodated. In this study, we showed that we could detect antigen-specific T lymphocytes by culturing single lymphocytes in each microwell, stimulating them with antigenic peptides, and detecting their cytokine-secretion on the chip. By retrieving the detected single T lymphocytes, we could amplify TCRalpha/beta cDNAs, insert them in a retrovirus vector with homologous recombination, and examine their antigen-specificity by expressing them on a TCR-deficient T cell line. The whole process can be accomplished within 10 days. The system may greatly contribute to the cancer immunotherapy in the future.
|
