Generation of monoclonal antibodies targeting induced cancer stem cells

• Project/Area Number: 24650636
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnosis
• Research Institution: Yokohama City University
• Principal Investigator: RYO Akihide 横浜市立大学, 医学研究科, 教授 (20363814)
• Project Period (FY): 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 細胞 / 再生医学 / 癌 / トランスレーショナルリサーチ / 抗体

Research Abstract

To obtain monoclonal antibodies specifically targeting cancer stem cells, we immunized rat with induced cancer stem cells (iCSC) followed by the production of hybridomas. By using iCSC and breast cancer cell lines, we selected two monoclon al colones that only recognize iCSC but not brest cancer cell line. Protein array analysis revealed that ABL1 could be a target of the monoclonal antibodies. When iCSCs were treated with an ABL inhibitor, imatinib, self-renewal property of iCSCs was inhibited.

Research Products

• [Journal Article] Induction of cells with cancer stem-cell properties from non-tumorigenic human mammary epithelial cells by defined reprogramming factors. (2013)
  • Author(s): Nishi M, Sakai Y, Akutsu H, Yoji Nagashima Y, Quinn G, Masui S, Kimura H, Perrem K, Umezawa A, Yamamoto N, Lee SW, Ryo A
  • Journal Title: Ｏｎｃｏｇｅｎｅ Volume: 33 Issue: 5 Pages: 643-652
  • DOI: 10.1038/onc.2012.614
  • Peer Reviewed
• [Presentation] Induction of cells with cancer stem-cell properties from human mammary epithelial cells (2012)
  • Author(s): 西 真由子、梁 明秀
  • Organizer: 第71回 日本癌学会学術総会
  • Place of Presentation: ロイトン札幌(北海道)
• [Presentation] Identification of differentially expressed genes in induced caner stem cells (2012)
  • Author(s): 坂井 祐介、西 真由子、梁 明秀
  • Organizer: 第71回 日本癌学会学術総会
  • Place of Presentation: ロイトン札幌(北海道)
• [Presentation] Production of monoclonal antibody targeting cancer stem cells (2012)
  • Author(s): 渡邊 夕紀子、西 真由子、梁 明秀
  • Organizer: 第71回 日本癌学会学術総会
  • Place of Presentation: ロイトン札幌(北海道)
• [Presentation] Induction of cells with cancer stem-cell properties from human mammary epithelial cells. (2012)
  • Author(s): 西 真由子
  • Organizer: 第71回 日本癌学会学術総会
  • Place of Presentation: ロイトン札幌（北海道）
• [Presentation] Identification of differentially expressed genes in induced caner stem cells. (2012)
  • Author(s): 坂井 祐介
  • Organizer: 第71回 日本癌学会学術総会
  • Place of Presentation: ロイトン札幌（北海道）
• [Presentation] Production of monoclonal antibody targeting cancer stem cells. (2012)
  • Author(s): 渡邊 夕紀子
  • Organizer: 第71回 日本癌学会学術総会
  • Place of Presentation: ロイトン札幌（北海道）
• [Patent(Industrial Property Rights)] 人工癌幹細胞の作製方法及びその分化誘導方法 (2012)
  • Inventor(s): 梁明秀、西真由子
  • Industrial Property Rights Holder: 公立大学法人 横浜市立大学
  • Filing Date: 2012-05-27
  • Overseas