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Generation of monoclonal antibodies targeting induced cancer stem cells

Research Project

Project/Area Number 24650636
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Tumor diagnosis
Research InstitutionYokohama City University

Principal Investigator

RYO Akihide  横浜市立大学, 医学研究科, 教授 (20363814)

Project Period (FY) 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords細胞 / 再生医学 / 癌 / トランスレーショナルリサーチ / 抗体
Research Abstract

To obtain monoclonal antibodies specifically targeting cancer stem cells, we immunized rat with induced cancer stem cells (iCSC) followed by the production of hybridomas. By using iCSC and breast cancer cell lines, we selected two monoclon al colones that only recognize iCSC but not brest cancer cell line. Protein array analysis revealed that ABL1 could be a target of the monoclonal antibodies. When iCSCs were treated with an ABL inhibitor, imatinib, self-renewal property of iCSCs was inhibited.

Report

(2 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (8 results)

All 2013 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (6 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Induction of cells with cancer stem-cell properties from non-tumorigenic human mammary epithelial cells by defined reprogramming factors.2013

    • Author(s)
      Nishi M, Sakai Y, Akutsu H, Yoji Nagashima Y, Quinn G, Masui S, Kimura H, Perrem K, Umezawa A, Yamamoto N, Lee SW, Ryo A
    • Journal Title

      Oncogene

      Volume: 33 Issue: 5 Pages: 643-652

    • DOI

      10.1038/onc.2012.614

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Induction of cells with cancer stem-cell properties from human mammary epithelial cells2012

    • Author(s)
      西 真由子、梁 明秀
    • Organizer
      第71回 日本癌学会学術総会
    • Place of Presentation
      ロイトン札幌(北海道)
    • Related Report
      2012 Final Research Report
  • [Presentation] Identification of differentially expressed genes in induced caner stem cells2012

    • Author(s)
      坂井 祐介、西 真由子、梁 明秀
    • Organizer
      第71回 日本癌学会学術総会
    • Place of Presentation
      ロイトン札幌(北海道)
    • Related Report
      2012 Final Research Report
  • [Presentation] Production of monoclonal antibody targeting cancer stem cells2012

    • Author(s)
      渡邊 夕紀子、西 真由子、梁 明秀
    • Organizer
      第71回 日本癌学会学術総会
    • Place of Presentation
      ロイトン札幌(北海道)
    • Related Report
      2012 Final Research Report
  • [Presentation] Induction of cells with cancer stem-cell properties from human mammary epithelial cells.2012

    • Author(s)
      西 真由子
    • Organizer
      第71回 日本癌学会学術総会
    • Place of Presentation
      ロイトン札幌(北海道)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Identification of differentially expressed genes in induced caner stem cells.2012

    • Author(s)
      坂井 祐介
    • Organizer
      第71回 日本癌学会学術総会
    • Place of Presentation
      ロイトン札幌(北海道)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Production of monoclonal antibody targeting cancer stem cells.2012

    • Author(s)
      渡邊 夕紀子
    • Organizer
      第71回 日本癌学会学術総会
    • Place of Presentation
      ロイトン札幌(北海道)
    • Related Report
      2012 Annual Research Report
  • [Patent(Industrial Property Rights)] 人工癌幹細胞の作製方法及びその分化誘導方法2012

    • Inventor(s)
      梁明秀、西真由子
    • Industrial Property Rights Holder
      公立大学法人 横浜市立大学
    • Filing Date
      2012-05-27
    • Related Report
      2012 Final Research Report
    • Overseas

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Published: 2013-05-31   Modified: 2019-07-29  

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