| Project/Area Number |
24650637
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Tumor diagnosis
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| Research Institution | Osaka City University |
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Principal Investigator |
SHIOTA Masayuki 大阪市立大学, 医学(系)研究科(研究院), 講師 (30381990)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 熱ショックタンパク質 / エキソソーム / プロテオーム解析 |
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| Research Abstract |
Recent research has reported that HSP72 released from tumor cells on surface of exosome. Therefore, we aimed to assess the NHq method, which we established method to isolate of HSP72-binding proteins, for purification of exosome and to explore for biomarker. HSP72-binding molecules isolated with NHq could not involve CD63 and CD9. These markers of exosome were detected in flow-through of NHq method. HSP72 was mainly detected in the supernatant of ultracentrifugation. HSP72 did not exist on surface of exosome against previous report. Therefore, we concluded that NHq method could not isolate exosome. Whereas, we tried to explore the biomarker of multiple myeloma using NHq method. As a result, we identified 3 proteins in sera of patients. One of these proteins might be a biomarker of multiple myeloma.
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