| Project/Area Number |
24650640
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Tumor diagnosis
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| Research Institution | National Center of Neurology and Psychiatry |
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Principal Investigator |
MASUTANI Mitsuko 独立行政法人国立がん研究センター, 研究所, 分野長 (60238904)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAMURA Takeji 神奈川工科大学, 工学部, 教授 (50342910)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ポリ(ADP-リボース) / PARP-1 / 代謝物 / リボシルアデノシン / リボシルイノシン / 血液 / 尿 / マウス |
|---|
| Research Abstract |
To identify a good biomarker for monitoring effectiveness of radiation therapy and chemotherapy, here we focused on the specific metabolites of poly(ADP-ribose) and evaluated their significance. We have established a measurement system for the metabolites of poly(ADP-ribose). The metabolism of poly(ADP-ribose) was analyzed in mouse bloodstream and urine. When poly(ADP-ribose) was intravenously injected into mice, poly(ADP-ribose) was rapidly degraded and the metabolites appeared in urine. We have further improved the pretreatment conditions of samples and optimized for LC-MS conditions for the measurement. After gamma-irradiation, the metabolites of poly(ADP-ribose) were detected in the mouse blood samples.
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