Search of mutant p53-specific target of cancer cells

• Project/Area Number: 24650642
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Clinical oncology
• Research Institution: Tohoku University
• Principal Investigator: ISHIOKA Chikashi 東北大学, 加齢医学研究所, 教授 (60241577)
• Co-Investigator(Renkei-kenkyūsha): KATO Shunsuke 東北大学, 加齢医学研究所, 准教授 (40312657)
• Research Collaborator: IMAI Hiroo 東北大学, 大学院医学系研究科, 大学院生
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 変異p53 / 合成致死

Research Abstract

This study aimed to find out a synthetic lethal/sickness gene for cancer cells expressing R175H mutant p53, a most frequent mutation in human cancer. A lentiviral shRNA library was transfected in SF126 cells expressing R175H mutant p53 and multiple candidate synthetic lethal/sickness genes against R175H mutant p53 were isolated. One of genes, ID1, truly introduced synthetic lethal/sickness in SF126 by a lentivirus-mediated knockdown by ID1 shRNA. In future, development of ID1 inhibitor might contribute a new era of anti-cancer drugs.

Research Products

• [Journal Article] High throughput RNAi screening identifies ID1 as a synthetic sick/lethal gene interacting with the common TP53 mutation R175H. (2014)
  • Author(s): Imai H, Kato S, Sakamoto Y, Kakudo Y, Shimodaira H, Ishioka C.
  • Journal Title: Oncol Rep. Volume: 31 Issue: 3 Pages: 1043-1050
  • DOI: 10.3892/or.2013.2953
  • Peer Reviewed
• [Journal Article] High throughput RNAi screening identifies ID1 as a synthetic sick/lethal gene interacting with the common TP53 mutation R175H. (2014)
  • Author(s): Imai H, Kato S, Sakamoto Y, Kakudo Y, Shimodaira H, Ishioka C.
  • Journal Title: Oncology Reports Volume: 31
  • Peer Reviewed
• [Presentation] High throughput RNAi screening of synthetic lethal genes interacting with the common TP53 mutation R175H (2012)
  • Author(s): Imai, H.,Kato, S.,Sakamoto, Y.,Takahashi, S.,Kakudo Y.,Shimodaira H.,Ishioka C.
  • Organizer: The 103rd Annual Meeting of American Association of Cancer Research
  • Place of Presentation: Chicago, IL, U.S.A.
  • Year and Date: 2012-04-02
• [Presentation] High throughput RNAi-screening of synthetic sick/lethal genes interacting with the common TP53 mutation R175H. (2012)
  • Author(s): Imai H, et al.
  • Organizer: The AACR Annual Meeting 2012
  • Place of Presentation: Chicago, USA