| Project/Area Number |
24651006
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental dynamic analysis
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KASUGA Ikuro 東京大学, 工学(系)研究科(研究院), 講師 (20431794)
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| Co-Investigator(Kenkyū-buntansha) |
KURISU Futoshi 東京大学, 大学院工学系研究科, 准教授 (30312979)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 安定同位体プローブ法 / 湖沼 / 藻類産生有機物 / 微生物ループ / Limnohabitans / DNA-SIP / FTMS |
|---|
| Research Abstract |
The structure of microbial loop in lake was evaluated by stable isotope probing. 13C-labelled algal organic matter prepared by cultivating algal pure culture with 13C-bicarbonate was added to lake water. DNA analysis in different density fractions demonstrated that microorganisms related to Limnohabitans predominantly responded to algal organic matter. In addition, flagellates and ciliates also assimilated 13C by preying on Limnohabitans-related bacteria. The close relationship between Limnohabitans-related bacteria and cyanobacteria was observed in the field survey.
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