Exploration of unknown microbial function through gene expression

• Project/Area Number: 24651018
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Environmental dynamic analysis
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: MORONO Yuki 独立行政法人海洋研究開発機構, 高知コア研究所, サブリーダー (30421845)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 海底下生命圏 / 遺伝子発現 / 機能遺伝子 / 遺伝子機能 / 海底下 / 未知微生物 / evoglow

Research Abstract

In this study, we applied Substrate Induced Gene EXpression (SIGEX) method to decipher unknown gene function of environmental microbes. We used drilled subseafloor sediment samples as DNA sources and established highly efficient SIGEX procedure. We could obtain many organo-halogen induced DNA fragments originating diverse microbial genome. Although there are several issue to be solved, this SIGEX approach can be useful technique to explore functions of unknown microbes in variety of environment.

Research Products

• [Journal Article] High frequency of phylogenetically diverse reductive dehalogenase-homologous genes in deep subseafloor sedimentary metagenomes (2014)
  • Author(s): Kawai, M., Futagami, T., Toyoda, A., Takaki, Y., Nishi, S., Hori, S., Arai, W., Tsubouchi, T., Morono, Y., Uchiyama, I., Ito, T., Fujiyama, A., Inagaki, F., and Takami, H.
  • Journal Title: Front. Microbiol Volume: 5
  • DOI: 10.3389/fmicb.2014.00080
  • Peer Reviewed
• [Journal Article] Hot-alkaline DNA extraction method for deep subseafloor archaeal communities. (2014)
  • Author(s): Morono, Y., Terada, T., Hoshino, T., and Inagaki, F.
  • Journal Title: Appl. Environ. Microbiol. Volume: 80 Issue: 6 Pages: 1985-1994
  • DOI: 10.1128/aem.04150-13
  • Peer Reviewed
• [Journal Article] An improved cell separation technique for marine subsurface sediments: Applications for high-throughput analysis using flow cytometry and cell sorting (2013)
  • Author(s): Morono, Y., Terada, T., Kallmeyer, J., and Inagaki, F.
  • Journal Title: Envion. Microbiol. Volume: 15 Issue: 10 Pages: 2841-2849
  • DOI: 10.1111/1462-2920.12153
  • Peer Reviewed
• [Journal Article] Distribution of dehalogenation activity and characterization of organohalide-responsive genes in marine sediments of the Nankai Trough subduction zone (2013)
  • Author(s): Futagami, T., Morono, Y., Terada, T., Kaksonen, A. H., and Inagaki, F.
  • Journal Title: Phil. Trans. R. Soc. B Volume: 368 Issue: 1616 Pages: 20120249-20120249
  • DOI: 10.1098/rstb.2012.0249
  • Peer Reviewed
• [Journal Article] 海洋環境における脱ハロゲン呼吸細菌 (2013)
  • Author(s): 二神泰基, 諸野祐樹，稲垣史生
  • Journal Title: 環境バイオテクノロジー学会誌 Volume: 13 Pages: 117-123
  • NAID: 40020320812
• [Journal Article] 海底下における微生物とその代謝・活性－未知の海底下生命圏を紐解くテクニックとその応用 (2013)
  • Author(s): 諸野祐樹
  • Journal Title: 日本極限環境生物学会誌 Volume: 12 Pages: 65-70
  • NAID: 40020070807
• [Presentation] Metabolic activity of subseafloor microbes in the South Pacific Gyre (2013)
  • Author(s): Morono, Y., Ito, M., Terada, T., and Inagaki, F.
  • Organizer: AGU Fall Meeting
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2013-12-11
• [Presentation] Technological challenges for the advanced study of deep subseafloor life (2013)
  • Author(s): Morono, Y., Ito, M., Terada, T., and Inagaki, F.
  • Organizer: International Astrobiology Workshop 2013
  • Place of Presentation: JAXA/ISAS, Sagamihara, Kanagawa, Japan
  • Year and Date: 2013-11-29
• [Presentation] 海底下環境における微生物生命圏とその生態に関する研究 (2013)
  • Author(s): 諸野祐樹
  • Organizer: 日本極限環境生物学会
  • Place of Presentation: 明治大学,川崎市.(奨励賞受賞講演)
  • Year and Date: 2013-10-27
• [Presentation] 強アルカリ条件を用いた海底下試料からの効率的核酸抽出法の確立とその評価 (2013)
  • Author(s): 諸野祐樹,寺田武志,星野辰彦,稲垣史生
  • Organizer: 第14回極限環境生物学会年会
  • Place of Presentation: 明治大学,川崎市
  • Year and Date: 2013-10-27
• [Presentation] Technological challenges for the advanced study of subseafloor life (2013)
  • Author(s): Morono, Y., Terada, T., and Inagaki, F.
  • Organizer: Goldschmidt 2013
  • Place of Presentation: Florence, Italy
  • Year and Date: 2013-08-26
  • Invited
• [Presentation] 海底下における微生物とその代謝・活性,未知の海底下生命圏を紐解くテクニックとその応用 (2013)
  • Author(s): 諸野祐樹
  • Organizer: 極限環境生物学会第14回シンポジウム
  • Place of Presentation: 東洋大学
  • Year and Date: 2013-06-15
  • Invited
• [Presentation] NanoSIMSおよびセルソーティングによる南太平洋環流域堆積物試料中の微生物代謝活性解析 (2013)
  • Author(s): 諸野祐樹,伊藤元雄,寺田武志,稲垣史生
  • Organizer: 日本地球惑星科学連合2013年大会
  • Place of Presentation: 幕張
• [Presentation] NanoSIMSおよびセルソーティングによる南太平洋環流域堆積物試料中の微生物代謝活性解析 (2013)
  • Author(s): 諸野祐樹， 伊藤元雄，寺田武志， 稲垣史生
  • Organizer: 日本地球惑星科学連合2013年大会
  • Place of Presentation: 幕張メッセ、千葉県
• [Presentation] 海底下堆積物からの細胞分離法の確立,およびフローサイトメトリーによる菌数カウントと選択的細胞濃縮 (2012)
  • Author(s): 諸野祐樹,寺田武志,稲垣史生
  • Organizer: 極限環境生物学会第13回年会
  • Place of Presentation: 日本大学,東京
  • Year and Date: 2012-12-01
• [Presentation] 微生物は一億年生きる?-最新の技術で探る海底下生命圏とその生存戦略- (2012)
  • Author(s): 諸野祐樹
  • Organizer: 酵素工学研究会第68回講演会
  • Place of Presentation: 東京大学
  • Year and Date: 2012-10-05
  • Invited
• [Presentation] Carbon and nitrogen assimilation in deep subseafloor microbial cells (2012)
  • Author(s): Morono Y., Terada T., Nishizawa M., Ito M., Hillion F., Takahata N., Sano Y., and Inagaki F.
  • Organizer: Microenergy 2012
  • Place of Presentation: Aarhus, Denmark
  • Year and Date: 2012-05-07
• [Presentation] Cell abundance, metabolic activity, and genetic functions of subseafloor microbes in the South Pacific Gyre (2012)
  • Author(s): Yuki Morono, Motoo Ito, and Fumio Inagaki
  • Organizer: IODP Expedition 329, 2nd post cruise meeting
  • Place of Presentation: Hawaii, USA
• [Presentation] Technological challenges for the advanced study of subseafloor life
  • Author(s): Morono, Y., Terada, T., and Inagaki, F
  • Organizer: Goldschmidt 2013
  • Place of Presentation: Firenze Fiera Congress and Exhibition Centre, Florence, Italy
  • Invited
• [Presentation] 生命の生息限界、代謝速度の限界を探る―海底下における微生物とその活性―
  • Author(s): 諸野祐樹
  • Organizer: 日本極限環境生物学会 第14回シンポジウム
  • Place of Presentation: 東洋大学、東京都
  • Invited
• [Presentation] 海底下環境における微生物生命圏とその生態に関する研究
  • Author(s): 諸野祐樹
  • Organizer: 日本極限環境生物学会、奨励賞受賞講演
  • Place of Presentation: 明治大学、川崎市、神奈川県
  • Invited
• [Presentation] 基質誘導型遺伝子発現解析による海底下未知生命機能遺伝子の発掘
  • Author(s): 寺田武志, 諸野祐樹, 稲垣史生
  • Organizer: 第29回日本微生物生態学会年会
  • Place of Presentation: 鹿児島大学、鹿児島県