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Occurence of antibiotic resistance genes in wastewater treatment pkant and discharg into river

Research Project

Project/Area Number 24651025
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Environmental impact assessment/Environmental policy
Research InstitutionKyoto University

Principal Investigator

MASARU Ihara  京都大学, 工学(系)研究科(研究院), 研究員 (70450202)

Co-Investigator(Renkei-kenkyūsha) 松田 知成   (50273488)
田中 宏明   (70344017)
山下 尚之   (90391614)
中田 典秀   (00391615)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords薬剤耐性遺伝子 / 次世代シーケンサー / 下水処理場 / 河川 / 群集解析 / 網羅的解析 / 下水
Research Abstract

I investigated the occurrence and fate of Antibiotic resistance genes (ARGs) in wastewater treatment plant(WWTP) by using next generation sequencer. WWTP influent, primary effluent, sedondary effluent, and final effluent were collected at WWTP A in Japan. As a result, 115 kinds of ARGs were detected in influent, which were reduced to 91 in secondary effluent. This result indicate that many kinds of ARGs are entered into WWTP, and some of them can be removed during biological treatment in WWTP. 95 kinds of ARGs were still detected in final effluent, which means ARGs may spread out from WWTP and enter into water environment. I also confirmed that kinds abd amount of ARGs were increased at downstream side of wastewater effluent in river basin. This result also indicates that ARGs may spread out from WWTP and enter into water environment.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (9 results)

All 2013 2012 Other

All Presentation (9 results)

  • [Presentation] 次世代シーケンサーによる微生物群集構造解析2013

    • Author(s)
      井原賢、李善太、堅川陽平、花本征也、佐久間亮輔、松田知成、山下尚之、田中宏明
    • Organizer
      CREST横断ミーテイング「病原微生物と持続可能な水利用」
    • Year and Date
      2013-10-22
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of microbial community in wastewater treatment plant by next generation sequencing2013

    • Author(s)
      Ihara M, Lee S, Katakawa Y, Yamashita N, Matsuda T, Tanaka H
    • Organizer
      17th IWA International Health Related Water Microbiology
    • Place of Presentation
      Santa Catarina, Brazil
    • Year and Date
      2013-09-17
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 下水中の微生物のメタゲノム解析および薬剤耐性遺伝子の検出2013

    • Author(s)
      Masaru Ihara, Suntae Lee, Yohei Katakawa, Naoyuki Yamashita, Tomonari Matsuda, Hiroaki Tanaka
    • Organizer
      第3回NGS現場の会
    • Place of Presentation
      神戸
    • Year and Date
      2013-09-04
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 次世代シーケンサーを用いた、下水処理場における薬剤耐性遺伝子の網羅的検出2013

    • Author(s)
      井原賢,堅川陽平,李善太,松田知成,山下尚之,田中宏明
    • Organizer
      第16回水環境学会シンポジウム2013
    • Related Report
      2013 Final Research Report
  • [Presentation] ANALYSIS OF MICROBIAL COMMUNITY IN WASTEWATER TREATMENT PLANT BY NEXT GENERATION SEQUENCING2013

    • Author(s)
      Masaru Ihara, Suntae Lee, Yohei Katakawa, Naoyuki Yamashita, Tomonari Matsuda, Hiroaki Tnaka
    • Organizer
      17th International Symposium on Health-Related Water Microbiology
    • Place of Presentation
      フロリアノポリス、ブラジル
    • Related Report
      2012 Research-status Report
  • [Presentation] Basic consideration of detection of pathogens using next generation sequencer2012

    • Author(s)
      Masaru Ihara, Tomonari Matsuda, Suntae Lee, Hiroaki Tanaka
    • Organizer
      4th IWA Young Water Professional Conference 2012
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] 次世代シーケンサーによる微生物群集構造解析

    • Author(s)
      井原賢、李善太、堅川陽平、花本征也、佐久間亮輔、松田知成、山下尚之、田中宏明
    • Organizer
      CREST横断ミーテイング「病原微生物と持続可能な水利用」
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] 次世代シーケンサーを用いた、下水処理場における薬剤耐性遺伝子の網羅的検出

    • Author(s)
      井原賢、堅川陽平、李善太、松田知成、山下尚之、田中宏明
    • Organizer
      第16回日本水環境学会シンポジウム
    • Place of Presentation
      沖縄
    • Related Report
      2013 Annual Research Report
  • [Presentation] Basic consideration of detection of pathogens using next generation sequencer

    • Author(s)
      Masaru Ihara, Tomonari Matsuda, Suntae Lee, Hiroaki Tnaka
    • Organizer
      4th IWA Young Water Professional Conference 2012
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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