Mechanism of the spinal deformity of fish born in heavy oil polluted seawater, and rescue with the new detoxification protein

• Project/Area Number: 24651044
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Kanazawa University
• Principal Investigator: HAYAKAWA Kazuichi 金沢大学, 薬学系, 教授 (40115267)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Nobuo 金沢大学, 環日本海域環境研究センター, 准教授 (60242476) ; OSHIMA Yuji 九州大学, 大学院農学研究院, 教授 (70176874)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 生物影響 / 骨代謝 / 胚発生 / 解毒タンパク質 / 有害化学物質 / 海洋生態 / 環境分析 / 水産学

Research Abstract

Using bioassay systems with fishes, we examined the mechanism of spinal deformity of fish by the heavy oil pollution. As a result, hydroxylated polycyclic aromatic hydrocarbons (OHPAH) provided causing death in early embryogenesis of the medaka, and OHPAH influenced the cell activities in fish scales. We suggested that the main substances of poison causing deformity of the fish are OHPAHs. Furthermore, we found the material which could become a candidate of the detoxification protein.

Research Products

• [Journal Article] Increased Prostaglandin E_2 has a positive correlation with plasma calcium during goldfish reproduction (2014)
  • Author(s): Suzuki, N., Maruyama, Y., Nakano, M., Hattori, A., Honda, M., Shimasaki, Y., Sekiguchi, T., Hishima, H., Wada, S., Srivastav, A.K., Hayakawa, K., Oshima, Y.
  • Journal Title: J. Fac. Agr., Kyushu Univ. Volume: 59 (1) Pages: 97-101
  • NAID: 120005411069
  • URL: http://www.agr.kyushu-u.ac.jp/english/journal/
  • Peer Reviewed
• [Journal Article] のと海洋ふれあいセンターが観測した沿岸水のpH低下について (2014)
  • Author(s): 鈴木元気、牧野史弥、早川和一、東出幸真、達克幸、坂井恵一
  • Journal Title: のと海洋ふれあいセンター研究報告 Volume: 19 Pages: 1-6
  • URL: http://notomarine.jp/rep-list.htm
  • Peer Reviewed
• [Journal Article] Polychlorinated biphenyl (118) activates osteoclasts and induces bone resorption in goldfish (2014)
  • Author(s): Yachiguchi, K., Matsumoto, N., Haga, Y., Suzuki, M., Matsumura, C., Tsurukawa, M., Okuno, T., Nakano, T., Kawabe, K., Kitamura, K., Toriba, A., Hayakawa, K., Chowdhury, V.S., Endo, M., Chiba, A., Sekiguchi, T., Nakano, M., Tabuchi, Y., Kondo, T., Wada, S., Mishima, H., Hattori, A., Suzuki, N.
  • Journal Title: Environmental Science and Pollution Research Volume: 印刷中
  • NAID: 120005253213
  • Peer Reviewed
• [Journal Article] Increased Prostaglandin E2 has a positive correlation with plasma calcium during goldfish reproduction (2014)
  • Author(s): Suzuki, N., Maruyama, Y., Nakano, M., Hattori, A., Honda, M., Shimasaki, Y., Sekiguchi, T., Hishima, H., Wada, S., Srivastav, A.K., Hayakawa, K., Oshima, Y.
  • Journal Title: Journal of the Faculty of Agriculture Kyushu University Volume: 59 Pages: 97-101
  • NAID: 120005435507
  • Peer Reviewed
• [Journal Article] Zebrafish scales respond differently to in vitro dynamic and static acceleration: analysis of interaction between osteoblasts and osteoclasts. (2013)
  • Author(s): Kitamura, K., Takahira, K., Inari, M., Satoh, Y., Hayakawa, K., Tabuchi, Y., Ohgai, K., Nishiuchi, T., Kondo, T., Mikuni-Takagaki, Y., Chen, W., Hattori, A. and Suzuki, N.
  • Journal Title: Comp. Biochem. Physiol. Part A Volume: 166 Issue: 1 Pages: 74-80
  • DOI: 10.1016/j.cbpa.2013.04.023
  • Peer Reviewed
• [Journal Article] Biological Effects of Polycyclic Aromatic Hydrocarbon Derivatives (2013)
  • Author(s): Bekki, K., Toriba, A., Tang, N., Kameda, T., Hayakawa, K.
  • Journal Title: Journal of UOEH Volume: 35 Issue: 1 Pages: 17-24
  • DOI: 10.7888/juoeh.35.17
  • NAID: 110009593197
  • ISSN: 0387-821X, 2187-2864
  • Peer Reviewed
• [Journal Article] Static and Dynamic Hypergravity Responses of Osteoblasts and Osteoclasts in Medaka Scales (2013)
  • Author(s): Yano S, et al. (14番目)
  • Journal Title: Zoological Science Volume: 30 Issue: 3 Pages: 217-223
  • DOI: 10.2108/zsj.30.217
  • Peer Reviewed
• [Journal Article] Polychlorinated biphenyl (118) activates osteoclasts and induces bone resorption in goldfish. (2012)
  • Author(s): Yachiguchi, K., Matsumoto, N., Haga, Y., Suzuki, M., Matsumura, C., Tsurukawa, M., Okuno, T., Nakano, T., Kawabe, K., Kitamura, K., et al.
  • Journal Title: Env. Sci. Poll. Res. Volume: 12 Issue: 10 Pages: 1347-1355
  • DOI: 10.1007/s11356-012-1347-5
  • NAID: 120005253213
  • Peer Reviewed / Open Access
• [Journal Article] Determination of Calcium Sensing Receptor in the Scales of Goldfish and Induction of Its mRNA Expression by Acceleration Loading (2012)
  • Author(s): Kakikawa, M., Yamamoto, T., Chowdhury, V.S., Satoh, Y., Kitamura, K., Sekiguchi, T., et al.
  • Journal Title: Biological Sciences in Space Volume: 26 Issue: 0 Pages: 26-31
  • DOI: 10.2187/bss.26.26
  • NAID: 130004450623
  • ISSN: 0914-9201, 1349-967X
  • Peer Reviewed
• [Journal Article] 魚類のウロコを用いた評価系の開発と骨代謝研究への応用 (2012)
  • Author(s): 鈴木信雄、舟橋久幸、耿啓達、柿川真紀子、山田外史、廣田憲之、北村敬一郎、清水宣明、早川和一、三島弘幸、岩坂正和、上野照剛、大森克徳、矢野幸子、池亀美華、田渕圭章、和田重人、近藤隆、服部淳彦
  • Journal Title: まぐね/Magune Jpn. Volume: 7 (4) Pages: 174-178
  • NAID: 10030958970
  • URL: http://www.magnetics.jp/journal/mag_2012/0704.html
  • Peer Reviewed
• [Journal Article] Prostaglandin E2 increases both osteoblastic and osteoclastic activities in the scales of goldfish and participates in the calcium metabolism in goldfish (2012)
  • Author(s): Omori, K., Wada, S., Maruyama, Y., Hattori, A., Kitamura, K., Sato, Y., Nara, M., Funahashi, H., Yachiguchi, K., Hayakawa, K., Endo, M., Kusakari, R., Yano, S., Srivastav, A.K., Kusui, T., Ejiri, S., Chen, W., Tabuchi, Y., Furusawa, Y., Kondo, T., Sasayama, Y. Nishiuchi, T., Nakano, M., Sakamoto, T. and Suzuki, N.
  • Journal Title: Zool. Sci Volume: 29 Issue: 8 Pages: 499-504
  • DOI: 10.2108/zsj.29.499
  • Peer Reviewed
• [Journal Article] Analysis of 8-hydroxy-2’-deoxyguanosine in human urine using hydrophilic interaction chromatography with tandem mass spectrometry (2012)
  • Author(s): C. Hosozumi
  • Journal Title: J. Chromatogr. B Volume: 893 - 894 Pages: 173-176
  • DOI: 10.1016/j.jchromb.2012.02.043
  • Peer Reviewed
• [Journal Article] Cloning of two members of the calcitonin-family receptors from stingray, Dasyatis akajei Possible physiological roles of the calcitonin family in osmoregulation (2012)
  • Author(s): Suzuki N, Sekiguchi T, Satake H, Kato K, Nishiyama Y, Takahashi H, et al
  • Journal Title: Gene Volume: 499 Issue: 2 Pages: 326-331
  • DOI: 10.1016/j.gene.2012.03.042
  • Peer Reviewed
• [Journal Article] 魚類のウロコを用いた評価系の開発と骨代謝研究への応用 (2012)
  • Author(s): 鈴木 信雄
  • Journal Title: まぐね Volume: 7 Pages: 174-178
  • NAID: 10030958970
  • Peer Reviewed
• [Presentation] 能登半島における過去8年間の多環芳香族炭化水素類及び粒子状物質の大気内変動 (2014)
  • Author(s): 袴田真理子、唐寧、亀田貴之、鳥羽陽、早川和一
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本大学
• [Presentation] 能登半島における過去8年間の多環芳香族炭化水素類及び粒子状物質の大気内変動 (2014)
  • Author(s): 袴田真理子，唐 寧，亀田貴之，鳥羽 陽，早川和一
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本大学（熊本県熊本市）
• [Presentation] 立山室堂の積雪層の陰イオンと多環芳香族炭化水素類の分析 (2013)
  • Author(s): 袴田真理子、鈴木元気、牧野史弥、立松路也、唐寧、鳥羽陽、青木一真、早川和一
  • Organizer: 第30回イオンクロマトグラフィー討論会
  • Place of Presentation: 株式会社豊田中央研究所
• [Presentation] 多環芳香族炭化水素類の魚類の骨代謝に対する作用 (2013)
  • Author(s): 表俊樹、川部季美、北村敬一郎、服部淳彦、田淵圭章、近藤隆、鳥羽陽、早川和一、鈴木信雄
  • Organizer: 第38回日本比較内分泌学会大会
  • Place of Presentation: 宮崎市民プラザ
• [Presentation] ポリ塩化ビフェニル(PCB118)は魚の骨代謝を攪乱する (2013)
  • Author(s): 谷内口孝治、関口俊男、羽賀雄紀、松村千里、鶴川正寛、中野武、北村敬一郎、鳥羽陽、早川和一、近藤隆、田渕圭章、和田重人、遠藤雅人、服部淳彦、鈴木信雄
  • Organizer: 第84回日本動物学会
  • Place of Presentation: 岡山大学
• [Presentation] Determination of nitropolycyclic aromatic hydrocarbons in water samples (2013)
  • Author(s): Chondo, Y., Nassar, H. F., Yoshida, Y., Li, Y., Kameda, T., Toriba, A., Hayakawa, K.
  • Organizer: China-Japan-Korea Symposium on Analytical Chemistry, 2013
  • Place of Presentation: Fukuoka, Kyushu University
• [Presentation] Atmospheric polycyclic aromatic hydrocarbons in Noto Peninshula (2013)
  • Author(s): Hakamata, M.
  • Organizer: Japan from 2004. China-Japan-Korea Symposium on Analytical Chemistry, 2013
  • Place of Presentation: Fukuoka, Kyushu University
• [Presentation] Development and application of a fish scale in vitro assay system (2013)
  • Author(s): Suzuki, N.
  • Organizer: 2nd Joint Meeting of the International Bone and Mineral Society and the Japanese Society for Bone and Mineral Research
  • Place of Presentation: Kobe convention center & Kobe portopia hotel
• [Presentation] 立山室堂の積雪層の陰イオンと多環芳香族炭化水素類の分析 (2013)
  • Author(s): 袴田真理子，鈴木元気，牧野史弥，立松路也，唐 寧，鳥羽 陽，青木一真，早川和一
  • Organizer: 第30回イオンクロマトグラフィー討論会
  • Place of Presentation: 株式会社豊田中央研究所（愛知県長久手市）
• [Presentation] 多環芳香族炭化水素類の魚類の骨代謝に対する作用 (2013)
  • Author(s): 表 俊樹，川部季美，北村敬一郎，服部淳彦，田渕圭章，近藤 隆，鳥羽 陽，早川和一，鈴木信雄
  • Organizer: 第38回日本比較内分泌学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎県宮崎市）
• [Presentation] ポリ塩化ビフェニル（PCB118）は魚の骨代謝を攪乱する (2013)
  • Author(s): 谷内口孝治，関口俊男，羽賀雄紀，松村千里，鶴川正寛，中野武，北村敬一郎，鳥羽 陽，早川和一，近藤 隆，田渕圭章，和田重人，遠藤雅人，服部淳彦，鈴木信雄
  • Organizer: 第84回日本動物学会
  • Place of Presentation: 岡山大学（岡山県岡山市）
• [Presentation] Determination of nitropolycyclic aromatic hydrocarbons in water samples (2013)
  • Author(s): Chondo, Y., Nassar, H. F., Yoshida, Y., Li, Y., Kameda, T., Toriba, A., Hayakawa, K.
  • Organizer: China-Japan-Korea Symposium on Analytical Chemistry, 2013
  • Place of Presentation: 九州大学（福岡県福岡市）
• [Presentation] Atmospheric polycyclic aromatic hydrocarbons in Noto Peninshula, Japan from 2004 (2013)
  • Author(s): Hakamata, M.,Hayakawa, K.
  • Organizer: China-Japan-Korea Symposium on Analytical Chemistry, 2013
  • Place of Presentation: 九州大学（福岡県福岡市）
• [Presentation] Development and application of a fish scale in vitro assay system (2013)
  • Author(s): Suzuki, N.
  • Organizer: Second Joint Meeting of the International Bone and Mineral Society, Japanese Society for Bone and Mineral Research
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Invited
• [Presentation] キンギョの再生ウロコにおける隆起線形成リズム (2012)
  • Author(s): 黒田美翔、舟橋久幸、鬼木弘明、宇都理佳、筒井和義、鈴木信雄、服部淳彦
  • Organizer: 第37回日本比較内分泌学会大会
  • Place of Presentation: 福井
• [Presentation] Polychlorinated biphenyl disrupts bone metabolism in goldfish (2012)
  • Author(s): Yachiguchi, K., Matsumoto, N., Haga, Y., Suzuki, M., Matsumura, C., Tsurukawa, M., Okuno, T., Nakano, T., Kawabe, K., Kitamura, K., Toriba, A., Hayakawa, K., Hattori, A., Suzuki, N.
  • Organizer: 7th International PCB Workshop
  • Place of Presentation: Palais des CongrÈs of Arcachon
• [Presentation] キンギョの再生ウロコにおける隆起線形成リズム (2012)
  • Author(s): 黒田 美翔
  • Organizer: 第37回日本比較内分泌学会大会
  • Place of Presentation: 福井大学（福井県）
• [Presentation] Polychlorinated biphenyl disrupts bone metabolism in goldfish (2012)
  • Author(s): Yachiguchi Koji
  • Organizer: 7th International PCB Workshop
  • Place of Presentation: Palais des Congrès of Arcachon(France)