| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
DNA checkpoint mechanism is activated when cells receive the DNA damage stress that arose from gamma-ray exposure. Not only the DNA damage responsive signaling cascade, DNA damage checkpoint could act as anti-tumour mechanism to protect us from cancer. Thus to detect which cells activate DNA checkpoint could help detect precancerous cells. Here we tackled to construct the module to detect checkpoint activation in live cells. We constructed the GFP-tagged peptide to express in the cells, and the module translocate outside the nucleus upon DNA replicative stress. Now we are modifying the module to make it more clear response so that we could apply to monitor checkpoint activated cells in live organism.
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