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Approaches to enhance a repair ability of UV induced DNA damage by promotion of DNA damage-recognition

Research Project

Project/Area Number 24651052
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Risk sciences of radiation/Chemicals
Research InstitutionNara Medical University

Principal Investigator

SUGIURA Shigeki  奈良県立医科大学, 医学部, 准教授 (40179130)

Co-Investigator(Kenkyū-buntansha) MORI Toshio  奈良県立医科大学, 医学部, 研究教授 (10115280)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords修復 / DNA 損傷 / シクロブタン 型 ダイマー / モノクローナル 抗体 / CPD
Research Abstract

Cyclobutane pyrimidine dimers (CPDs) is one of the major types of DNA damage induced by solar UV and is repaired exclusively by a nucleotide excision repair system (NER) in humans. The efficiency of NER mostly depends on helix-distortion of DNA lesion. Therefore, increasing the helix-distortion by CPDs may enhance NER efficiency. We cloned VH and VL genes of anti-CPDs antibody and constructed a single-chain Fv (ScFv) containing C-terminal SV40 nulear localization signal. The binding affinity of the scFv for CPDs was significantly lower than that of original IgG. Then we constructed a Fab containing C-terminal SV40 NLS. It effectively bound to CPDs.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (11 results)

All 2014 2013 2012

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (6 results)

  • [Journal Article] Quantitative and in situ detection of oxidatively generated DNA damage 8,5’-cyclo-2’-deoxyadenosine using an immunoassay with a novel monoclonal antibody2014

    • Author(s)
      T. Iwamoto, P.J. Brooks, T. Nishiwaki, K. Nishimura, N. Kobayashi, S. Sugiura and T. Mori.
    • Journal Title

      Photochem. Photobiol.

      Volume: xx Issue: 4 Pages: 829-836

    • DOI

      10.1111/php.12239

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] TNF-alpha stimulates efficient JC virus replication in neuroblastoma cells2014

    • Author(s)
      Nukuzuma, S. Nakamichi, K. Kameoka, M. Sugiura, S. Nukuzuma, C. Tasaki, T. Takegami, T.
    • Journal Title

      J Med Virol

      Volume: XX Issue: 12 Pages: 2026-2032

    • DOI

      10.1002/jmv.23886

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Suppressive effect oh PARP-1 inhibitor on JC virus replication in vitro2013

    • Author(s)
      Nukuzuma, S., Kameoka, M., Sugiura, S., Nakamichi, K., Nukuzuma, C., and Takegami, T.
    • Journal Title

      J. Med. Virol.

      Volume: 85 Pages: 132-137

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Suppressive effect of PARP-1 inhibitor on JC virus replication in vitro2013

    • Author(s)
      Nukuzuma, S., Kameoka, M., Sugiura, S., Nakamichi, K., Nukuzuma, C., Takegami, T.
    • Journal Title

      J Med Virol

      Volume: 85 Issue: 1 Pages: 132-137

    • DOI

      10.1002/jmv.23443

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Exogenious human immunodeficiency virus-1 protein, Tat, enhances replication of JC virus efficiently in neuroblastoma cell lines2012

    • Author(s)
      Nukuzuma, S., Kameoka, M., Sugiura, S., Nakamichi, K., Nukuzuma, C., Miyoshi, I., and Takegami, T.
    • Journal Title

      J Med Virol

      Volume: 84 Issue: 4 Pages: 555-561

    • DOI

      10.1002/jmv.23239

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Quantitative and in situ detection of oxidatively generated DNA damage 8,5'-Cyclo-2'deoxyadenosine using an immunoassay with a novel monoclonal antibody2014

    • Author(s)
      Iwamoto, T., Brooks, P. J., Kobayashi, N., Sugiura, S., and Mori, T.
    • Organizer
      International symposium on xeroderma pigmentosum and related diseases : Disorderes of DNA damage response-Bench to -bedside-
    • Place of Presentation
      Kobe
    • Related Report
      2013 Final Research Report
  • [Presentation] Quantitative and in situ detection of oxidatively generated DNA damage 8,5’- cyclo- 2’-deoxyadenosine using an immunoassay with a novel monoclonal antibody2014

    • Author(s)
      Iwamoto, T. Brooks, P. J. Kobayashi, N. Sugiura, S. and Mori, T.
    • Organizer
      nternational symposium on xeroderma pigmentosum and related diseases: Disorders of DNA damage response - Bench to bedside-
    • Place of Presentation
      Kobe
    • Related Report
      2013 Annual Research Report
  • [Presentation] PARP-1阻害剤のin vitroにおけるウイルス増殖抑制効果について2013

    • Author(s)
      奴久妻聡一、亀岡正典、杉浦重樹、中道一生、奴久妻智代子、田崎隆史、竹上勉
    • Organizer
      日本ウイルス学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] PARP-1 阻害剤の in vitro における JC ウイルス増殖抑制効果について2013

    • Author(s)
      奴久妻聡、亀岡正典、杉浦重樹、中道一生、奴久妻智代子、田崎隆史、竹上勉
    • Organizer
      日本ウイルス学会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] HIV-1 Tatによる神経芽細胞腫でのJCウイルス増殖促進2012

    • Author(s)
      奴久妻聡一、亀岡正典、杉浦重樹、中道一生、奴久妻智代子、竹上勉
    • Organizer
      日本ウイルス学会
    • Place of Presentation
      大阪
    • Related Report
      2013 Final Research Report
  • [Presentation] HIV-1 Tatによる神経芽細胞腫でのJCウイルス増殖促進2012

    • Author(s)
      奴久妻聡一、亀岡正典、杉浦重樹、中道一生、奴久妻智代子、竹上勉
    • Organizer
      日本ウイルス学会
    • Place of Presentation
      大阪国際会議場
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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