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Screening of epimutagens using DNA repair system

Research Project

Project/Area Number 24651063
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Risk sciences of radiation/Chemicals
Research InstitutionOsaka Prefecture University

Principal Investigator

YAGI Takashi  大阪府立大学, 理学(系)研究科(研究院), 教授 (80182301)

Co-Investigator(Kenkyū-buntansha) KAWANISHI Masanobu  大阪府立大学, 大学院理学系研究科, 准教授 (70332963)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsエピジェネティクス / エピミュータジェン / シトシンメチル化 / エピミュータゲン / メチル化 / MGMT / 環境化学物質
Research Abstract

Abnormality of epigenetic changes is a cause of carcinogenesis and teratogenesis in the hjgher animals. Epigenetic changes in traits accompany the change in cytosine methylation patterns of the promoter region of the responsible genes. Exogenous chemicals causing the epigenetic changes are called epimutagens. In this study, the assay methods to screen chemicals causing demethylation of 5-methylcytosine were tried to establish. We constructed the method in which the 5-azacytidine (5azaC)-induced demethylation can be detected by green fluorescence or formation of methyl nitrosourea (MNU)- resistant colonies using HeLaMR tumor cells lacking expression of O6-methylguanene-DNA methyltransferase (MGMT). Expression of MGMT mRNA and protein in the 5-azaC-induced MNU-resistant cells were confirmed. Using this method, epimutagens would be detected among various chemicals in the environment.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (5 results)

All 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (3 results)

  • [Journal Article] Adduct formation and repair, and translesion DNA synthesis across the adductsin human cells exposed to 3 nitrobenzanthrone2013

    • Author(s)
      M. Kawanishi, Y. Fujikawa, H. Ishii, H. Nishida, Y. Higashigaki, T. Kanno, T. Matsuda, T. Takamura Enya, T. Yagi
    • Journal Title

      Mutat. Res

      Volume: 753 (2) Pages: 93-100

    • Related Report
      2013 Final Research Report
  • [Journal Article] Adduct formation and repair, and translesion DNA synthesis across the adducts in human cells exposed to 3-nitrobenzanthrone2013

    • Author(s)
      M. Kawanishi, Y. Fujikawa, et al.
    • Journal Title

      Mutation Research

      Volume: 753巻 Issue: 2 Pages: 93-100

    • DOI

      10.1016/j.mrgentox.2013.03.005

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] HeLaMR細胞におけるシトシンメチル基転移酵素阻害剤による0^6-メチルグアニン修復活性の回復2013

    • Author(s)
      谷口美由紀、川西優喜、八木孝司
    • Organizer
      日本環境変異原学会第42回大会
    • Place of Presentation
      岡山市コンペンションセンター
    • Year and Date
      2013-11-29
    • Related Report
      2013 Final Research Report
  • [Presentation] HeLa MR細胞におけるシトシンメチル基転移酵素阻害剤によるO 6 -メチルグアニン修 復活性の回復2013

    • Author(s)
      谷口美由紀、川西優喜、八木孝司
    • Organizer
      日本環境変異原学会第42回大会
    • Place of Presentation
      岡山市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 紫外線照射下のフェナレノンが誘発するDNA損傷と突然変異の解析2012

    • Author(s)
      東垣由夏、川西優喜、高村岳樹、八木孝司
    • Organizer
      日本環境変異原学会第41回大会
    • Place of Presentation
      静岡市グランシップ
    • Year and Date
      2012-11-29
    • Related Report
      2013 Final Research Report 2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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