| Project/Area Number |
24651064
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAJIWARA Kagemasa 東海大学, 医学部, 講師 (00204397)
SAKABE Koh 東海大学, 医学部, 教授 (70162302)
OHTSUKA Masato 東海大学, 医学部, 准教授 (90372945)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | シックハウス症候群 / 疾患感受性遺伝子 / 疾患モデルマウス / 遺伝子操作マウス / PNPLA6 / 有機リン / 遺伝子発現 / PNPLA6 gene / esterase / ROSA26 / CRISPR / トランスジェニックマウス / 遺伝子改変 / ゲノム編集 / 神経障害エステラーゼ / モデルマウス / OPIDN / 化学物質過敏症 |
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| Outline of Final Research Achievements |
Sick building syndrome (SBS) is a set of several clinically recognizable symptoms reported by occupants of a building without a clear cause. Neuropathy target esterase (NTE) is a membrane bound serine esterase and its reaction with organophosphates (OPs) can lead to OP-induced delayed neuropathy (OPIDN) and nerve axon degeneration. We found that the enzymatic activity of NTE was significantly higher (P<0.0005) in SBS patients compared with controls. Thus, we constructed the transgenic(TG) mice with PNPLA6 gene encoding human NTE. Eight lines were established and each mouse from 8 lines showed higher activity for NTE than that in non transgenic mouse. The activity level was different in each tissue and the activity became higher in the old TG mice than that in the young TG mice. To elucidate the effect of OP for these mice, the experiments are now on going and the preliminary data showed harmful effects of OP for transgenic embryos. In addition, we try to detect the NTE-OP complex.
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