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Analysis for the function of the candidate gene of Sick House Syndrome and development of disease model mouse

Research Project

Project/Area Number 24651064
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Risk sciences of radiation/Chemicals
Research InstitutionTokai University

Principal Investigator

KIMURA Minoru  東海大学, 医学部, 教授 (10146706)

Co-Investigator(Kenkyū-buntansha) KAJIWARA Kagemasa  東海大学, 医学部, 講師 (00204397)
SAKABE Koh  東海大学, 医学部, 教授 (70162302)
OHTSUKA Masato  東海大学, 医学部, 准教授 (90372945)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsシックハウス症候群 / 疾患感受性遺伝子 / 疾患モデルマウス / 遺伝子操作マウス / PNPLA6 / 有機リン / 遺伝子発現 / PNPLA6 gene / esterase / ROSA26 / CRISPR / トランスジェニックマウス / 遺伝子改変 / ゲノム編集 / 神経障害エステラーゼ / モデルマウス / OPIDN / 化学物質過敏症
Outline of Final Research Achievements

Sick building syndrome (SBS) is a set of several clinically recognizable symptoms reported by occupants of a building without a clear cause. Neuropathy target esterase (NTE) is a membrane bound serine esterase and its reaction with organophosphates (OPs) can lead to OP-induced delayed neuropathy (OPIDN) and nerve axon degeneration. We found that the enzymatic activity of NTE was significantly higher (P<0.0005) in SBS patients compared with controls. Thus, we constructed the transgenic(TG) mice with PNPLA6 gene encoding human NTE. Eight lines were established and each mouse from 8 lines showed higher activity for NTE than that in non transgenic mouse. The activity level was different in each tissue and the activity became higher in the old TG mice than that in the young TG mice. To elucidate the effect of OP for these mice, the experiments are now on going and the preliminary data showed harmful effects of OP for transgenic embryos. In addition, we try to detect the NTE-OP complex.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (21 results)

All 2014 2013 2012

All Journal Article (10 results) (of which Peer Reviewed: 9 results,  Open Access: 1 results) Presentation (10 results) Book (1 results)

  • [Journal Article] Neuropathy Target Esterase (神経障害標的エステラーゼ)遺伝子導入マウスの作製2014

    • Author(s)
      加賀谷徹、割田貴之、三浦浩美、大塚正人、大久保朋一、坂部貢、木村穰
    • Journal Title

      臨床環境医学

      Volume: 23(1)

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Qualitative measurement of pain by analyzing the salivary alpha amylase.2014

    • Author(s)
      Tsuchiya, K., Saidin, MYB., Inoue, T., Kajiwara, K and Kimura, M.
    • Journal Title

      Precision Engineering

      Volume: 38 Pages: 257-260

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Interaction between carbon nanotubes and human cell.2014

    • Author(s)
      Tsuchiya, K., Iimori, S., Kajiwara, K. and Kimura, M.
    • Journal Title

      Precision Engineering

      Volume: 38 Pages: 116-120

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Improved loop-mediated isothermal amplification for HLA-DRB1 genotyping using RecA and a restriction enzyme for enhanced amplification specificity2013

    • Author(s)
      Mitsunaga S, Shimizu S, Okudaira Y, Oka A, Tanaka M, Kimura M, Kulski JK, Inoue I, Inoko H
    • Journal Title

      Immunogenet

      Volume: 65(6) Issue: 6 Pages: 405-415

    • DOI

      10.1007/s00251-013-0690-0

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Improvement of pronuclear injection-based targeted transgenesis (PITT) by iCre mRNA-mediated site-specific recombination2013

    • Author(s)
      Ohtsuka M, Miura H, Hayashi H, Nakaoka H, Kimura M, Sato M, Gurumurthy CB, Inoko H
    • Journal Title

      Transgenic Research

      Volume: 22 Issue: 4 Pages: 873-875

    • DOI

      10.1007/s11248-013-9703-x

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] X-ray microtomographic visualization of Escherichia coli by metalloprotein overexpression2013

    • Author(s)
      Mizutani, R., Taguchi, K., Ohtsuka, M., Kimura, M., Takeuchi, A., Uesugi, K. and Suzuki, Y
    • Journal Title

      Journal of Synchrotron Radiation

      Volume: 20 Pages: 581-586

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Improvement of pronuclear injection-based targeted transgenesis (PITT) by iCre mRNA-mediated site-specific recombination2013

    • Author(s)
      Ohtsuka, M., Miura, H., Hayashi, H., Nakaoka, H., Kimura, M.., Sato, M. and Gurumurthy , CB
    • Journal Title

      Transgenic Research

      Volume: 111 Pages: 111-222

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Association of sick building syndrome with neuropathy target esterase (NTE) activity in Japanese.2012

    • Author(s)
      Matsuzaka Y
    • Journal Title

      Environmental Toxicology

      Volume: 18 Issue: 10 Pages: 111-222

    • DOI

      10.1002/tox.21839

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fluorescent transgenic mice suitable for multi-color aggregation chimera studies2012

    • Author(s)
      Ohtsuka M, Miura H, Gurumurthy CB, Kimura M, Inoko H, Yoshimura S, Sato M
    • Journal Title

      Cell and Tissue Research

      Volume: 350 Issue: 2 Pages: 251-260

    • DOI

      10.1007/s00441-012-1470-0

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] PITT: Pronuclear Injection-Based Targeted Transgenesis, a Reliable Transgene Expression Method in Mice2012

    • Author(s)
      Ohtsuka, M., Miura, H., Sato, M., Kimura, M., Inoko H., Gurumurthy, CB.
    • Journal Title

      Exp Anim.

      Volume: 61 Pages: 489-502

    • NAID

      10031121853

    • Related Report
      2012 Research-status Report
  • [Presentation] EndoGalCとtargeted toxinの組み合わせを用いたCRISPR系でノックアウトされた遺伝子改変細胞濃縮法の開発2014

    • Author(s)
      渡部聡、桜井敬之、中村伸吾、梶原景正、木村穰、佐藤正宏
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] NTE(神経障害標的エステラーゼ)遺伝子発現マウスと有機リン代謝2014

    • Author(s)
      山村勇貴、本杉奈美、三浦浩美、大塚正人、大久保朋一、吉野美千代、笹川昇、坂部貢、木村穰
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 有機リンの培養細胞に及ぼす効果について(1)2013

    • Author(s)
      割田貴之、本杉奈美、田中正史、大久保朋一、坂部貢、木村穣
    • Organizer
      第22回日本臨床環境医学会学術集会
    • Place of Presentation
      東京(日本歯科大学東京短期大学)
    • Related Report
      2013 Research-status Report
  • [Presentation] Neuropathy target esterase(神経障害標的エステラーゼ)遺伝子導入マウスの作成2013

    • Author(s)
      加賀谷徹、割田貴之、三浦浩美、大塚正人、大久保朋一、坂部貢、木村穣
    • Organizer
      第22回日本臨床環境医学会学術集会
    • Place of Presentation
      東京(日本歯科大学東京短期大学)
    • Related Report
      2013 Research-status Report
  • [Presentation] 22q11.2欠失症候群で欠失するヒトDGCR2遺伝子のTGF-β/BMP シグナルとの相互作用2013

    • Author(s)
      梶原景正、渡邊聡、麥倉信一郎、木村穣
    • Organizer
      第36 回日本分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
  • [Presentation] マウス繊維芽細胞においてSez12はTGF-ß シグナルに関与している2013

    • Author(s)
      麥倉信一郎、渡邊聡、梶原景正、木村穣
    • Organizer
      第36 回日本分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
  • [Presentation] pnpla6遺伝子と有機リン感受性ートランスジェニックマウス作製と遺伝子導入細胞の性質ー2013

    • Author(s)
      加賀谷 徹,大塚 正人,三浦 浩美,大久保 朋一,田中 正史,割田 貴之,本杉 奈美,坂部 貢,梶原 景正,加藤 明, 吉川 枝里,木村 穣
    • Organizer
      第36 回日本分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
  • [Presentation] 雄性不妊を呈するヒト疾患モデルマウスでの造精機能回復の試み2013

    • Author(s)
      本杉 奈美,亀山 洋子,木村 穣
    • Organizer
      第36 回日本分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
  • [Presentation] 新規レクチンをコードするSez12はT細胞増殖シグナルを調節する2012

    • Author(s)
      麥倉信一郎、渡部聡、梶原景正、木村穣
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡市
    • Related Report
      2012 Research-status Report
  • [Presentation] 22q11.2欠失症候群で欠失するヒトDGCR2遺伝子のマウスホモログSez12遺伝子の軟骨細胞分化への影響2012

    • Author(s)
      梶原景正、麥倉信一郎、渡部聡、木村穣
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡市
    • Related Report
      2012 Research-status Report
  • [Book] シックハウス症候群マニュアル2013

    • Author(s)
      木村穣
    • Publisher
      東海大学出版会
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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