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Analysis of metabolic network in dechlorinating enrichment culture and construction of model system

Research Project

Project/Area Number 24651075
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Environmental technology/Environmental materials
Research InstitutionShizuoka University

Principal Investigator

FUTAMATA Hiroyuki  静岡大学, 工学(系)研究科(研究院), 教授 (50335105)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords環境浄化 / トリクロロエテン / バイオレメディエーション / 脱塩素化 / Dehalococcoides / Methanogen / 競合 / Bioremediation / Dechlorination / Methanogens / Competitive inhibition
Research Abstract

The mechanism of hydrogen interspecies transfer which enables to dechlorinate trichloroethene (TCE) effectively was characterized. Methane production was observed during TCE-dechlorination in the non-effective dechlorinating culture, while methane was produced rapidly after TCE-dechlorination in the effective dechlorinating culture. Molecular techniques revealed that aceticlastic methanogens competed with Dechlorinator, on the other hand, hydrogenotrophic methanogens did not. These results suggested that the activity of anaerobic acetate oxidizing bacteria play a key role for efficient dechlorination. We succeeded to isolate anaerobic acetate oxidizing bacteria, closely related to Moraxella osloensis.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (17 results)

All 2014 2013 2012 Other

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (12 results) Remarks (2 results)

  • [Journal Article] Dynamics of Different Bacterial Communities Are Capable of Generating Sustainable Electricity from Microbial Fuel Cells with Organic Waste2014

    • Author(s)
      Shuji Yamamoto, Kei Suzuki, Yoko Araki, Hiroki Mochihara, Tetsuya Hosokawa, Yusuke Chiba, Hiroko Kubota, Owen Rubaba, Yosuke Tashiro, Hiroyuki Futamata
    • Journal Title

      Microbes and Environments

      Volume: 29 Issue: 2 Pages: 145-153

    • DOI

      10.1264/jsme2.ME13140

    • NAID

      130004057176

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Challenges for Complex Microbial Ecosystems: Combination of Experimental Approaches with Mathematical Modeling2013

    • Author(s)
      Haruta, S., T. Yoshida, Y. Aoi, K. Kaneko and H. Futamata
    • Journal Title

      Microbes and Environments

      Volume: 28 Issue: 3 Pages: 285-294

    • DOI

      10.1264/jsme2.ME13034

    • NAID

      10031191411

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 塩素化エテン類脱塩素化集積物における微生物群集構造の解析と脱塩素化能力2012

    • Author(s)
      森岡啓幸、佐藤成晃、判治輝章、二又裕之
    • Journal Title

      第18回地下水・土壌汚染とその防止対策に関する研究集会

      Volume: (CD-ROM)paper No.107

    • Related Report
      2013 Final Research Report
  • [Presentation] Electronic flow for efficient reductive dechlorination of trichloroethene in an enrichment culture2014

    • Author(s)
      Kenji Suzuki, Hiroyuki Morioka, Naruaki Sato, Teruaki Hanji, Yosuke Tashiro, and Hiroyuki Futamata
    • Organizer
      UPM-Shizuoka University International colloquium 2014
    • Place of Presentation
      Malaysia (Universiti Putra Malaysia)
    • Year and Date
      2014-03-03
    • Related Report
      2013 Final Research Report
  • [Presentation] 3. Electronic flow for efficient reductive dechlorination of trichloroethene in an enrichment culture.2014

    • Author(s)
      Kenji Suzuki, Hiroyuki Morioka, Naruaki Sato, Teruaki Hanji, Yosuke Tashiro, and Hiroyuki Futamata
    • Organizer
      UPM-Shizuoka University International colloquium 2014
    • Place of Presentation
      Universiti Putra Malaysia (Malaysia)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 効果的脱塩素化を可能とする微生物生態系における種間水素伝達系の特性解明2013

    • Author(s)
      鈴木健司、森岡啓幸、二又裕之
    • Organizer
      第44回中部化学関係学協会支部連合秋季大会
    • Place of Presentation
      静岡大学(静岡県浜松市)
    • Year and Date
      2013-11-02
    • Related Report
      2013 Final Research Report
  • [Presentation] 効果的脱塩素化を可能とする微生物生態系における種間水素伝達系の特性解明2013

    • Author(s)
      鈴木健司、森岡啓幸、二又裕之
    • Organizer
      第44回中部化学関係学協会支部連合秋季大会
    • Place of Presentation
      静岡大学(静岡県浜松市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 効果的脱塩素化を可能とする微生物群集構造における種間水素伝達2012

    • Author(s)
      森岡啓幸、佐藤成晃、判治輝章、上田紘也、山本脩二、鷺坂智明、二又裕之
    • Organizer
      第64回日本生物工学会
    • Place of Presentation
      兵庫県神戸市(神戸アイランド)
    • Year and Date
      2012-10-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Construction of ortho-chlorinated toluene-dechlorinating enrichment culture2012

    • Author(s)
      Tomoaki Sagisaka, Hiroyuki Morioka, Kenneth H. Nealson, Hiroyuki Futamata
    • Organizer
      第28回日本微生物生態学会
    • Place of Presentation
      愛知県豊橋市(豊橋技術科学大学)
    • Year and Date
      2012-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Electron flow for efficient reductive dechlorination of trichloroethene in enrichment culture2012

    • Author(s)
      Hiroyuki Morioka, Naruaki Sato, Teruaki Hanji, Hiroya Ueda, Shuji Yamamoto, and Hiroyuki Futamata
    • Organizer
      WET2012 (Water and Envrionment Technology Conference in 2012)
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2012-06-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Electron flow for efficient reductive dechlorination of trichloroethene in enrichment culture2012

    • Author(s)
      Hiroyuki Morioka, Naruaki Sato, Teruaki Hanji, Hiroya Ueda, Shuji Yamamoto, and Hiroyuki Futamata
    • Organizer
      WET2012 (Water and Envrionment Technology Conference in 2012)
    • Place of Presentation
      Tokyo (Tokyo University)
    • Year and Date
      2012-06-30
    • Related Report
      2013 Final Research Report
  • [Presentation] 塩素化エテン類脱塩素化集積物における微生物群集構造の解析と脱塩素化能力2012

    • Author(s)
      森岡啓幸、佐藤成晃、判治輝章、二又裕之
    • Organizer
      第18回土壌地下水浄化研究集会
    • Place of Presentation
      埼玉県さいたま市
    • Year and Date
      2012-06-14
    • Related Report
      2013 Final Research Report
  • [Presentation] 塩素化エテン類脱塩素化集積物における微生物群集構造の解析と脱塩素化能力

    • Author(s)
      森岡啓幸
    • Organizer
      第18回土壌地下水浄化研究集会
    • Place of Presentation
      埼玉会館(埼玉県さいたま市)
    • Related Report
      2012 Research-status Report
  • [Presentation] Electron flow for efficient reductive dechlorination of trichloroethene in enrichment culture.

    • Author(s)
      Hiroyuki Morioka
    • Organizer
      WET2012 (Water and Envrionment Technology Conference in 2012)
    • Place of Presentation
      東京大学 (東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] 効果的脱塩素化を可能とする微生物群集構造における種間水素伝達

    • Author(s)
      森岡啓幸
    • Organizer
      日本生物工学会
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://cheme.eng.shizuoka.ac.jp/wordpress/futamatalab/member/hiroyuki-futamata/

    • Related Report
      2013 Final Research Report
  • [Remarks] 環境微生物生態工学研究室 静岡大学大学院工学研究科化学バイオ工学専攻 二又・田代研究室

    • URL

      http://cheme.eng.shizuoka.ac.jp/wordpress/futamatalab/

    • Related Report
      2013 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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