| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
To clarify the molecular mechanisms of uptake and high accumulation to PCB residues by Solidago canadenisis and Phytolacca americana L., it was attempted to use glycolipids obtained from these plants for assays of PCB congeners in the transgenic Arabidopsis plants carrying a reconbinant guinea pig AhR-mediated GUS reporter gene expression system. The glycolipids SG from the roots and DGDG from the leaves of S. canadenisis, and triterpenoidal glycosides (phytolalaccasapnins) from the leaves of P. americana L. increased PCB126-induced GUS activity. In contrast, SG from soy beads and DGDG from wheat did not affect PCB126-induced GUS activity. These results suggested glycolipids SG of S. canadenisis appeared to increase the uptake of PCB126, and glycolipids DGDG appeared to transport PCB126 into aerial parts. Moreover the present study suggested that possible to increase the sensitivity of the monitoring by adding these glycolipids.
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