Electron micrograph imaging of a single protein without staining
| Project/Area Number |
24651118
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Nanostructural science
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| Research Institution | Hosei University (2013)
University of Hyogo (2012) |
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Principal Investigator |
KIMURA Keisaku 法政大学, マイクロ・ナノテクノロジー研究センター, 研究員 (70106160)
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| Co-Investigator(Kenkyū-buntansha) |
YAO Hiroshi 兵庫県立大学, 物質理学研究科, 准教授 (20261282)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 無染色画像 / 電子顕微鏡基板 / グラフェンオキシド / 単分子タンパク質 / 透過走査電子顕微鏡 |
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| Research Abstract |
The object of this research is an imaging of a single protein molecule by a scanning transmission electron microscope (STEM) without staining. The key technology of this research is using a graphene oxide (GO) film as a substrate for STEM observation. The target resolution is 1 nm in the level of lysozyme protein. The outcome of the project were
1) Development of a single layer GO film on a Cu microgrid. 2) Preparation of protein sample on a GO single layer under low acceleration voltage without loss of resolution. 3) STEM monitoring of a single NADH ubiquinone reductase whose size is 20 nm without uranyl staining on a GO film.
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Report
(3 results)
Research Products
(4 results)
