Development of silica-biomineralizing peptides hinted by diatom frustule formation.
| Project/Area Number |
24651119
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nanostructural science
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| Research Institution | Kwansei Gakuin University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KANEKO Tadaaki 関西学院大学, 理工学部, 教授 (50291977)
OSAKI Koichi 関西学院大学, 理工学部, 教授 (40353320)
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| Research Collaborator |
INOUE Takayasu
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 自己組織化 / ナノバイオ / シリカ構造 / 塩基性ペプチド / 珪藻殻 |
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| Outline of Final Research Achievements |
The highly self-organized silica nano-structure in the diatom frustule is made partially by the function of basic polypeptides which are excreted to the frustule. In this study, we constructed artificial polypeptides resembling the diatom frustule factors, and characterized their functions. As a result, we succeeded to produce polypeptides with introduced basic amino acid residues such as Arg and Lys. All these newly made polypeptides showed a strong silica biomineralization activity, and the spherical diameter of mineralized silica could be controlled by the regulation of reaction conditions. By the addition of a specific antibody against these polypeptides to the reaction mixture, we also succeeded to make a plate-type biosilica. On the other hand, we made prototype SiC-based substrates and attempted to model a packing structure in the constrained reaction area.
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Report
(4 results)
Research Products
(25 results)
