3D nano-lattices substrate for human pluripotent stem cells culture

• Project/Area Number: 24651136
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Kyoto University
• Principal Investigator: CHEN Yong 京都大学, 物質-細胞統合システム拠点, 教授 (70512458)
• Co-Investigator(Kenkyū-buntansha): LI Liu 京都大学, 物質-細胞統合システム拠点, 助教 (50380093)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 3次元 / スカフォード / ヒト多能性幹細胞 / フィーダーフリ / ３次元

Research Abstract

Human pluripotent stem cells (hPSCs) are useful resources fortissue engineering, drug screening, and regenerative medicine. Cellular microenvironmental cues play critical roles in regulating hPSCs functions, but it is challenging to control them with synthetic components. In this project, we focus to develop a 3D nano-lattices scaffold for maintaining pluripotent stem cell culture based on micro-engineering tools and techniques. hPSCs on 3D nano-lattics scaffold showed a growth rate and they retained their pluripotency.

Research Products

• [Journal Article] Nanofibrous gelatin substrates for long-term expansion of human pluripotent stem cells (2014)
  • Author(s): Li Liu, Momoko Yoshioka, Minako Nakajima, Arata Ogasawara, Jun Liu, Kouichi Hasegawa, Sisi Li, Jianli Zou, Norio Nakatsuji, Ken-ichiro Kamei, Yong Chen
  • Journal Title: Biomaterial Volume: 35 Pages: 6259-6267
• [Journal Article] Nanofibrous gelatin substrates for long-term expansion of human pluripotent stem cells (2014)
  • Author(s): Li Liu, Momoko Yoshioka, Minako Nakajima, Arata Ogasawara, Jun Liu, Kouichi Hasegawa, Sisi Li, Jianli Zou, Norio Nakatsuji, Ken-ichiro Kamei*, Yong Chen*
  • Journal Title: Biomaterial Volume: in press Issue: 24 Pages: 6259-6267
  • DOI: 10.1016/j.biomaterials.2014.04.024
  • Peer Reviewed
• [Journal Article] Fabrication of gelatin nanopatterns for cell culture studies (2013)
  • Author(s): S. S. Li, J. Shi, L. Liu, J.J. Li, L. M. Jiang, C. X. Luo, K. Kamei, Y. Chen
  • Journal Title: Microelectron. Eng Volume: 110 Pages: 70-74
• [Journal Article] Influence of patterned topographic features on the formation of cardiac cell clusters and their rhythmic activities (2013)
  • Author(s): L. Wang, L. Liu, N. Magome, K. Agladzea, Y. Chen
  • Journal Title: Biofabrication Volume: 5(3)
• [Journal Article] Fabrication of gelatin nanopatterns for cell culture studies (2013)
  • Author(s): Sisi Li
  • Journal Title: Microelectron. Eng. Volume: in press Pages: 70-74
  • DOI: 10.1016/j.mee.2013.01.053
  • Peer Reviewed
• [Presentation] Combinatorial screening of gelatin nanofibers as cellular scaffolds to expand hPSCs for long-term period (2013)
  • Author(s): Li Liu, Ken-ichiro Kamei, Yong Chen
  • Organizer: 11th International Society for Stem Cell Research (ISSCR) Annual Meeting
  • Place of Presentation: Boston (USA)
• [Remarks]
  • URL: http://www.chen.icems.kyoto-u.ac.jp
• [Remarks] Chen Group, iCeMS, Kyoto University
  • URL: http://www.chen.icems.kyoto-u.ac.jp/
• [Patent(Industrial Property Rights)] ファイバー・オン・ファイバーを用いた多能性幹細胞の3次元培養方法及びそのための基材 (2013)
  • Inventor(s): Ken-ichiro Kamei, Norio Nakatsuji, Li Liu, Yong Chen
  • Industrial Property Rights Holder: 京都大学
  • Industrial Property Number: 2013-117242
  • Filing Date: 2013-06-03
  • Overseas
• [Patent(Industrial Property Rights)] 多能性幹細胞の培養方法及びそのための基材 (2012)
  • Inventor(s): Li Liu, Ken-ichiro Kamei, Yong Chen
  • Industrial Property Rights Holder: 京都大学
  • Industrial Property Number: 2012-127580
  • Filing Date: 2012-06-04