Development of novel labeling technique for macro molecule structure analysis
| Project/Area Number |
24651151
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Kyushu University |
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Principal Investigator |
MAYANAGI Kouta 九州大学, 生体防御医学研究所, 助教 (50418571)
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| Co-Investigator(Renkei-kenkyūsha) |
ISHINO Yoshizumi 九州大学, （連合）農学研究科（研究院）, 教授 (30346837)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 電子顕微鏡 / ナノバイオ / 可視化 / 生物物理 / 蛋白質 / DNA / 分子標識 / 画像解析 |
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| Outline of Final Research Achievements |
Molecular labeling is a key technique for mapping individual components in the 3D map of supermolecules, obtained by single particle analysis. We developed novel methods of labeling DNA in the DNA-protein complexes, which are biologically very important. We used streptavidin in our previous work, which was too large (about 60k for tetramer) for more detailed analysis.
In this project we tried mainly two methods. The first uses the nano structures of DNA for labeling. Using the pinpoint shape of the nano-structure, we could develop a label, which could point the DNA terminal more precisely.
Another label is a small bulky structure, the size of which is comparable to the resolution of the single particle analysis. As the second method seemed to be more promising, we applied the later method to the protein-DNA complexes labeling experiment, and could successfully visualize the label in the total map of the complex.
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Report
(3 results)
Research Products
(12 results)
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[Presentation] Divergently evolved activation mechanism of the replicative helicase in the thermophilic archaeon, Thermoplasma acidophilum2013
Author(s)
Hiromi Ogino, Sonoko Ishino, Kouta Mayanagi, Takuji Oyama, Tsuyoshi Shirai, Kosuke Morikawa, Gyri Teien Haugland, Nils-Kare Birkeland, Daisuke Kohda, Yoshizumi Ishino
Organizer
第36回日本分子生物学会
Place of Presentation
神戸
Year and Date
2013-12-03 – 2013-12-06
Related Report
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[Presentation] Activation mechanism of the replicative helicase in the thermophilic archaeon, Thermoplasma acidophilum2013
Author(s)
Hiromi Ogino, Sonoko Ishino, Kouta Mayanagi, Takuji Oyama, Tsuyoshi Shirai, Kosuke Morikawa, Gyri Teien Haugland, Nils-Kare Birkeland, Daisuke Kohda, Yoshizumi Ishino
Organizer
Thermophiles 2013
Place of Presentation
Regensburg, Germany
Year and Date
2013-09-08 – 2013-09-13
Related Report
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