• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of an organ on a chip that mimics the ocular fundus

Research Project

Project/Area Number 24651156
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Microdevices/Nanodevices
Research InstitutionTohoku University

Principal Investigator

KAJI Hirokazu  東北大学, 工学(系)研究科(研究院), 准教授 (70431525)

Co-Investigator(Kenkyū-buntansha) ABE Toshiaki  東北大学, 大学院医学系研究科, 教授 (90191858)
NAGAI Nobuhiro  東北大学, 大学院医学系研究科, 教授 (30400039)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsシステムオンチップ / 生体材料 / マイクロ・ナノデバイス / 細胞・組織
Research Abstract

Developing new three-dimensional (3D) in vitro cell culture models closely mimicking human tissues and organs (referred to as "organ on a chip") has recently attracted great attention for disease modeling and drug discovery. In particular, there have been many attempts to mimic the digestive and respiratory systems such as the lung, liver and intestine. To date, however, there are few reported on on-chip culture models of sensory organs such as the eye. Thus, this research aims to develop a cell-based retinal model within a microfluidic device and to examine cellular responses to controlled environments. The characterization of neural supporting cells including retinal pigment epithelial cells cultured in the device was performed and found to exhibit properties found in native tissues. Application of stimuli that induce pathological changes in cultured cells would extend the system to a disease model for new therapy and drug discovery.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (42 results)

All 2014 2013 2012 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (30 results) (of which Invited: 4 results) Book (6 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] A Platform for Controlled Dual-Drug Delivery to the Retina : Protective Effects against Light-Induced Retinal Damage in Rats2014

    • Author(s)
      N Nagai, H Kaji, H Onami, Y Katsukura, Y Ishikawa, ZK Nezhad, K Sampei, S Iwata, S Ito, M Nishizawa, T Nakazawa, N Osumi, Y Mashima, T Abe
    • Journal Title

      Advanced Healthcare Materials

      Volume: in press Issue: 10 Pages: 680-687

    • DOI

      10.1002/adhm.201400114

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Micropatterned polymeric nanosheets for local delivery of an engineered epithelial monolayer2014

    • Author(s)
      Toshinori Fujie, Yoshihiro Mori, Shuntaro Ito, Matsuhiko Nishizawa, Hojae Bae, Nobuhiro Nagai, Hideyuki Onami, Toshiaki Abe, Ali Khademhosseini, Hirokazu Kaji
    • Journal Title

      Adv. Mater

      Volume: 26 Issue: 29 Pages: 1699-1705

    • DOI

      10.1002/adma.201301300

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A polymeric device for controlled transscleral multi-drug delivery to the posterior segment of the eye2014

    • Author(s)
      Nobuhiro Nagai, Hirokazu Kaji, Hideyuki Onami, Yumi Ishikawa, Matsuhiko Nishizawa, Noriko Osumi, Toru Nakazawa, Toshiaki Abe
    • Journal Title

      Acta Biomaterialia

      Volume: 10(2) Issue: 2 Pages: 680-6877

    • DOI

      10.1016/j.actbio.2013.11.004

    • NAID

      40020425298

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transscleral sustained vasohibin-1 delivery by a novel device suppressed experimentally-induced choroidal neovascularization2013

    • Author(s)
      Hideyuki Onami, Nobuhiro Nagai, Hirokazu Kaji, Matsuhiko Nishizawa, Yasufumi Sato, Noriko Osumi, Toru Nakazawa, Toshiaki Abe
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 3 Pages: e58580-e58580

    • DOI

      10.1371/journal.pone.0058580

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biomimetic tissues on a chip for drug discovery2012

    • Author(s)
      Amir M. Ghaemmaghami, Matthew J. Hancock, Helen Harrington, Hirokazu Kaji, Ali Khademhosseini
    • Journal Title

      Drug Discov. Today

      Volume: 17 Issue: 3-4 Pages: 173-181

    • DOI

      10.1016/j.drudis.2011.10.029

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 眼科領域におけるマイクロ・ナノ技術応用2014

    • Author(s)
      梶弘和
    • Organizer
      第26回バイオエンジニアリング講演会
    • Place of Presentation
      仙台(基調講演)
    • Related Report
      2013 Final Research Report
  • [Presentation] 眼科領域におけるマイクロ・ナノ技術応用2014

    • Author(s)
      梶 弘和
    • Organizer
      第26回バイオエンジニアリング講演会
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] バルジ試験機構を用いた網膜色素上皮細胞評価システムの構築2013

    • Author(s)
      伊藤俊太郎,綱嶋俊一,藤枝俊宣,永井展裕,長峯邦明,西澤松彦,阿部俊明,梶弘和
    • Organizer
      バイオ・マイクロシステム研究会
    • Place of Presentation
      東京大学
    • Year and Date
      2013-10-08
    • Related Report
      2013 Final Research Report
  • [Presentation] 自己支持性ナノシートによる細胞輸送システムの開発2013

    • Author(s)
      森好弘,藤枝俊宣,永井展裕,西澤松彦,阿部俊明,梶弘和
    • Organizer
      バイオ・マイクロシステム研究会
    • Place of Presentation
      東京大学
    • Year and Date
      2013-10-08
    • Related Report
      2013 Final Research Report
  • [Presentation] マイクロ流路デバイスを用いた眼底組織模倣チップの開発2013

    • Author(s)
      伊藤俊太郎,永井典裕,長峯邦明,西澤松彦,阿部俊明,梶弘和
    • Organizer
      日本機械学会東北支部第48期総会・講演会
    • Place of Presentation
      仙台(独創研究学生賞受賞)
    • Year and Date
      2013-03-15
    • Related Report
      2013 Final Research Report
  • [Presentation] マイクロ流体デバイスを用いる眼底組織培養モデルの開発2013

    • Author(s)
      梶弘和
    • Organizer
      日本動物実験代替法学会第26回大会
    • Place of Presentation
      京都テルサ
    • Related Report
      2013 Final Research Report
  • [Presentation] Polymeric ultra-thin films for local delivery of cell monolayer2013

    • Author(s)
      Yoshinori Mori, Toshinori Fujie, Nobuhiro Nagai, Matsuhiko Nishizawa, Toshiaki Abe, Ali Khademhosseini, Hirokazu Kaji
    • Organizer
      12th US-Japan Symposium on Drug Delivery Systems
    • Place of Presentation
      Maui, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Development of a cell-based model of the ocular fundus within a microfluidic device2013

    • Author(s)
      Syuntaro Ito, Shunichi Tsunajima, Toshinori Fujie, Nobuhiro Nagai, Kuniaki Nagamine, Matsuhiko Nishizawa, Toshiaki Abe, Hirokazu Kaji
    • Organizer
      12th US-Japan Symposium on Drug Delivery Systems
    • Place of Presentation
      Maui, USA
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] バルジ試験器機構を利用した上皮細胞への力学的負荷システムの開発2013

    • Author(s)
      綱嶋俊一,伊藤俊太郎,藤枝俊宣,永井展裕,西澤松彦,阿部俊明,梶弘和
    • Organizer
      化学とマイクロ・ナノシステム学会第28回研究会
    • Place of Presentation
      姫路
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] ナノシートによる細胞輸送システムの開発2013

    • Author(s)
      森好弘,藤枝俊宣,永井展裕,西澤松彦,阿部俊明,梶弘和
    • Organizer
      化学とマイクロ・ナノシステム学会第28回研究会
    • Place of Presentation
      姫路
    • Related Report
      2013 Final Research Report
  • [Presentation] 眼底組織モデル構築に向けたマイクロ流路デバイスの開発2013

    • Author(s)
      伊藤俊太郎,綱島俊一,藤枝俊宣,永井展裕,長峯邦明,西澤松彦,阿部俊明,梶弘和
    • Organizer
      第35回日本バイオマテリアル学会大会
    • Place of Presentation
      船堀
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Local delivery of engineered cell monolayer directed by micropatterned polymeric nanosheets2013

    • Author(s)
      Toshinori Fujie, Yoshihiro Mori, Toshiaki Abe, Hirokazu Kaji, Ali Khademhosseini
    • Organizer
      第35回日本バイオマテリアル学会大会
    • Place of Presentation
      船堀
    • Related Report
      2013 Final Research Report
  • [Presentation] ナノシートを用いる細胞送達システムの開発2013

    • Author(s)
      森好弘,藤枝俊宣,永井展裕,西澤松彦,阿部俊明,梶弘和
    • Organizer
      第35回日本バイオマテリアル学会大会
    • Place of Presentation
      船堀(ハイライト講演に選定)
    • Related Report
      2013 Final Research Report
  • [Presentation] Retinal pigment epithelial cells supported by polymeric ultra-thinfilms for the subretinal delivery2013

    • Author(s)
      Hirokazu Kaji, Toshinori Fujie, Yoshihiro Mori, Nobuhiro Nagai, Matsuhiko Nishizawa, Ali Khademhosseini, Toshiaki Abe
    • Organizer
      International Soft Matter Conference 2013
    • Place of Presentation
      Rome, Italy
    • Related Report
      2013 Final Research Report
  • [Presentation] Flexible nanomembranes for directing cellular organization2013

    • Author(s)
      Toshinori Fujie, Hao Liu, Ken Nakajima, Hirokazu Kaji, Ali Khademhosseini
    • Organizer
      International Soft Matter Conference 2013
    • Place of Presentation
      Rome, Italy
    • Related Report
      2013 Final Research Report
  • [Presentation] Protective effects of Clotrimazole against oxidative stress-induced cell death in RGC-5 cells and preparation of controlled release device2013

    • Author(s)
      Zhaleh Kashkouli Nezhad, Nagai Nobuhiro, Yamamoto Kotaro, Saya Hideyuki, Kaji Hirokazu, Nishizawa Matsuhiko, Nakazawa Toru, Abe Toshiaki
    • Organizer
      第29回DDS学会学術集会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] マイクロ流路デバイスを用いる眼底組織モデルの開発2013

    • Author(s)
      伊藤俊太郎,永井典裕,長峯邦明,西澤松彦,阿部俊明,梶弘和
    • Organizer
      化学とマイクロ・ナノシステム学会第27回研究会
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] マイクロ流路デバイスを用いる眼底組織モデルの開発2013

    • Author(s)
      伊藤俊太郎,永井典裕,長峯邦明,西澤松彦,阿部俊明,梶弘和
    • Organizer
      電気化学会第80回大会
    • Place of Presentation
      仙台
    • Related Report
      2013 Final Research Report
  • [Presentation] マイクロ流体デバイスを用いる眼底組織培養モデルの開発2013

    • Author(s)
      梶 弘和
    • Organizer
      日本動物実験代替法学会第26回大会
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] マイクロ流路デバイスを用いた眼底組織模倣チップの開発2013

    • Author(s)
      伊藤俊太郎
    • Organizer
      日本機械学会東北支部第48期総会・講演会
    • Place of Presentation
      仙台
    • Related Report
      2012 Research-status Report
  • [Presentation] Development of a cell-based model of the retina within a microfluidic device2012

    • Author(s)
      Hirokazu Kaji, Shuntaro Ito, Nobuhiro Nagai, Kuniaki Nagamine, Matsuhiko Nishizawa, Toshiaki Abe
    • Organizer
      Symposium on New Technology for Cell-based Drug Assay
    • Place of Presentation
      Tokyo
    • Year and Date
      2012-12-10
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] マイクロ流体デバイスを用いる眼底組織培養モデルの開発2012

    • Author(s)
      梶弘和
    • Organizer
      日本動物実験代替法学会第25回大会
    • Place of Presentation
      慶応義塾大学薬学部芝共立キャンパス
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] An implantable drug delivery device for treating retinal disorders2012

    • Author(s)
      Hirokazu Kaji, Nobuhiro Nagai, Takuya Yamada, Matsuhiko Nishizawa, Toshiaki Abe
    • Organizer
      IEEE-EMBS Micro- and Nanoengineering in Medicine Conference
    • Place of Presentation
      Maui, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Ultra-thin polymeric interface to engineer muscle tissues2012

    • Author(s)
      Toshinori Fujie, Hirokazu Kaji, Ali Khademhosseini
    • Organizer
      IEEE-EMBS Micro- and Nanoengineering in Medicine Conference
    • Place of Presentation
      Maui, USA(Poster award)
    • Related Report
      2013 Final Research Report
  • [Presentation] バイオファブリケーション技術の開発とオーガンチップデバイスへの展開2012

    • Author(s)
      梶弘和
    • Organizer
      日本バイオマテリアル学会シンポジウム2012
    • Place of Presentation
      仙台
    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Invited
  • [Presentation] マイクロ流路デバイスを用いる眼底組織培養モデルの開発2012

    • Author(s)
      伊藤俊太郎,永井典裕,長峯邦明,西澤松彦,阿部俊明,梶弘和
    • Organizer
      日本バイオマテリアル学会シンポジウム2012
    • Place of Presentation
      仙台
    • Related Report
      2013 Final Research Report
  • [Presentation] Biofabrication techniques for biologically relevant tissue models and drug delivery devices2012

    • Author(s)
      Hirokazu Kaj
    • Organizer
      23rd 2012 International Symposium on Micro-NanoMechatronics and Human Science
    • Place of Presentation
      Nagoya, Japan
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] A controlled-release capsule device for transscleral drug delivery to the retina2012

    • Author(s)
      Hirokazu Kaji, Nobuhiro Nagai, Takuya Yamada, Matsuhiko Nishizawa, Toshiaki Abe
    • Organizer
      μTAS 2012
    • Place of Presentation
      Okinawa, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Biofabrication techniques for biologically relevant tissue models and drug delivery devices2012

    • Author(s)
      Hirokazu Kaji
    • Organizer
      23rd 2012 International Symposium on Micro-NanoMechatronics and Human Science
    • Place of Presentation
      Nagoya
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] バルジ試験機構を用いた網膜色素上皮細胞評価システムの構築

    • Author(s)
      伊藤俊太郎, 綱嶋俊一, 藤枝俊宣, 永井展裕, 長峯邦明, 西澤松彦, 阿部俊明, 梶 弘和
    • Organizer
      バイオ・マイクロシステム研究会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Book] "Bioinspired Muscle Tissue Devices" In Handbook of Biomimetics and Bioinspiration2014

    • Author(s)
      Toshinori Fujie, Serge Ostrovidov, Samad Ahadian, S. Prakash Parthiban, Ali Khademhosseini, Hirokazu Kaji(E. Jabbari, A. Khademhosseini, L. P. Lee, D.-H. Kim, A. Ghaemmaghami Eds)
    • Publisher
      World Scientific Publishing(in press)
    • Related Report
      2013 Final Research Report
  • [Book] "Microfluidic systems for controlling stem cell microenvironments" In Microfluidic Cell Culture Systems, 2nd Edition2014

    • Author(s)
      Seila Selimovic, Hirokazu Kaji, Hojae Bae, Ali Khademhosseini(J. T. Borenstein, C. J. Bettinger, S. L. Tao, Eds)
    • Publisher
      Elsevier Publishing(in press)
    • Related Report
      2013 Final Research Report
  • [Book] Microfluidic systems for controlling stem cell microenvironments” In Microfluidic Cell Culture Systems, 2nd Edition2014

    • Author(s)
      Seila Selimovic, Hirokazu Kaji, Hojae Bae, Ali Khademhosseini
    • Publisher
      Elsevier Publishing
    • Related Report
      2013 Annual Research Report
  • [Book] MRS Symposium Proceesings Volume 1415, MEMS, BioMEMS andBioelectronics – Materials and Devices2012

    • Author(s)
      Tim Albrecht, Maarten P. de Boer, Frank W. DelRio, Mehmet R. Dokmeci, Cristoph Eberl, Junji Fukuda, Hirokazu Kaji, Chris Keimel, Ali Khademhosseini
    • Total Pages
      221
    • Publisher
      Cambridge University Press
    • Related Report
      2013 Final Research Report
  • [Book] "Impedance sensing of biological processes in mammalian cells" In Integrated Biomaterials for Biomedical Technology2012

    • Author(s)
      Lamya Ghenim, Hirokazu Kaji, Matsuhiko Nishizawa, Xavier Gidrol(M. Ramalingam, A. Tiwari, S. Ramakrishna, H. Kobayashi, Eds)
    • Publisher
      Scrivener Publishing
    • Related Report
      2013 Final Research Report
  • [Book] MEMS, BioMEMS and Bioelectronics – Materials and Devices2012

    • Author(s)
      Tim Albrecht
    • Publisher
      Cambridge University Press
    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 細胞担持パターン化ナノ薄膜2013

    • Inventor(s)
      梶 弘和,藤枝俊宣,森 好弘,西澤松彦,阿部俊明,永井展裕
    • Industrial Property Rights Holder
      国立大学法人東北大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-06-28
    • Related Report
      2013 Final Research Report
    • Overseas

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi