| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Three-dimensional polystyrene (PS) microfiber fabric with vacuum system was developed for capturing circulating tumor cells (CTCs) within a short time. Various microfiber fabrics with different diameters were prepared by the electrospinning method and optimized for contact frequency of cells. The vacuum filtration utilizing these microfiber fabrics could filter cells without mechanical damage. The fabric system with immobilized anti-EpCAM antibodies was able to specifically capture MCF-7 cells that express EpCAM on their cell fabric within seconds. Also, the specificity of the system was tested by monitoring the ability to isolate MCF-7 cells from a mixture with CCRF-CEM cells that do not express EpCAM. Furthermore, the capture ability of the microfiber remained intact even when tested with whole blood of pigs spiked with MCF-7 cells.
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