Development of aggregation control protein crystallization apparatus

• Project/Area Number: 24651164
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Microdevices/Nanodevices
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: MIYATAKE Hideyuki 独立行政法人理化学研究所, 連携支援ユニット, 専任研究員 (50291935)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: タンパク質結晶 / タンパク質結晶化装置 / ナノ会合制御 / タンパク質結晶化 / タンパク質分子会合制御 / X線結晶構造解析 / タンパク質分子分散制御

Research Abstract

We developed a solution circulating crystallization apparatus, which can grow protein crystals in the rational way. We can control the nano-aggregation of the protein molecules by changing the solution composition in the apparatus, which optimizes the chance of crystallization. We observed the sample protein crystallization with the apparatus.

Research Products

• [Journal Article] Real-Time Control of Nanoscale Protein Assembly for Further Crystallization Using a Solution Circulating Nanoaggregation Control Apparatus (2012)
  • Author(s): Miyatake, H,et al
  • Journal Title: Crystal Growth & Design Volume: 12 Issue: 9 Pages: 4466-4477
  • DOI: 10.1021/cg300619d
  • Peer Reviewed
• [Presentation] Protein crystallization by real-time controlling of solution composition (2013)
  • Author(s): Hideyuki Miyatake, Tomohiro Yano, Ryo Shiroma, Nobuo Fukumoto, Kazuki Matsumoto, Masahide Kobayashi, Shin Maezawa, Naohiro Kuriyama
  • Organizer: 第86回日本生化学会
  • Place of Presentation: パシフィコ横浜、日本
• [Presentation] Real-time control of protein aggregation for further rational crystallization (2013)
  • Author(s): Hideyuki Miyatake, Tomohiro Yano, Ryo Shiroma, Nobuo Fukumoto, Kazuki Matsumoto, Masahide Kobayashi, Shin Maezawa, Naohiro Kuriyama
  • Organizer: ICSG2013-SLS
  • Place of Presentation: Sapporo, Japan
• [Presentation] Development of a DLS-based protein crystallization apparatus (2013)
  • Author(s): Miyatake, H
  • Organizer: 46th Erice Course
  • Place of Presentation: Erice, Italy
• [Presentation] Development of a DLS-based protein crystallization apparatus (2013)
  • Author(s): Miyatake, H.
  • Organizer: 46th Erice coursae
  • Place of Presentation: Erice, Italy
• [Presentation] Real-time control of protein aggregation for further rational crystalization (2013)
  • Author(s): Hideyuki Miyatake*, Tomohiro Yano, Ryo Shiroma, Nobuo Fukumoto2, Kazuki Matsumoto, Masahide Kobayashi, Shin Maezawa, Naohiro Kuriyama
  • Organizer: ICSG2013-SLS
  • Place of Presentation: Sappolo, Japan
• [Presentation] Protein crystallization by real-time controlling of solution composition (2013)
  • Author(s): Hideyuki Miyatake*, Tomohiro Yano, Ryo Shiroma, Nobuo Fukumoto2, Kazuki Matsumoto, Masahide Kobayashi, Shin Maezawa, Naohiro Kuriyama
  • Organizer: 第86回 日本生化学大会
  • Place of Presentation: パシフィコ横浜、日本
• [Presentation] 新手法を用いたマイクロフロー型自動タンパク質結晶化システムの紹介 (2013)
  • Author(s): 宮武秀行
  • Organizer: 日本薬学会年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Development of a DLS-based solution circulating crystallization apparatus (2012)
  • Author(s): Miyatake, H.
  • Organizer: ICCBM14
  • Place of Presentation: USA, Huntsville AL
• [Presentation] マイクロフロー型タンパク質自動結晶化システム (2012)
  • Author(s): 宮武秀行
  • Organizer: JASIS 2012
  • Place of Presentation: 幕張メッセ
• [Presentation] マイクロリアクターを用いた、新規タンパク質結晶化装置の開発 (2012)
  • Author(s): 宮武秀行
  • Organizer: 京都大学マクロ化学生産研究コンソーシアム
  • Place of Presentation: 京大・桂キャンパス
  • Invited