Creation of biological-artificial hybrid rotary nanomotor

• Project/Area Number: 24651167
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Microdevices/Nanodevices
• Research Institution: The University of Tokyo
• Principal Investigator: IINO Ryota 東京大学, 工学(系)研究科(研究院), 准教授 (70403003)
• Co-Investigator(Kenkyū-buntansha): UCHIHASHI Takayuki 金沢大学, 数物科学系, 准教授 (30326300)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ナノマシン / 分子モーター / １分子計測 / １分子観察・操作

Research Abstract

DNA-F1, a hybrid motor which has double stranded DNA as a rotor inserted into the stator ring of F1-ATPase was generated. Dependent on the energy of ATP, DNA-F1 showed stepping rotation highly biased to the one direction. Although the efficiency of the unidirectionality and the frequency of finding rotation are still low, we have achieved the first step creating hybrid rotary nanomotor. Furthermore, as a material of hybrid motor, we have constructed the single-molecule rotation assay of V1-ATPase from Enterococcus hirae and basic properties of rotary dynamics have been revealed.

Research Products

• [Journal Article] Biased Brownian stepping rotation of FoF1-ATP synthase driven by proton motive force (2013)
  • Author(s): Watanabe R, Tabata KV, Iino R, Ueno H, Iwamoto M, Oiki S, Noji H
  • Journal Title: Nature Communications Volume: 4 Issue: 1 Pages: 1-6
  • DOI: 10.1038/ncomms2631
  • Peer Reviewed
• [Journal Article] Basic properties of rotary dynamics of the molecular motor Enterococcus hirae V1-ATPase (2013)
  • Author(s): Yoshihiro Minagawa, Hiroshi Ueno, Mayu Hara, Yoshiko Ishizuka-Katsura, Noboru Ohsawa,Takaho Terada, Mikako Shirouzu, Shigeyuki Yokoyama, Ichiro Yamato, Eiro Muneyuki, Hiroyuki Noji, Takeshi Murata, and Ryota Iino
  • Journal Title: J.Biol.Chem Volume: 288 Issue: 45 Pages: 32700-32707
  • DOI: 10.1074/jbc.m113.506329
  • Peer Reviewed
• [Journal Article] Operation mechanism of F_oF_1-adenosine triphosphate synthase revealed by its structure and dynamics (2013)
  • Author(s): Iino R, Noji H
  • Journal Title: IUBMB Life Volume: 65 Issue: 3 Pages: 238-246
  • DOI: 10.1002/iub.1120
  • Peer Reviewed
• [Journal Article] Intersubunit coordination and cooperativity in ring-shaped NTPases (2013)
  • Author(s): Iino R, Noji H
  • Journal Title: Current Opinion in Structural Biology Volume: 23 Issue: 2 Pages: 229-234
  • DOI: 10.1016/j.sbi.2013.01.004
  • Peer Reviewed
• [Journal Article] DNAを巻き取る分子リール―F1-ATPaseのおもちゃ― (2013)
  • Author(s): 游慧娟,飯野亮太
  • Journal Title: 生物物理 Volume: 53 Pages: 160-161
  • Peer Reviewed
• [Journal Article] DNA を巻き取る分子リール―F1-ATPase のおもちゃ― (2013)
  • Author(s): 游 慧娟, 飯野亮太
  • Journal Title: 生物物理 Volume: 53 Pages: 160-161
  • Peer Reviewed
• [Journal Article] Molecular Mechanism of ATP Hydrolysis in F_1-ATPase Revealed by Molecular Simulations and Single-Molecule Observations (2012)
  • Author(s): Shigehiko Hayashi, Hiroshi Ueno, Abdul Rajjak Shaikh, Myco Umemura, Motoshi Kamiya, Yuko Ito, Mitsunori Ikeguchi, Yoshihiro Momoriya, Ryota Iino, Hiroyuki Noji
  • Journal Title: Journal of American Chemical Society Volume: 134 Issue: 20 Pages: 8447-8454
  • DOI: 10.1021/ja211027m
  • Peer Reviewed
• [Journal Article] Principle role of the arginine finger in rotary catalysis of F1-ATPase (2012)
  • Author(s): Komoriya Y, Ariga T, Iino R, Imamura H, Okuno D, Noji H
  • Journal Title: Journal of Biological Chemistry Volume: (First published online) Issue: 18 Pages: 15134-15142
  • DOI: 10.1074/jbc.m111.328153
  • Peer Reviewed
• [Journal Article] Rotary catalysis of the stator ring of F(1)-ATPase (2012)
  • Author(s): Iino R, Noji H
  • Journal Title: Science Volume: (First published online) Issue: 10 Pages: 1732-1739
  • DOI: 10.1016/j.bbabio.2012.03.011
  • Peer Reviewed
• [Presentation] Watching and manipulating biomolecules one at a time (2014)
  • Author(s): Ryota Iino
  • Organizer: PITTCON 2014
  • Place of Presentation: Chicago, USA(PAI-NET Session : Ultrasensitive Analytical Technologies for Biology and Chemistry)
  • Year and Date: 2014-03-03
  • Invited
• [Presentation] 天然ナノモーターと人工ナノローターの1分子ダイナミクス (2014)
  • Author(s): 飯野亮太
  • Organizer: 第三回次世代の物質科学・ナノサイエンスを探る
  • Place of Presentation: 札幌
  • Year and Date: 2014-01-10
  • Invited
• [Presentation] Watching and manipulating biomolecules one at a time (2014)
  • Author(s): Ryota Iino
  • Organizer: PITTCON 2014
  • Place of Presentation: 米国、McCormick Place, Chicago, Illinois
  • Invited
• [Presentation] 天然ナノモーターと人工ナノローターの１分子ダイナミクス (2014)
  • Author(s): 飯野亮太
  • Organizer: 第三回 次世代の物質科学・ナノサイエンスを探る
  • Place of Presentation: 北海道、北海道大学百年記念会館
  • Invited
• [Presentation] Molecular mechanism of biological nanomachines probed by single-molecule techniques (2013)
  • Author(s): Ryota Iino
  • Organizer: 12th International Conference on Atomically Controlled Surfaces, Interfaces and Nanostructures (ACSIN-12)
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2013-11-07
  • Invited
• [Presentation] You can observe a lot just by watching motions of individual molecules (2013)
  • Author(s): Ryota Iino
  • Organizer: Japanese-German Frontiers of Science Symposium
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2013-11-01
  • Invited
• [Presentation] 型顕微鏡を用いたリアルタイム1分子イメージング : 蛍光法と暗視野法 (2013)
  • Author(s): 飯野亮太
  • Organizer: 第51回生物物理学会年会オリンパスランチョンセミナー
  • Place of Presentation: 京都
  • Year and Date: 2013-10-28
  • Invited
• [Presentation] 生体分子モーターの揺らぎと機能 (2013)
  • Author(s): 飯野亮太
  • Organizer: 高等研究所「分子基盤に基づく生体機能への揺らぎとダイナミックネットワークの解明」第2回研究会
  • Place of Presentation: 京都
  • Year and Date: 2013-08-09
  • Invited
• [Presentation] Operation mechanism of rotary molecular motor F1 probed by single-molecule techniques (2013)
  • Author(s): Ryota Iino
  • Organizer: American Physical Society March Meeting 2013
  • Place of Presentation: Baltimore, USA(Session G43: Focus Session : Motor dynamics - from Single Molecules to Cells II)
  • Year and Date: 2013-03-19
  • Invited
• [Presentation] 金プローブで観た生体分子モーターの動き (2013)
  • Author(s): 飯野亮太
  • Organizer: 公益財団法人新世代研究所. 2013年度第1回バイオ単分子研究会
  • Place of Presentation: 岩手
  • Invited
• [Presentation] Basic properties of rotary dynamics of Enterococcus hirae V1-ATPase motor protein (2013)
  • Author(s): Minagawa Y, Ueno H, Hara M, Ishizuka-Katsura Y, Ohsawa N, Terada T, Shirouzu M, Yokoyama S, Noji H, Murata T, Iino R
  • Organizer: 日本生物物理学会第51回年会
  • Place of Presentation: 京都府、国立京都国際会館
• [Presentation] Direct observation of the rotation of V1-ATPase from Enterococcus hirae and its torque (2013)
  • Author(s): Ueno H, Minagawa Y, Yamato I, Murata T, Iino R, Muneyuki E
  • Organizer: 日本生物物理学会第51回年会
  • Place of Presentation: 京都府、国立京都国際会館
• [Presentation] Creation of a hybrid F1 motor with DNA as the rotor (2013)
  • Author(s): Imwamoto K, Iino R, Yamauchi R, Uchihashi T, Noji H
  • Organizer: 日本生物物理学会第51回年会
  • Place of Presentation: 京都府、国立京都国際会館
• [Presentation] Molecular mechanism of biological nanomachines probed by single-molecule techniques (2013)
  • Author(s): Ryota Iino
  • Organizer: 12th International Conference on Atomically Controlled Surfaces, Interfaces and Nanostructures (ACSIN-12)
  • Place of Presentation: 茨城県、Tsukuba International Congress Center, Tsukuba
  • Invited
• [Presentation] You can observe a lot just by watching motions of individual molecules (2013)
  • Author(s): Ryota Iino
  • Organizer: Japanese-German Frontiers of Science Symposium
  • Place of Presentation: 京都府、Ktoyo Brighton Hotel
  • Invited
• [Presentation] 金プローブで観た生体分子モーターの動き (2013)
  • Author(s): 飯野亮太
  • Organizer: 新世代研究所 2013年度第1回バイオ単分子研究会
  • Place of Presentation: 岩手県、ホテル紫苑
• [Presentation] 倒立型顕微鏡を用いたリアルタイム１分子イメージング：蛍光法と暗視野法 (2013)
  • Author(s): 飯野亮太
  • Organizer: 第51回生物物理学会年会 オリンパスランチョンセミナー
  • Place of Presentation: 京都府、国立京都国際会館
  • Invited
• [Presentation] 生体分子モーターの揺らぎと機能 (2013)
  • Author(s): 飯野亮太
  • Organizer: 国際高等研究所 「分子基盤に基づく生体機能への揺らぎとダイナミックネットワークの解明」 第2回研究会
  • Place of Presentation: 京都府、国際高等研究所
  • Invited
• [Presentation] Operation mechanism of rotary molecular motor F1 probed by single-molecule techniques (2013)
  • Author(s): Ryota Iino
  • Organizer: American Physical Society March Meeting 2013
  • Place of Presentation: Baltimore, USA
  • Invited
• [Presentation] Rotary catalysis of the stator ring of F1-ATPase, a nanomotor made by protein (2012)
  • Author(s): Ryota Iino
  • Organizer: 1st International Conference on Emerging Advanced Nanomaterials (ICEAN)
  • Place of Presentation: Brisbane, Australia
  • Year and Date: 2012-10-23
  • Invited
• [Presentation] Rotary catalysis of the stator ring of F_1-ATPase (2012)
  • Author(s): Ryota Iino
  • Organizer: The 17th European Bioenergetics Conference (EBEC 2012)
  • Place of Presentation: Freiburg, Germany
  • Year and Date: 2012-09-16
  • Invited
• [Presentation] Rotary catalysis of the stator ring of F1-ATPase (2012)
  • Author(s): Ryota Iino
  • Organizer: The 17th European Bioenergetics Conference (EBEC 2012)
  • Place of Presentation: Freiburg, Germany
  • Invited
• [Book] 「モータータンパク質」DOJIN BIOSCIENCEシリーズ「揺らぎ・ダイナミクスと生体機能-物理化学的視点からみた生体分子-」Part III機能とダイナミクス第15章1節 (2013)
  • Author(s): 飯野亮太
  • Publisher: 化学同人
• [Book] DOJIN BIOSCIENCEシリーズ「揺らぎ・ダイナミクスと生体機能－物理化学的視点からみた生体分子－」Part III 機能とダイナミクス 第15章1節 モータータンパク質 (2013)
  • Author(s): 飯野亮太
  • Total Pages: 10
  • Publisher: 化学同人
• [Book] DOJIN BIOSCIENCEシリーズ「生体分子の動きを探る －揺らぎと生体機能－」Part IV 機能とダイナミクス 第15章1節「モータータンパク質」 (2013)
  • Author(s): 飯野亮太
  • Total Pages: 10
  • Publisher: 化学同人
• [Remarks] 1.回転分子モーターV1の動きを1分子毎に観察:構造が類似するF1とは異なるユニークな回転特性が明らかに
  • URL: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/rotary-dynamics-of-molecular-motor-v1/
• [Remarks] 回転分子モーターV1の動きを１分子毎に観察
  • URL: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/rotary-dynamics-of-molecular-motor-v1/