Establishment of a novel method to identify the hydroxymethylcytosine in single base resolution.
| Project/Area Number |
24651210
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Genome biology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
KOHDA Takashi 東京医科歯科大学, 難治疾患研究所, 准教授 (60211893)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | エピゲノム / ヒドロキシメチルシトシン |
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| Research Abstract |
DNA methylation is one of the important modifications in epigenetic regulation of mammalian genome system. Recently, the oxidation reaction of methylcytosine to hydroxymethylcytosine is discovered. Thus, it is important to establish a method to identify the hydroxymethylcytosine of the genome in single base resolution. Here I provide a novel method to identify the methylcytosine and the hydroxymethylcytosine with high accuracy (more than 95%) using DNMT1 enzyme.
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Report
(3 results)
Research Products
(2 results)
