Establishment of a novel method to identify the hydroxymethylcytosine in single base resolution.

Research Project

Project/Area Number	24651210
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Genome biology
Research Institution	Tokyo Medical and Dental University
Principal Investigator	KOHDA Takashi 東京医科歯科大学, 難治疾患研究所, 准教授 (60211893)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	エピゲノム / ヒドロキシメチルシトシン
Research Abstract	DNA methylation is one of the important modifications in epigenetic regulation of mammalian genome system. Recently, the oxidation reaction of methylcytosine to hydroxymethylcytosine is discovered. Thus, it is important to establish a method to identify the hydroxymethylcytosine of the genome in single base resolution. Here I provide a novel method to identify the methylcytosine and the hydroxymethylcytosine with high accuracy (more than 95%) using DNMT1 enzyme.