DNA amplification conserving DNA methylation patterns
| Project/Area Number |
24651214
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Genome biology
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| Research Institution | Osaka University |
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Principal Investigator |
TAJIMA Shoji 大阪大学, たんぱく質研究所, 教授 (50132931)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Hironobu 大阪大学, 蛋白質研究所, 助教 (60378891)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 生物工学 / DNAメチル化 / DNAメチル化酵素 |
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| Research Abstract |
We have succeeded in establishing the method to distinguish methylated and hydroxymethylated cytosine in a single nucleotide resolution using recombinant maintenance DNA methyltansferase Dnmt1, which favors to methylate hemi-methylated DNA.
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Report
(3 results)
Research Products
(28 results)
