| Project/Area Number |
24651224
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical genome science
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
TARUI Hiroshi 独立行政法人理化学研究所, LSAシステム構築ユニット, 上級研究員 (90342815)
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| Project Period (FY) |
2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | ゲノム / クロモソーム / 選別 / 染色体 / シーケンス / 遺伝子変異 / 染色体選別 |
|---|
| Research Abstract |
This project is aimed to develop a method for collecting identical chromosome with high purity. By weak sonication, intact and loosed chromosome could be obtained from cells arrested on metaphase. Next, chromosome isolation strategy was designed and constructed for targeted 9^<th> chromosome. At the same time, quality control strategy based on qPCR was also prepared. The term planned has been passed, however the experiment is continuously performed.
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