| Project/Area Number |
24651226
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
System genome science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ITO Takashi 東京大学, 理学(系)研究科(研究院), 教授 (90201326)
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| Project Period (FY) |
2012-04-01 – 2013-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | ゲノム解析技術 / クロマチン構造 / in vivoフットプリンティング / ジメチル硫酸 / 高感受性部位 |
|---|
| Research Abstract |
Proteins that act on genomic DNA functionally regulate the genome. Accordingly, if we can comprehensively reveal the interactions between genomic DNA and proteins in living cells, we will substantially deepen our understanding of how the genome works. As a novel method toward this goal, we tried to develop a method termed DMS-Seq that uses dimethylsulfate (DMS) and next-generation sequencing. Following the optimization of the method, we applied it to the baker's yeast as a model system and successfully detected signals on regions known to regulate gene expression. These results would suggest the feasibility of this approach.
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