| Project/Area Number |
24651230
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
System genome science
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
BESSHO Yasumasa 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (70261253)
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| Co-Investigator(Renkei-kenkyūsha) |
SAKUMURA Yuich 愛知県立大学, 情報科学部, 准教授 (50324968)
MATSUI Takaaki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (60403333)
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| Research Collaborator |
NAKAHATA Yasukazu 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (50390810)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | Notchシグナリング / ノックインマウス / 体節形成 / Notchシグナル / 遺伝子発現 / Notch / 遺伝子 / ジーンドセージ / マウス / 体節 |
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| Research Abstract |
Manipulation of intracellular signaling activity in embryos is an effective tool for analysis of dynamic phenomena in developing embryo. However, it is very difficult to alter the intracellular signaling activity stably and quantitatively. We tried to generate knockin mice in which Notch1 gene is duplicated, thereby increasing the number of Notch1 allele. By using this knockin mice, we can generate mouse embryos with various number of Notch1 allele from 0 to 4, thus we can stably and quantitatively alter the Notch signaling activity in this knockin mouse line. With this Knockin mouse the analyses of Notch signal activity in dynamic phenomena in embryo such as somitogenesis are available.
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