Study on the mutational robustness of the ribosome through interspecies exchange of rRNA genes
| Project/Area Number |
24651231
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
System genome science
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
MIYAZAKI Kentaro 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 研究グループ長 (60344123)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | リボソーム / rRNA / 16S rRNA / 23S rRNA / 変異耐性 / メタゲノム / 環境ゲノム / 機能相補 / DNAシャッフリング |
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| Research Abstract |
Because of structural and mechanistic complexity of the ribosome, it is generally believed that rRNAs coevolve with their partner ribosomal proteins. Thus, horizontal transfer should seldom occur for 16S (or 23S) rRNA genes to maintain the structural integrity of the ribosome. Despite this general thought, however, in this study, we have shown that growth of Escherichia coli prrn-Delta7, a null mutant of rrn operons, was successfully complemented with exogenous 16S (or 23S) rRNA genes from diverse lineages of bacteria that showed only ~80% identities with those of E. coli. Thus ribosome is unexpectedly robust enough to accommodate large mutations or horizontal gene transfer.
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Report
(3 results)
Research Products
(84 results)
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[Journal Article] Meeting Report : 1st International Functional Metagenomics Workshop May 7-8, 2012, St. Jacobs, Ontario, Canada2013
Author(s)
Engel K, Ashby D, Brady SF, Cowan DA, Doemer J, Edwards EA, Fiebig K, Martens EC, McCormac D, Mead DA, Miyazaki K, Moreno-Hagelsieb G, O'Gara F, Reid A, Rose DR, Simonet P, Sjöling S, Smalla K, Streit WR, Tedman-Jones J, Valla S, Wellington EMH, Wu C-C, Liles MR, Neufeld JD, Sessitsch A, Charles TC
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Journal Title
Stand Genomic Sci
Volume: 8
Pages: 106-111
Related Report
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[Journal Article] Meeting Report: 1st International Functional Metagenomics Workshop May 7–8, 2012, St. Jacobs, Ontario, Canada2013
Author(s)
Engel K, Ashby D, Brady SF, Cowan DA, Doemer J, Edwards EA, Fiebig K, Martens EC, McCormac D, Mead DA, Miyazaki K, Moreno-Hagelsieb G, O'Gara F, Reid A, Rose DR, Simonet P, Sjöling S, Smalla K, Streit WR, Tedman-Jones J, Valla S, Wellington EMH, Wu C-C, Liles MR, Neufeld JD, Sessitsch A, Charles TC
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Journal Title
Standards in Genomic Sciences
Volume: 8
Issue: 1
Pages: 106-111
DOI
Related Report
Peer Reviewed
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