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Study on the mutational robustness of the ribosome through interspecies exchange of rRNA genes

Research Project

Project/Area Number 24651231
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field System genome science
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

MIYAZAKI Kentaro  独立行政法人産業技術総合研究所, 生物プロセス研究部門, 研究グループ長 (60344123)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsリボソーム / rRNA / 16S rRNA / 23S rRNA / 変異耐性 / メタゲノム / 環境ゲノム / 機能相補 / DNAシャッフリング
Research Abstract

Because of structural and mechanistic complexity of the ribosome, it is generally believed that rRNAs coevolve with their partner ribosomal proteins. Thus, horizontal transfer should seldom occur for 16S (or 23S) rRNA genes to maintain the structural integrity of the ribosome. Despite this general thought, however, in this study, we have shown that growth of Escherichia coli prrn-Delta7, a null mutant of rrn operons, was successfully complemented with exogenous 16S (or 23S) rRNA genes from diverse lineages of bacteria that showed only ~80% identities with those of E. coli. Thus ribosome is unexpectedly robust enough to accommodate large mutations or horizontal gene transfer.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (84 results)

All 2014 2013 2012 Other

All Journal Article (23 results) (of which Peer Reviewed: 17 results) Presentation (43 results) (of which Invited: 7 results) Book (7 results) Remarks (5 results) Patent(Industrial Property Rights) (6 results) (of which Overseas: 4 results)

  • [Journal Article] Bacterial cellular engineering by genome editing and gene silencing2014

    • Author(s)
      Nakashima N, Miyazaki K
    • Journal Title

      Int J Mol Sci

      Volume: 15 Pages: 2773-2793

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 16S rRNAの「水平伝播」―異種16S rRNAによる遺伝的相補―2014

    • Author(s)
      佃美雪,佐藤允治,宮崎健太郎
    • Journal Title

      化学と生物

      Volume: 52-2 Pages: 70-72

    • Related Report
      2013 Final Research Report
  • [Journal Article] New insights into the catalytic active-site structure of multicopper oxidases.2014

    • Author(s)
      Komori H, Sugiyama R, Kataoka K, Miyazaki K, Higuchi Y, Sakurai T
    • Journal Title

      Acta Crystallogr D Biol Crystallogr

      Volume: 70 Issue: 3 Pages: 772-779

    • DOI

      10.1107/s1399004713033051

    • NAID

      120005451980

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 16SrRNAの「水平伝播」―異種16SrRNAによる遺伝的相補―2014

    • Author(s)
      宮崎 健太郎、佃 美雪、佐藤 允治
    • Journal Title

      化学と生物

      Volume: 52 Pages: 70-72

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Revisiting bacterial phylogeny : Natural and experimental evidence for horizontal gene transfer of 16S rRNA2013

    • Author(s)
      Kitahara K, Miyazaki K
    • Journal Title

      Mob Genet Elements

      Volume: 3

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Metagenomic screening for aromatic compound-responsive transcriptional regulators2013

    • Author(s)
      Uchiyama T, Miyazaki K
    • Journal Title

      PLOS ONE

      Volume: 8

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of a novelβ-glucosidase from a compost microbial metagenome with strong transglycosylation activity2013

    • Author(s)
      Uchiyama T, Miyazaki K, Yaoi K
    • Journal Title

      J Biol Chem

      Volume: 288 Pages: 18325-18334

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cloning, expression and characteristics of a novel alkalistable and thermostable xylanase encoding gene (mxyl) retrieved from compost-soil metagenome2013

    • Author(s)
      Verma D, Kawarabayasi Y, Miyazaki K, Satyanarayana T
    • Journal Title

      PLOS ONE

      Volume: 8

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Directed evolution study unveiling key sequence factors that affect translation efficiency in Escherichia coli2013

    • Author(s)
      Tsukuda M, Miyazaki K
    • Journal Title

      J Biosci Bioeng

      Volume: 116 Pages: 540-545

    • NAID

      110009685200

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] DNA fragmentation caused by an overdose of Zeocin2013

    • Author(s)
      Tsukuda M, Miyazaki K
    • Journal Title

      J Biosci Bioeng

      Volume: 116 Pages: 644-646

    • NAID

      110009685220

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Meeting Report : 1st International Functional Metagenomics Workshop May 7-8, 2012, St. Jacobs, Ontario, Canada2013

    • Author(s)
      Engel K, Ashby D, Brady SF, Cowan DA, Doemer J, Edwards EA, Fiebig K, Martens EC, McCormac D, Mead DA, Miyazaki K, Moreno-Hagelsieb G, O'Gara F, Reid A, Rose DR, Simonet P, Sjöling S, Smalla K, Streit WR, Tedman-Jones J, Valla S, Wellington EMH, Wu C-C, Liles MR, Neufeld JD, Sessitsch A, Charles TC
    • Journal Title

      Stand Genomic Sci

      Volume: 8 Pages: 106-111

    • Related Report
      2013 Final Research Report
  • [Journal Article] Metagenomic screening for aromatic compound-responsive transcriptional regulators.2013

    • Author(s)
      Uciyama T, Miyazaki K
    • Journal Title

      PLoS One

      Volume: 8 Issue: 9 Pages: e75795-e75795

    • DOI

      10.1371/journal.pone.0075795

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA fragmentation caused by an overdose of Zeocin.2013

    • Author(s)
      Tsukuda M, Miyazaki K
    • Journal Title

      J Biosci Bioeng

      Volume: 116 Issue: 5 Pages: 644-646

    • DOI

      10.1016/j.jbiosc.2013.05.004

    • NAID

      110009685220

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Directed evolution study unveiling key sequence factors that affect translation. efficiency in Escherichia coli.2013

    • Author(s)
      Tsukuda M, Miyazaki K
    • Journal Title

      J Biosci Bioeng

      Volume: 116 Issue: 5 Pages: 540-545

    • DOI

      10.1016/j.jbiosc.2013.05.013

    • NAID

      110009685200

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of a novel β-glucosidase from a compost microbial metagenome with strong transglycosylation activity.2013

    • Author(s)
      Uchiyama T, Miyazaki K, Yaoi K
    • Journal Title

      J Biol Chem

      Volume: 288 Issue: 25 Pages: 18325-18334

    • DOI

      10.1074/jbc.m113.471342

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Direct electron transfer to a metagenome-derived laccase fused to affinity tags near the electroactive copper site.2013

    • Author(s)
      Tsujimura S, Asahi M, Goda-Tsutsumi M, Shirai O, Kano K, Miyazaki K
    • Journal Title

      Phys Chem Chem Phys

      Volume: 15 Issue: 47 Pages: 20585-20589

    • DOI

      10.1039/c3cp53096e

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Meeting Report: 1st International Functional Metagenomics Workshop May 7–8, 2012, St. Jacobs, Ontario, Canada2013

    • Author(s)
      Engel K, Ashby D, Brady SF, Cowan DA, Doemer J, Edwards EA, Fiebig K, Martens EC, McCormac D, Mead DA, Miyazaki K, Moreno-Hagelsieb G, O'Gara F, Reid A, Rose DR, Simonet P, Sjöling S, Smalla K, Streit WR, Tedman-Jones J, Valla S, Wellington EMH, Wu C-C, Liles MR, Neufeld JD, Sessitsch A, Charles TC
    • Journal Title

      Standards in Genomic Sciences

      Volume: 8 Issue: 1 Pages: 106-111

    • DOI

      10.4056/sigs.3406845

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cloning, expression and characteristics of a novel alkalistable and thermostable xylanase encoding gene (Mxyl) retrieved from compost-soil metagenome.2013

    • Author(s)
      Verma D
    • Journal Title

      PLoS One

      Volume: 8 Issue: 1 Pages: e52459-e52459

    • DOI

      10.1371/journal.pone.0052459

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mutational robustness of 16S ribosomal RNA, shown by experimental horizontal gene transfer in Escherichia coli2012

    • Author(s)
      Kitahara K, Yasutake Y, Miyazaki K
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 109 Pages: 19220-19225

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] リボソームに翻訳以外の機能を発見2012

    • Author(s)
      宮崎健太郎
    • Journal Title

      産総研TODAY

      Volume: 12 Pages: 20-20

    • Related Report
      2013 Final Research Report
  • [Journal Article] Mutational robustness of 16S ribosomal RNA, shown by experimental horizontal gene transfer in Escherichia coli.2012

    • Author(s)
      Kitahara K
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 109 Issue: 47 Pages: 19220-19225

    • DOI

      10.1073/pnas.1213609109

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] リボソームに翻訳以外の機能がある ―リボヌクレアーゼ作用阻害2012

    • Author(s)
      宮崎健太郎
    • Journal Title

      生体の科学

      Volume: 63 Pages: 356-357

    • Related Report
      2012 Research-status Report
  • [Journal Article] リボソームに翻訳以外の機能を発見:16S rRNA ヘリックス41によるリボヌクレアーゼT2の阻害2012

    • Author(s)
      宮崎健太郎
    • Journal Title

      産総研TODAY

      Volume: 12 Pages: 20-20

    • Related Report
      2012 Research-status Report
  • [Presentation] Ribosomal RNAの人工水平伝播による大腸菌宿主デザイン2014

    • Author(s)
      宮崎健太郎
    • Organizer
      日本農芸化学会大会2014
    • Place of Presentation
      神奈川県
    • Year and Date
      2014-03-29
    • Related Report
      2013 Final Research Report
  • [Presentation] メタゲノム手法は培養法を凌駕するのか : 遺伝子資源取得に際しての手法間の比較2014

    • Author(s)
      末永光,水田志織,宮崎健太郎,矢追克郎
    • Organizer
      日本農芸化学会大会2014
    • Place of Presentation
      神奈川県
    • Year and Date
      2014-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] 腸内細菌目の16S rRNAの網状進化2014

    • Author(s)
      佐藤允治,宮崎健太郎
    • Organizer
      第8回日本ゲノム微生物学会年会
    • Place of Presentation
      東京都
    • Year and Date
      2014-03-07
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional metagenomics and Escherichia coli host engineering for efficient enzyme discovery2014

    • Author(s)
      宮崎健太郎
    • Organizer
      Kasetsart university Seminar
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2014-02-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Escherichia coli host engineering for efficient enzyme discovery2014

    • Author(s)
      宮崎健太郎
    • Organizer
      AMBC2014
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2014-02-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Escherichia coli host engineering for efficient enzyme discovery2014

    • Author(s)
      Miyazaki K
    • Organizer
      AMBC 2014
    • Place of Presentation
      Bangkok International Trade & Exhibition Centre (Bangkok, Thailand)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Escherichia coli host engineering for efficient enzyme discovery2014

    • Author(s)
      Miyazaki K
    • Organizer
      Kasetsart University Seminar
    • Place of Presentation
      Kasetsart University (Bangkok,Thailand)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] メタゲノム手法は培養法を凌駕するのか:遺伝子資源取得に際しての手法間の比較2014

    • Author(s)
      末永 光、水田 志織、宮崎 健 太郎、矢追 克郎
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学(神奈川県川崎市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] リボソーマルRNAの人工水平伝播による大腸菌宿主デザイン2014

    • Author(s)
      宮崎 健太郎
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学(神奈川県川崎市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 腸内細菌目の16S rRNAの網状進化2014

    • Author(s)
      佐藤 允治、宮崎 健太郎
    • Organizer
      第8回 日本ゲノム微生物学会年会
    • Place of Presentation
      東京農業大学(東京都世田谷区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] アンチセンスRNAを用いた新規カウンターセレクション技術の開発2013

    • Author(s)
      佃美雪,中島信孝,宮崎健太郎
    • Organizer
      細胞を創る研究会6.0
    • Place of Presentation
      山形県
    • Year and Date
      2013-11-14
    • Related Report
      2013 Final Research Report
  • [Presentation] Ribosomal RNAの人工水平伝播による大腸菌宿主デザイン2013

    • Author(s)
      佃美雪,宮崎健太郎
    • Organizer
      細胞を創る研究会6.0
    • Place of Presentation
      山形県
    • Year and Date
      2013-11-14
    • Related Report
      2013 Final Research Report
  • [Presentation] Escherichia coli host engineering for efficient enzyme discovery2013

    • Author(s)
      佃美雪,宮崎健太郎
    • Organizer
      CBI学会2013年大会
    • Place of Presentation
      東京都
    • Year and Date
      2013-10-28
    • Related Report
      2013 Final Research Report
  • [Presentation] Escherichia coli host engineering for efficient enzyme discovery2013

    • Author(s)
      宮崎健太郎
    • Organizer
      Enzyme Engineering XXII Conference
    • Place of Presentation
      富山県
    • Year and Date
      2013-09-24
    • Related Report
      2013 Final Research Report
  • [Presentation] リボソーム工学に基づく大腸菌の宿主機能改変2013

    • Author(s)
      宮崎健太郎
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島県
    • Year and Date
      2013-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] Escherichia coli host engineering for efficient enzyme discovery2013

    • Author(s)
      宮崎健太郎
    • Organizer
      2013 SIMB Annual Meeting
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2013-08-12
    • Related Report
      2013 Final Research Report
  • [Presentation] 大腸菌への遺伝子水平伝播実験により示された16S rRNAの種間和合性2013

    • Author(s)
      北原圭,宮崎健太郎
    • Organizer
      第10回21世紀大腸菌研究会
    • Place of Presentation
      静岡県
    • Year and Date
      2013-06-20
    • Related Report
      2013 Final Research Report
  • [Presentation] 系統ネットワーク法で見る腸内細菌目16S rRNAの網状進化2013

    • Author(s)
      佐藤允治,宮崎健太郎
    • Organizer
      第2回リボソームミーティング
    • Place of Presentation
      東京都
    • Year and Date
      2013-03-26
    • Related Report
      2013 Final Research Report
  • [Presentation] 16S rRNA置換変異による大腸菌宿主改良2013

    • Author(s)
      佃美雪,宮崎健太郎
    • Organizer
      第2回リボソームミーティング
    • Place of Presentation
      東京都
    • Year and Date
      2013-03-26
    • Related Report
      2013 Final Research Report
  • [Presentation] Ribosomal RNAの人工水平伝播による大腸菌宿主デザイン2013

    • Author(s)
      宮崎健太郎
    • Organizer
      産業微生物の力を深化させる日本の技~育種の最前線~我が国独自の起点・技術から
    • Place of Presentation
      東京都
    • Year and Date
      2013-02-17
    • Related Report
      2013 Final Research Report
  • [Presentation] Escherichia coli host engineering for efficient enzyme discovery2013

    • Author(s)
      Miyazaki K
    • Organizer
      2013 SIMB Annual Meeting
    • Place of Presentation
      Hotel Sheraton (San Diego, USA)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Escherichia coli host engineering for efficient enzyme discovery2013

    • Author(s)
      Miyazaki K
    • Organizer
      Enzyme Engineering XI Conference
    • Place of Presentation
      富山国際会議場(富山県富山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 大腸菌への遺伝子水平伝播実験により示された16SrRNAの種間和合性2013

    • Author(s)
      北原 圭、宮崎 健太郎
    • Organizer
      第10回 21世紀大腸菌研究会
    • Place of Presentation
      ホテル大観(静岡県伊豆市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 枯草菌グルコマンナンオペロンを利用したセルラーゼの探索方法2013

    • Author(s)
      劉 生浩, 五十嵐一暁, 尾崎克也, 矢追 克郎, 宮崎 健太郎
    • Organizer
      第27回セルラーゼ研究会
    • Place of Presentation
      花王霞ヶ浦研修所(茨城県稲敷郡)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 生成物阻害を受けないβ―グルコシダーゼの性状解析2013

    • Author(s)
      内山拓, 宮崎 健太郎, 矢追 克郎
    • Organizer
      第27回セルラーゼ研究会
    • Place of Presentation
      花王霞ヶ浦研修所(茨城県稲敷郡)
    • Related Report
      2013 Annual Research Report
  • [Presentation] メタゲノム由来新規β―グルコシダーゼの性状解析2013

    • Author(s)
      内山拓, 宮崎 健太郎, 矢追 克郎
    • Organizer
      日本応用糖質科学会平成25年度大会
    • Place of Presentation
      鹿児島大学(鹿児島県鹿児島市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Escherichia coli host engineering for efficient enzyme discovery2013

    • Author(s)
      佃 美雪、宮崎 健太郎
    • Organizer
      CBI学会2013年大会
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] アンチセンスRNAを用いた新規カウンターセレクション技術の開発2013

    • Author(s)
      佃 美雪、中島 信孝、宮崎 健太郎
    • Organizer
      細胞を創る研究会6.0
    • Place of Presentation
      慶応大学(山形県鶴岡市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] RibosomalRNAの人工水平伝播による大腸菌宿主デザイン2013

    • Author(s)
      佃 美雪、宮崎 健太郎
    • Organizer
      細胞を創る研究会6.0
    • Place of Presentation
      慶応大学(山形県鶴岡市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Ribosomal RNAの人工水平伝播による大腸菌宿主デザイン2013

    • Author(s)
      宮崎 健太郎
    • Organizer
      バイオインダストリー協会シンポジウム 産業微生物の力を深化させる日本の技~育種の最前線~ 我が国独自の起点・技術から
    • Place of Presentation
      東京大学(東京都文京区)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 16S rRNAの置換変異による大腸菌宿主デザイン2012

    • Author(s)
      佃美雪,宮崎健太郎
    • Organizer
      「細胞を創る」研究会5.0
    • Place of Presentation
      神奈川県
    • Year and Date
      2012-11-21
    • Related Report
      2013 Final Research Report
  • [Presentation] メタゲノムからの有用遺伝子探索2012

    • Author(s)
      宮崎健太郎
    • Organizer
      次世代シーケンス東京セミナー・メタゲノムセミナー
    • Place of Presentation
      東京都
    • Year and Date
      2012-10-25
    • Related Report
      2013 Final Research Report
  • [Presentation] 16SリボソームRNAのへリックス41はリボヌクレアーゼIの特異的インヒビターである2012

    • Author(s)
      北原圭,宮崎健太郎
    • Organizer
      第9回21世紀大腸菌研究会
    • Place of Presentation
      滋賀県
    • Year and Date
      2012-06-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional screening strategy2012

    • Author(s)
      宮崎健太郎
    • Organizer
      International Functional Metagenomics Workshop
    • Place of Presentation
      Waterloo, Canada
    • Year and Date
      2012-05-07
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional screening strategy

    • Author(s)
      宮崎健太郎
    • Organizer
      International Functional Metagenomics Workshop
    • Place of Presentation
      Stone Crock(カナダ)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] メタゲノムからの有用遺伝子探索

    • Author(s)
      宮崎健太郎
    • Organizer
      次世代シーケンス東京セミナー・メタゲノムセミナー
    • Place of Presentation
      秋葉原UDX(東京都)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 16SリボソームRNA のへリックス41はリボヌクレアーゼ I の特異的インヒビターである

    • Author(s)
      北原圭
    • Organizer
      第9回 21世紀大腸菌研究会
    • Place of Presentation
      長浜バイオ大学(滋賀県)
    • Related Report
      2012 Research-status Report
  • [Presentation] メタゲノム由来GH1β-グルコシダーゼTd-2F2のグルコース阻害耐性の構造基盤

    • Author(s)
      城俊徳
    • Organizer
      日本応用糖質科学会平成24年度大会
    • Place of Presentation
      東京農工大学(東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] 16S rRNAの置換変異による大腸菌宿主デザイン

    • Author(s)
      佃美雪
    • Organizer
      「細胞を創る」研究会5.0
    • Place of Presentation
      東京工業大学(神奈川県)
    • Related Report
      2012 Research-status Report
  • [Presentation] 16S rRNA置換変異による大腸菌宿主改良

    • Author(s)
      佃美雪
    • Organizer
      第2回リボソームミーティング
    • Place of Presentation
      東京農工大学(東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] 系統ネットワーク法で見る腸内細菌目16S rRNAの網状進化

    • Author(s)
      佐藤允治
    • Organizer
      第2回リボソームミーティング
    • Place of Presentation
      東京農工大学(東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] メタゲノム由来新奇βーグルコシダーゼの性状解析

    • Author(s)
      内山拓
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学(宮城県)
    • Related Report
      2012 Research-status Report
  • [Presentation] β-グルコシダーゼのグルコース阻害耐性と基質特異性における構造基盤

    • Author(s)
      城俊徳
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学(宮城県)
    • Related Report
      2012 Research-status Report
  • [Book] Encyclopedia of Metagenomics, extradiol dioxygenases retrieved from the metagenome2014

    • Author(s)
      Suenaga H, Miyazaki K
    • Publisher
      Springer
    • Related Report
      2013 Final Research Report
  • [Book] Diversity and Evolution of Aromatic Degradation Pathway Enzymes in an Activated Sludge2014

    • Author(s)
      Miyazaki K
    • Total Pages
      16
    • Publisher
      Springer Japan
    • Related Report
      2013 Annual Research Report
  • [Book] 進化分子工学の最前線,生物種を超えた16S rRNA遺伝子の機能相補性の解明2013

    • Author(s)
      宮崎健太郎
    • Publisher
      NTS
    • Related Report
      2013 Final Research Report
  • [Book] Extradiol dioxygenases retrieved from the metagenome2013

    • Author(s)
      Miyazaki K
    • Total Pages
      5
    • Publisher
      Springer- Verlag Berlin Heidelberg
    • Related Report
      2013 Annual Research Report
  • [Book] 生体の科学,リボソームに翻訳以外の機能がある―リボヌクレアーゼ作用阻害2012

    • Author(s)
      宮崎健太郎
    • Publisher
      医学書院
    • Related Report
      2013 Final Research Report
  • [Book] 生命システム工学,遺伝子資源の多様化2012

    • Author(s)
      宮崎健太郎
    • Publisher
      化学同人
    • Related Report
      2013 Final Research Report
  • [Book] 生命システム工学,進化分子工学の事始2012

    • Author(s)
      宮崎健太郎
    • Publisher
      化学同人
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      https://staff.aist.go.jp/miyazaki-kentaro/group/index.html

    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      https://unit.aist.go.jp/bpri/bpri-synthe/index.html

    • Related Report
      2013 Final Research Report
  • [Remarks] 産総研 生物プロセス研究部門 合成生物工学研究グループ

    • URL

      https://staff.aist.go.jp/miyazaki-kentaro/group/index.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 合成生物工学研究グループ【生物プロセス研究部門】

    • URL

      https://unit.aist.go.jp/bpri/bpri-synthe/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 生物種を越えた16S rRNA遺伝子の機能相補性を確認

    • URL

      http://www.aist.go.jp/aist_j/press_release/pr2012/pr20121030/pr20121030.html

    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 新規なカウンターセレクション法2013

    • Inventor(s)
      佃美雪,中島信孝,宮崎健太郎
    • Industrial Property Rights Holder
      佃美雪,中島信孝,宮崎健太郎
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-226229
    • Filing Date
      2013-10-31
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 酵素遺伝子スクリーニング法2013

    • Inventor(s)
      内山拓, 宮崎健太郎
    • Industrial Property Rights Holder
      内山拓, 宮崎健太郎
    • Industrial Property Rights Type
      特許
    • Acquisition Date
      2013-11-01
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 翻訳特性の改変された大腸菌2013

    • Inventor(s)
      宮崎健太郎、佃 美雪
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-03-13
    • Related Report
      2012 Research-status Report
    • Overseas
  • [Patent(Industrial Property Rights)] 翻訳特性の改変された大腸菌2012

    • Inventor(s)
      宮崎健太郎,佃美雪
    • Industrial Property Rights Holder
      宮崎健太郎,佃美雪
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2012-102128
    • Filing Date
      2012-04-27
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 翻訳特性の改変された大腸菌2012

    • Inventor(s)
      宮崎健太郎,佃美雪
    • Industrial Property Rights Holder
      宮崎健太郎,佃美雪
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-03-16
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 翻訳特性の改変された大腸菌2012

    • Inventor(s)
      宮崎健太郎、佃 美雪
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2012-102128
    • Filing Date
      2012-04-27
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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