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Development of natural products microarray for search of neural stem cell modulators

Research Project

Project/Area Number 24651238
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Living organism molecular science
Research InstitutionChiba University

Principal Investigator

ARAI Midori  千葉大学, 薬学研究科(研究院), 准教授 (40373261)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords天然物 / アレイ / ビタミンD3 / 神経幹細胞 / タンパク質
Research Abstract

Natural product extract microarray would be greatly helpful for search bioactive compounds by incubation with target proteins. Reported method needed the structural change in each natural products. In this study, the material for microarray, method for making array, incubation with target protein etc. were investigated. Poly-ether sulfone (PES) was found to be good membrane for thin-layer chromatography of extract. Vitamin D receptor and its positive control 1a, 25 (OH)2 VD3 were used for establishment of the method.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (34 results)

All 2014 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (24 results) (of which Invited: 11 results) Remarks (3 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 2 results)

  • [Journal Article] Physalin H from Solanum nigrum as Hh signaling inhibitor blocks GLI1-DNA complex formation2014

    • Author(s)
      Arai, M. A.; Uchida, K.; Sadhu, S. K.; Ahmed, F.; Ishibashi, M.
    • Journal Title

      Beil. J. Org. Chem

      Volume: 10 Pages: 134-140

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Physalin H from Solanum nigrum as Hh signaling inhibitor blocks GLI1-DNA complex formation2014

    • Author(s)
      Midori A. Arai
    • Journal Title

      Beilstein Journal of Organic Chemistry

      Volume: 10 Pages: 134-140

    • DOI

      10.3762/bjoc.10.10

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hh signaling inhibitors from Vitex negundo; naturally occurring inhibitors of the GLI1–DNA complex2013

    • Author(s)
      Arai, M. A.; Fujimatsu, T.; Uchida, K.; Sadhu, S. K.; Ahmed, F.; Ishibashi, M.
    • Journal Title

      Mol. BioSyst

      Volume: 9 Pages: 1012-1018

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Naturally Occurring Ngn2 Promoter Activators from Butea superba2013

    • Author(s)
      Arai, M. A.; Koryudzu, K.; Koyano, T.; Kowithayakorn, T.; Ishibashi, M.
    • Journal Title

      Mol. BioSyst

      Volume: 9 Pages: 2489-2497

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Catalytic Asymmetric Synthesis of Mixed 3,3'-Bisindoles and Their Evaluation as Wnt Signaling Inhibitors2013

    • Author(s)
      Arai, T.; Yamamoto, Y.; Awata, A.; Kamiya, K.; Ishibashi, M.; Arai, M. A.
    • Journal Title

      Angew. Chem. Int. Ed

      Volume: 52 Pages: 2486-2490

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] bHLH転写因子に作用する神経幹細胞分化活性化剤の探索と創成2014

    • Author(s)
      荒井緑
    • Organizer
      日本薬学会第134年会シンポジウム
    • Place of Presentation
      熊本
    • Year and Date
      2014-03-28
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Search for Neural Stem Cells Activators based on Natural Products2014

    • Author(s)
      M. A. Arai
    • Organizer
      Asian Chemical Biology Initiative 2014 Manila Meeting
    • Place of Presentation
      ベトナム
    • Year and Date
      2014-01-25
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Pandanus odoratissimusからのヘッジホッグシグナル阻害剤の探索2014

    • Author(s)
      荒井緑,赤嶺隆太,S. K. Sadhu,F. Ahmed,石橋正己
    • Organizer
      日本薬学会第134年会シンポジウム
    • Place of Presentation
      熊本
    • Related Report
      2013 Final Research Report
  • [Presentation] Themeda arguens等からのヘッジホッグ伝達経路阻害剤の探索2014

    • Author(s)
      米山達朗,荒井緑,小谷野喬,T. Kowithayakorn,S. K. Sadhu,F. Ahmed,石橋正己
    • Organizer
      日本薬学会第134年会シンポジウム
    • Place of Presentation
      熊本
    • Related Report
      2013 Final Research Report
  • [Presentation] Search for Naturally Occurring Neural Stem Cells Activators that modulate bHLH Transcriptional Factors2013

    • Author(s)
      M. A. Arai
    • Place of Presentation
      台湾中山大学(National Sun Yat-Sen University), 台湾
    • Year and Date
      2013-09-27
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Search for Naturally Occurring Neural Stem Cells Activators that modulate bHLH Transcriptional Factors2013

    • Author(s)
      M. A. Arai
    • Place of Presentation
      台湾清華大学(National Tsing Hua University), 台湾
    • Year and Date
      2013-09-25
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Search for Naturally Occurring Neural Stem Cells Activators that modulate bHLH Transcriptional Factors2013

    • Author(s)
      M. A. Arai
    • Place of Presentation
      台湾中央研究院(Academia Sinica), 台湾
    • Year and Date
      2013-09-23
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 神経幹細胞で働く転写因子Hes1ビーズを用いる迅速的天然物探索2013

    • Author(s)
      荒井緑,石川直樹,小谷野喬,T. Kowithayakorn,石橋正己
    • Organizer
      第55回天然有機化合物討論会
    • Place of Presentation
      京都
    • Year and Date
      2013-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] Search for Neural Stem Cells Activators based on Natural Products2013

    • Author(s)
      M. A. Arai
    • Organizer
      新学術領域研究「天然物ケミカルバイオロジー : 分子標的と活性制御」地区ミニシンポジウム
    • Place of Presentation
      慶應大学
    • Year and Date
      2013-03-21
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Search for Cancer Cells Signaling Inhibitors and Neural Stem Cells Activators based on Natural Products2013

    • Author(s)
      M. A. Arai
    • Organizer
      Asian Chemical Biology Initiative 2013 Bangkok Meeting
    • Place of Presentation
      タイ
    • Year and Date
      2013-01-27
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 天然物を基盤とした神経幹細胞活性化小分子の創製に向けて2013

    • Author(s)
      荒井緑
    • Organizer
      筑波大学学際研究センター物質科学第16回機能性分子シンポジウム
    • Place of Presentation
      筑波
    • Year and Date
      2013-01-12
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Vitex negundoから単離したヘッジホッグシグナル阻害作用をもつジテルペン2013

    • Author(s)
      荒井緑,藤松輝久,内田響子,S. K. Sadhu,F. Ahmed,石橋正己
    • Organizer
      第57回香料・テルペンおよび精油化学に関する討論会
    • Place of Presentation
      さいたま
    • Related Report
      2013 Final Research Report
  • [Presentation] 神経幹細胞で働く Hes1 タンパク質への結合能を指標とする天然物の探索2013

    • Author(s)
      荒井緑,浜比嘉望美,S. K. Sadhu,F. Ahmed,石橋正己
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] bHLH転写因子に影響する天然由来の神経幹細胞分化活性化小分子2012

    • Author(s)
      荒井緑
    • Organizer
      新学術領域研究「天然物ケミカルバイオロジー : 分子標的と活性制御」第2回若手研究者ワークショップ
    • Place of Presentation
      大阪
    • Year and Date
      2012-10-30
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 疾患および再生シグナルに作用する天然物の探索2012

    • Author(s)
      石橋正己,荒井緑,當銘一文
    • Organizer
      新学術領域研究「天然物ケミカルバイオロジー~分子標的と活性制御~」第2回公開シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2012-06-18
    • Related Report
      2013 Final Research Report
  • [Presentation] 神経幹細胞分化を活性化する天然物探索 : タンパク質担持ビーズを用いる迅速的探索法2012

    • Author(s)
      荒井緑,石川直樹,小谷野喬,T. Kowithayakorn,石橋正己
    • Organizer
      日本ケミカルバイオロジー学会第7回年会
    • Place of Presentation
      京都
    • Year and Date
      2012-06-07
    • Related Report
      2013 Final Research Report
  • [Presentation] Search for naturally occurring neural stem cells activators that modulate bHLH transcriptional factors2012

    • Author(s)
      M. A. Arai
    • Organizer
      7th International Conference on Cutting-Edge Organic Chemistry in Asia
    • Place of Presentation
      Singapore(Asian Core Program Lectureship Award受賞)
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Naturally occurring neural stem cells activators by Hes1-beads-HPLC method2012

    • Author(s)
      M. A. Arai, N. Ishikawa, T. Koyano, T. Kowithayakorn, M. Ishibashi
    • Organizer
      The 12th International Kyoto Conference on New Aspects of Organic Chemistry
    • Place of Presentation
      Kyoto
    • Related Report
      2013 Final Research Report
  • [Presentation] Search for natural products based ligands targeting signaling pathways of disease and reproduction2012

    • Author(s)
      M. Ishibashi and M. A. Arai
    • Organizer
      1^<st> International Symposium on Chemical Biology of Natural Products : Target ID and Regulation of Bioactivity
    • Place of Presentation
      Kyoto
    • Related Report
      2013 Final Research Report
  • [Presentation] 神経幹細胞で働くHes1タンパク質への結合能を指標とする天然物の探索2012

    • Author(s)
      荒井緑,浜比嘉望美,S. K. Sadhu,F. Ahmed,石橋正己
    • Organizer
      日本生薬学会第59年会
    • Place of Presentation
      千葉
    • Related Report
      2013 Final Research Report
  • [Presentation] 放線菌からのNgn2プロモーター活性化剤の探索2012

    • Author(s)
      小柳津和音,荒井緑,石橋正己
    • Organizer
      日本生薬学会第59年会
    • Place of Presentation
      千葉
    • Related Report
      2013 Final Research Report
  • [Presentation] Aquilaria agallochaからのHes1担持ビーズを用いる活性天然物の迅速的探索2012

    • Author(s)
      荒井緑,石川直樹,小谷野喬,T. Kowithayakorn,石橋正己
    • Organizer
      日本生薬学会第59年会
    • Place of Presentation
      千葉
    • Related Report
      2013 Final Research Report
  • [Presentation] 神経幹細胞で働く Hes1 タンパク質への結合能を指標とする天然物の探索2012

    • Author(s)
      荒井緑,浜比嘉望美,S. K. Sadhu,F. Ahmed,石橋正己
    • Organizer
      日本生薬学会第59年会
    • Place of Presentation
      千葉
    • Related Report
      2012 Research-status Report
  • [Presentation] bHLH転写因子に作用する神経幹細胞分化活性化剤の探索と創成

    • Author(s)
      荒井緑
    • Organizer
      日本薬学会第134年会シンポジウム
    • Place of Presentation
      熊本大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Remarks]

    • URL

      http://www.p.chiba-u.ac.jp/lab/kouzou/index/html

    • Related Report
      2013 Final Research Report
  • [Remarks] 活性構造化学研究室のホームページ

    • URL

      http://www.p.chiba-u.ac.jp/lab/kouzou/index.html

    • Related Report
      2012 Research-status Report
  • [Remarks] Midori A. Arai's HP

    • URL

      http://www7b.biglobe.ne.jp/&#771;midori_arai/

    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] Ngn2プロモーター活性化剤inuboshinB2013

    • Inventor(s)
      荒井緑,小柳津和音,石橋正己
    • Industrial Property Rights Holder
      千葉大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-040146
    • Filing Date
      2013-02-28
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] フラボノイド由来Hes1プロモーター阻害剤2011

    • Inventor(s)
      荒井緑,柳瀬なつき,小柳津和音,石橋正己
    • Industrial Property Rights Holder
      千葉大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2012-526493
    • Filing Date
      2011-07-25
    • Related Report
      2013 Final Research Report
    • Overseas

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Published: 2013-05-31   Modified: 2019-07-29  

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