Rapid conformational change results in attractive interactions between biomolecules

• Project/Area Number: 24651244
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: Osaka University
• Principal Investigator: MURATA Michio 大阪大学, 理学(系)研究科(研究院), 教授 (40183652)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 配座解析 / 分子運動 / 疎水性相互作用 / 脂質二重膜 / リン脂質 / 脂肪酸 / 脂肪酸結合タンパク質 / 膜タンパク質

Research Abstract

Hydrophobic interactions are known to be essential for biomolecular recognition. In this project, our hypothesis that concerted bimolecular movements lead to stabilization of complexation was confirmed by experimental data. Fatty acid and fatty-acid binding proteins were subjected to the structural determination by X-ray analysis. The results clearly showed that fatty acids with an alkyl chain longer than C14 have highly flexible conformation around their terminal methyl group. These movements of alkyl chains hardly prevent high affinity of fatty acids, thus supporting that a rapid movements of ligand molecules do not always decrease bimolecular affinity but stabilize the ligand binding.

Research Products

• [Journal Article] Highly Efficient Preparation of Selectively Isotope Cluster-Labeled Long Chain Fatty Acids via Two Consecutive Csp3-Csp3 Cross-Coupling Reactions (2014)
  • Author(s): Lethu, S., Matsuoka, S. and Murata, M.
  • Journal Title: Org. Lett Volume: 16巻 Issue: 3 Pages: 844-847
  • DOI: 10.1021/ol4036159
  • Peer Reviewed
• [Journal Article] Coexistence of two liquid crystalline phases in dihydrosphingomyelin and dioleoyl- phosphatidylcholine binary mixtures (2014)
  • Author(s): Kinoshita, M., Matsumori, N., Murata, M.
  • Journal Title: Biochim. Biophys. Acta Volume: 1838巻 Issue: 5 Pages: 1372-1381
  • DOI: 10.1016/j.bbamem.2014.01.017
  • Peer Reviewed
• [Journal Article] Interaction kinetics of liposome-incorporated unsaturated fatty acids with fatty acid-binding protein 3 by surface plasmon resonance (2014)
  • Author(s): Maria Carmen Tan, Shigeru Matsuoka, Hikaru Ano, Hanako Ishida, Mika Hirose, Fuminori Sato, Shigeru Sugiyama, Michio Murata
  • Journal Title: Bioorganic & Medicinal Chemistry Volume: 22 Issue: 6 Pages: 1804-8
  • DOI: 10.1016/j.bmc.2014.02.001
  • Peer Reviewed
• [Journal Article] Coexistence of two liquid crystalline phases in dihydrosphingomyelin and dioleoylphosphatidylcholine binary mixtures (2014)
  • Author(s): Masanao Kinoshita, Nobuaki Matsumori, Michio Murata
  • Journal Title: Biochim. Biophys. Acta Volume: 1838 Pages: 1372-81
  • Peer Reviewed
• [Journal Article] Structure of the human-heart fatty-acid-binding protein 3 in complex with the fluorescent probe 1-anilinonaphthalene-8-sulphonic acid (2013)
  • Author(s): Mika Hirose, Shigeru Sugiyama, Hanako Ishida, Mayumi Niiyama, Daisuke Matsuoka, Toshiaki Hara, Eiichi Mizohata, Satoshi Murakami,
  • Journal Title: J. Synchrotron Rad. Volume: 20 Issue: 6 Pages: 923-8
  • DOI: 10.1107/s0909049513021298
  • Peer Reviewed
• [Journal Article] Comprehensive Molecular Motion Capture for Sphingomyelin by Site-Specific Deuterium Labeling (2012)
  • Author(s): Nobuaki Matsumori
  • Journal Title: Biochemistry Volume: 51 Issue: 42 Pages: 8363-8370
  • DOI: 10.1021/bi3009399
  • Peer Reviewed
• [Journal Article] Possible conformation of amphotericin B dimer in membrane-bound assembly as deduced from solid-state NMR (2012)
  • Author(s): Umegawa, Y., Adachi, T., Matsumori, N., Murata, M.
  • Journal Title: Bioorg. Med. Chem Volume: 20巻 Issue: 19 Pages: 5699-5704
  • DOI: 10.1016/j.bmc.2012.08.016
  • Peer Reviewed
• [Presentation] 親水性脂肪酸類縁体と脂肪酸結合タンパク質の結合様式 (2014)
  • Author(s): 大塚康平・松岡茂・廣瀬未果・佐藤文憲・溝端栄一・杉山成・井上豪・村田道雄
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-03-29
• [Presentation] Synthesis of diyne-labeled sphingomyelin and evaluation of its raft-like domain formation (2014)
  • Author(s): Cui, J., Kinoshita, M., Matsuoka, S., Sato, F., Ando, J., Yamagoshi, H., Dodo, K., Fujita, K, Sodeoka, M., Murata, M.
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-03-29
• [Presentation] 親水性脂肪酸類縁体と脂肪酸結合タンパク質の結合様式 (2014)
  • Author(s): 大塚康平・松岡茂・廣瀬未果・佐藤文憲・溝端栄一・杉山成・井上豪・村田道雄
  • Organizer: 日本化学会第９４春季年会
  • Place of Presentation: 名古屋
• [Presentation] Synthesis of diyne-labeled sphingomyelin and evaluation of its raft-like domain formation (2014)
  • Author(s): CUI, Jin; KINOSHITA, Masanao; MATSUOKA, Shigeru; SATO, Fuminori; ANDO, Jun; YAMAGOSHI, Hiroyuki; DODO, Koyuke; FUJITA, Katsumasa;
  • Organizer: 日本化学会第９４春季年会
  • Place of Presentation: 名古屋
• [Presentation] Design and Synthesis of Labeled Compounds for Conformation Analysis of Fatty Acids Bound to Proteins (2013)
  • Author(s): Lethu, S., Matsuoka, S., Murata, M.
  • Organizer: First Osaka University - EPFL International Symposium at Osaka University
  • Place of Presentation: 吹田
  • Year and Date: 2013-12-02
• [Presentation] スピンエコーMAS法を用いた脂質二重膜の化学シフト異方性測定 (2013)
  • Author(s): 梅川雄一,松岡茂,山口敏幸,村田道雄
  • Organizer: 第52回NMR討論会
  • Place of Presentation: 金沢
  • Year and Date: 2013-11-13
• [Presentation] Structure of the human muscle fatty acid-binding protein complexed with competitive inhibitor (2013)
  • Author(s): Hirose, M., Sugiyama, S., Ishida, H., Matsuoka, D., Hara, T., Mizohata, E., Inoue, T., Matsuoka, S., Murata, M.
  • Organizer: The International Conference on Structural Genomics 2013 (ICSG2013), 第7回国際構造ゲノム会議
  • Place of Presentation: 札幌
  • Year and Date: 2013-07-30
• [Presentation] 変異導入によるFABP3-脂肪酸相互作用形態の変化 (2013)
  • Author(s): 松岳大輔、松岡茂、廣瀬未果、新山真由美、佐藤文憲、溝端栄一、杉山成、井上豪、村田道雄
  • Organizer: 第13回日本蛋白質科学会年会
  • Place of Presentation: 鳥取
  • Year and Date: 2013-06-13
• [Presentation] 重水素個体NMRによる脂質ラフトにおけるスフィンゴミエリンの運動性解析 (2013)
  • Author(s): 安田智一、土川博史、松森信明、村田道雄
  • Organizer: 日本化学第93春季年会
  • Place of Presentation: 立命館大学びわこくさつキャンパス
  • Year and Date: 2013-03-25
• [Presentation] 水溶性脂肪酸ミミックの分子設計とFABP結合活性 (2013)
  • Author(s): 大塚康平、松岡茂、廣瀬未果、佐藤文憲、溝端栄一、杉山成、井上豪、村田道雄
  • Organizer: 日本化学第93春季年会
  • Place of Presentation: 立命館大学びわこくさつキャンパス
  • Year and Date: 2013-03-25
• [Presentation] Structure of the human muscle fatty acid-binding protein complexed with competitive inhibitor (2013)
  • Author(s): Mika Hirose, Shigeru Sugiyama, Hanako Ishida, Daisuke Matsuoka, Toshiaki Hara, Eiichi Mizohata, Tsuyoshi Inoue, Shigeru Matsuoka, Michio Murata
  • Organizer: The International Conference on Structural Genomics 2013 (ICSG2013)
  • Place of Presentation: 札幌
• [Presentation] 重水素個体ＮＭＲによる脂質ラフトにおけるスフィンゴミエリンの運動性解析 (2013)
  • Author(s): 安田智一、土川博史、松森信明、村田道雄
  • Organizer: 日本化学会第９３春季年会
  • Place of Presentation: 立命館大学びわこくさつキャンパス
• [Presentation] 水溶性脂肪酸ミミックの分子設計とＦＡＢＰ結合活性 (2013)
  • Author(s): 大塚康平、松岡茂、廣瀬未果、佐藤文憲、溝端栄一、杉山成、井上豪、村田道雄
  • Organizer: 日本化学会第９３春季年会
  • Place of Presentation: 立命館大学びわこくさつキャンパス
• [Presentation] 脂質ラフト形成の分子機構 (2012)
  • Author(s): 山口敏幸、安田智一、前田佳子、鈴木孝、由良理陽、松岡茂、松森信明、村田道雄
  • Organizer: 第54回天然有機化合物討論会
  • Place of Presentation: 東京農業大学世田谷キャンパス
  • Year and Date: 2012-09-19
• [Book] Structural Studies of Small Bioactive Compounds Interacting with Membranes and Proteins , in Advances in Biological Solid-State NMR : Proteins and Membrane-Active Peptides, Chapter 8 (2014)
  • Author(s): Matsuoka, S. and Murata, M.(Frances Separovic and Akira Naito (Eds.))
  • Publisher: Royal Society of Chemistry
• [Book] Structural Studies of Small Bioactive Compounds Interacting with Membranes and Proteins (2014)
  • Author(s): Ｓhigeru Matsuoka, Michio Murata
  • Total Pages: 28
  • Publisher: Royal Society of Chemistry,
• [Remarks]
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/murata/
• [Remarks] 代表者の研究室のＨＰアドレス
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/murata/