| Project/Area Number |
24651247
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | Tokushima Bunri University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 生合成 / 有用物質生産 / 分化誘導活性 / ジテルペノイド / フシコッカン / 次世代シーケンサー / Plagiochila sciophila / ケモタイプ / fusicoplagin / fusicosciophin / 苔類 / ゼニゴケ / 14-3-3タンパク |
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| Research Abstract |
Novel fusicoplagins, isolated from the leafy liverwort Plagiochila sciophila, are diterpenoids thay are potent inducers of differentiation in human myelocytic leukemia HL-60 cells. Because the fusicoplagins are a minuscule amount in the liverwort, construction of the stable supply method is a matter of great urgency. Therefore, studies aiming at the elucidation of the entire biosynthetic genes was carried out using a comprehensive transcriptome method. The predicted biosynthetic genes were discovered and the function of the diterpene cyclases was analyzed using heterologous gene expression system.
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