Comprehensive studies of genes responsible for biosynthesis of fusicoplagin diterpenoids in liverworts, and their use to development of reconstituted biosynthesis system
Project/Area Number |
24651247
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Living organism molecular science
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Research Institution | Tokushima Bunri University |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
Keywords | 生合成 / 有用物質生産 / 分化誘導活性 / ジテルペノイド / フシコッカン / 次世代シーケンサー / Plagiochila sciophila / ケモタイプ / fusicoplagin / fusicosciophin / 苔類 / ゼニゴケ / 14-3-3タンパク |
Research Abstract |
Novel fusicoplagins, isolated from the leafy liverwort Plagiochila sciophila, are diterpenoids thay are potent inducers of differentiation in human myelocytic leukemia HL-60 cells. Because the fusicoplagins are a minuscule amount in the liverwort, construction of the stable supply method is a matter of great urgency. Therefore, studies aiming at the elucidation of the entire biosynthetic genes was carried out using a comprehensive transcriptome method. The predicted biosynthetic genes were discovered and the function of the diterpene cyclases was analyzed using heterologous gene expression system.
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Report
(3 results)
Research Products
(26 results)
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[Journal Article] Seed Dormancy Breaking Diterpenoids from the Liverwort Plagiochila sciophila and their Differentiation Inducing Activity in Human Acute Promyelocytic Leukemia HL-60 Cells2014
Author(s)
Kenmoku, H., Tada, H., Oogushi, M., Esumi, T., Takahashi, T., Noji, M., Sassa, T., Toyota, M., Asakawa, Y.
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Journal Title
Natural Product Communications
Volume: 9(in printing)
Related Report
Peer Reviewed
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[Journal Article] Seed Dormancy Breaking Diterpenoids, Including Novel Brassicicenes J and K, from Fungus Alternaria brassicicola, and their Necrotic/Apoptotic Activities in HL-60 Cells2014
Author(s)
Kenmoku, H., Takeue, S., Oogushi, M., Yagi, Y., Sassa, T., Toyota, M., Asakawa, Y.
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Journal Title
Natural Product Communications
Volume: 9
Pages: 351-354
Related Report
Peer Reviewed
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[Journal Article] Seed Dormancy Breaking Diterpenoids, Including Novel Brassicicenes J and K, from Fungus Alternaria brassicicola, and their Necrotic/Apoptotic Activities in HL-60 Cells.2014
Author(s)
Kenmoku, H., Takeuea, S., Oogushi, M., Yagi, Y., Takeshi Sassa, T., Toyota, M., Asakawa, Y
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Journal Title
Natural Product Communications
Volume: 9
Pages: 351-354
Related Report
Peer Reviewed
-
[Journal Article] Seed Dormancy Breaking Diterpenoids from the Liverwort Plagiochila sciophila and their Differentiation Inducing Activity in Human Acute Promyelocytic Leukemia HL-60 Cells.2014
Author(s)
Kenmoku, H., Tada, H., Oogushi, M., EsumiI, T., Takahashi, T., NojiI, M., Sassa, T., Toyota, M., and Asakawa, Y
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Journal Title
Natural Product Communications
Volume: 9
Related Report
Peer Reviewed
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