Molecular-based analysis of organ construction using proteomic analyis

Research Project

Project/Area Number	24651252
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Living organism molecular science
Research Institution	独立行政法人医薬基盤研究所
Principal Investigator	KAMADA Haruhiko 独立行政法人医薬基盤研究所, 創薬基盤研究部, サブプロジェクトリーダー (00324509)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	プロテオミクス
Research Abstract	In this study, we focused on protein expression pattern of CTOS membrane and extracellular matrix to discover the molecules related with the construction of 3D organ structure. Moreover, we created new method to culture CTOS as flat culture. Several proteins were identified as specific expression in spheroid CTOS but not flat –cultured CTOS expressed in membrane and extracellular matrix, which estimate to reflect heavily culture patterns, using proteomic analysis with biotinylation. These proteins were suggested to have an important role to construct the organ structure and will be clarify the activity in 3D structure in future.

Report

(3 results)