Molecular-based analysis of organ construction using proteomic analyis
| Project/Area Number |
24651252
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | 独立行政法人医薬基盤研究所 |
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Principal Investigator |
KAMADA Haruhiko 独立行政法人医薬基盤研究所, 創薬基盤研究部, サブプロジェクトリーダー (00324509)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | プロテオミクス |
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| Research Abstract |
In this study, we focused on protein expression pattern of CTOS membrane and extracellular matrix to discover the molecules related with the construction of 3D organ structure. Moreover, we created new method to culture CTOS as flat culture. Several proteins were identified as specific expression in spheroid CTOS but not flat –cultured CTOS expressed in membrane and extracellular matrix, which estimate to reflect heavily culture patterns, using proteomic analysis with biotinylation. These proteins were suggested to have an important role to construct the organ structure and will be clarify the activity in 3D structure in future.
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Report
(3 results)
Research Products
(5 results)
