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Molecular-based analysis of organ construction using proteomic analyis

Research Project

Project/Area Number 24651252
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Living organism molecular science
Research Institution独立行政法人医薬基盤研究所

Principal Investigator

KAMADA Haruhiko  独立行政法人医薬基盤研究所, 創薬基盤研究部, サブプロジェクトリーダー (00324509)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsプロテオミクス
Research Abstract

In this study, we focused on protein expression pattern of CTOS membrane and extracellular matrix to discover the molecules related with the construction of 3D organ structure. Moreover, we created new method to culture CTOS as flat culture. Several proteins were identified as specific expression in spheroid CTOS but not flat –cultured CTOS expressed in membrane and extracellular matrix, which estimate to reflect heavily culture patterns, using proteomic analysis with biotinylation. These proteins were suggested to have an important role to construct the organ structure and will be clarify the activity in 3D structure in future.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (5 results)

All 2012 Other

All Presentation (4 results) Remarks (1 results)

  • [Presentation] Exosome由来膜タンパク質のプロテオーム解析による新規肺がんバイオマーカーの探索2012

    • Author(s)
      山下琢矢,鎌田春彦, 他
    • Organizer
      第37回日本医用マススペクトル学会年会
    • Place of Presentation
      名古屋(愛知)
    • Related Report
      2013 Final Research Report
  • [Presentation] がん細胞分泌エクソソームのプロテオーム解析によるバイオマーカー候補蛋白質の探索2012

    • Author(s)
      鎌田春彦, 他
    • Organizer
      日本プロテオーム機構第10回大会
    • Place of Presentation
      東京(東京)
    • Related Report
      2013 Final Research Report
  • [Presentation] Exosome由来膜タンパク質のプロテオーム解析による新規肺がんバイオマーカーの探索2012

    • Author(s)
      山下琢矢, 鎌田春彦 他
    • Organizer
      第37回日本医用マススペクトル学会年会
    • Place of Presentation
      名古屋(愛知)
    • Related Report
      2012 Research-status Report
  • [Presentation] がん細胞分泌エクソソームのプロテオーム解析によるバイオマーカー候補蛋白質の探索.2012

    • Author(s)
      鎌田 春彦 他
    • Organizer
      日本プロテオーム機構 第10回大会
    • Place of Presentation
      東京(東京)
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.nibio.go.jp/bio-r/index.htm

    • Related Report
      2013 Final Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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