| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
We have developed a red colour-emitting luminescent iridium complex probe BTP for detecting hypoxic region in vivo, and examined why reactive oxygen species ROS is more generated in a hypoxic condition than in a normoxic condition. We used primary-cultured rat cardiac muscle cells because their metabolic activity is easily controllable in a cellculture system. When the cells were stimulated by β-agonist, their beating movement increased in a dose-dependent manner, and they were turned hypoxic by BTO luminescent intensity. But this intensity was weakened with β-blocker.
Next, when we observed ROS generation with green colour-emitting fluorescent probe RT-TMROS, green colour was getting intensified with increase of BTP red color. But when active form of glutathione was added to the culture, green-colour was not augmented even in a hypoxic condition. We suggest that increased ROS generation in a hypoxic condition results from ROS-eliminating action by GSH-dependent peroxidase.
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