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Detection of antimicrobial peptide-based universal detection system of enzyme activities and its application to theranostics

Research Project

Project/Area Number 24651259
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Chemical biology
Research InstitutionOsaka University

Principal Investigator

MIZUKAMI Shin  大阪大学, 工学(系)研究科(研究院), 准教授 (30420433)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords抗菌ペプチド / 蛍光 / 酵素活性 / 酵素活性検出 / リポソーム / セラノスティクス
Outline of Final Research Achievements

So far, we had developed a light-responsive compound release system by designing a functional antimicrobial peptide, temporin L (TL), modified with a photo-responsive protective group and combining liposomes including fluorophores. In this study, we newly developed an enzyme-responsive compound release system by making fusion peptides of TL and enzyme substrates. We chose caspase-3 and phosphatases as the target enzymes. For caspase-3, the enzyme substrate peptide was modified to the ε-amino group to yield a branched peptide. For phosphatases, a phosphorylated amino acid was substituted with one of the amino acids in TL. In both cases, the target enzyme activity activated the membrane-damaging activity of the fusion peptides. By using liposome including fluorophores, the system was applied to fluorescence detection of the target enzymes.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (19 results)

All 2015 2014 2013 2012 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results,  Acknowledgement Compliant: 1 results,  Open Access: 2 results) Presentation (8 results) (of which Invited: 3 results) Remarks (1 results)

  • [Journal Article] Actavataqble 19F MRI nanoparticle probes for the detection of reducing environments2015

    • Author(s)
      Nkamura, T., Matsushita, H., Sugihara, F., Yoshioka, Y., Mizukami, S. and Kikuchi, K.
    • Journal Title

      Angewandte Chemi International Edition

      Volume: 54 Issue: 3 Pages: 1007-1010

    • DOI

      10.1002/anie.201409365

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mesoporous slilica nanoparticles for 19F magnetic resonance imaging, fluorescence imaging, and drug deliverry2015

    • Author(s)
      Nakamura, T., Sugihara, F., Matsushita, H., Yoshioka, Y., <izukami, S., and Kikuchi, K.
    • Journal Title

      Chemical Science

      Volume: 6 Issue: 3 Pages: 1986-1990

    • DOI

      10.1039/c4sc03549f

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ratiometric MRI Sensors Based on Core-Shell Nanoparticles for Quantitative pH Imaging2014

    • Author(s)
      Satoshi Okada, Shin Mizukami, Takao Sakata, Yutaka Matsumura, Yoshichika Yoshioka, Kazuya Kikuchi
    • Journal Title

      Adv. Mater.

      Volume: 26 Issue: 19 Pages: 2989-2992

    • DOI

      10.1002/adma.201305018

    • NAID

      40021302212

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Nanospherical Polymer as an MRI Sensor without Paramagnetic or Superparamagnetic Species2013

    • Author(s)
      Satoshi Okada, Shin Mizukami, Yutaka Matsumura, Yoshichika Yoshioka, Kazuya Kikuchi
    • Journal Title

      Dalton Trans.

      Volume: 42 Issue: 45 Pages: 15864-15867

    • DOI

      10.1039/c3dt50378j

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Efficient Development of Luminescent Lanthanide(III) Complexes by Solid-Phase Synthesis and On-Resin Screening2013

    • Author(s)
      Tatsuya Nakamura, Shin Mizukami, Miho Tanaka, Kazuya Kikuchi
    • Journal Title

      Chem. Asian J.

      Volume: 8 Issue: 11 Pages: 2685-2690

    • DOI

      10.1002/asia.201300759

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Development of Luminescent Coelenterazine Derivatives Activatable by β-Galactosidase for Monitoring Dual Gene Expression2013

    • Author(s)
      Lindberg E, Mizukami S, Ibata K, Miyawaki A, Kikuchi K
    • Journal Title

      Chem. Eur. J.

      Volume: 19 Issue: 44 Pages: 14970-14976

    • DOI

      10.1002/chem.201302002

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Development of Cell-Impermeable Coelenterazine Derivatives2013

    • Author(s)
      Lindberg E, Mizukami S, Ibata K, Fukano T, Miyawaki A, Kikuchi K
    • Journal Title

      Chem. Sci.

      Volume: 4 Issue: 12 Pages: 4395-4400

    • DOI

      10.1039/c3sc51985f

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Multifunctional Core-shell Silica Nanoparticles for Highly Sensitive ^<19>F MRI2013

    • Author(s)
      H. Matsushita, S. Mizukami, F. Sugihara, Y. Nakanishi, Y. Yoshioka & K. Kikuchi
    • Journal Title

      Angew. Chem. Int. Ed

      Volume: 52(in press) Issue: 4 Pages: 52-52

    • DOI

      10.1002/anie.201308500

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Small-Molecule-Based Protein-Labeling Technology in Live Cell Studies : Probe-Design Concepts and Applications2013

    • Author(s)
      S. Mizumkami, Y. Hori & K. Kikuchi
    • Journal Title

      Acc. Chem. Res

      Volume: 46(in press) Issue: 1 Pages: 247-256

    • DOI

      10.1021/ar400135f

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] ^<19>F MRI monitoring of Gene Expression in Living Cells via Cell Surface β-Lactamase Activity2012

    • Author(s)
      松下尚嗣、水上進、杉原文徳、白川昌宏、菊地和也
    • Journal Title

      ChemBioChem

      Volume: 13 Issue: 11 Pages: 1579-1583

    • DOI

      10.1002/cbic.201200331

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Development of smart 19F MRI probes for in vivo imaging2014

    • Author(s)
      Shin Mizukami
    • Organizer
      China/Japan Young Chemist Forum
    • Place of Presentation
      北京(中国)
    • Year and Date
      2014-08-05
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 機能性抗菌ペプチドと リポソームによる酵素活性検出法2013

    • Author(s)
      樫部 順美、水上進、菊地和也
    • Organizer
      日本化学会第93春季年会
    • Place of Presentation
      草津
    • Related Report
      2012 Research-status Report
  • [Presentation] 酵素活性をトリガーとする薬物放出システムの開発2012

    • Author(s)
      樫部 順美、水上進、菊地和也
    • Organizer
      第28回日本DDS学会
    • Place of Presentation
      札幌
    • Related Report
      2012 Research-status Report
  • [Presentation] リポソームと機能性ペプチドを用いた酵素活性検出法の開発2012

    • Author(s)
      樫部 順美、水上進、菊地和也
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Research-status Report
  • [Presentation] 抗菌ペプチドの機能化による酵素活性応答薬物放出法の開発

    • Author(s)
      樫部順美、 水上進、 菊地和也
    • Organizer
      第7回バイオ関連化学シンポジウム
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 機能化抗菌ペプチドを利用した刺激応答性化合物放出システムの開発

    • Author(s)
      樫部順美、 水上進、 菊地和也
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Research-status Report
  • [Presentation] ナノバイオプローブ設計を基盤とする 分子イメージング技術開発

    • Author(s)
      水上進
    • Organizer
      日本化学会 生体機能関連化学部会 若手の会 サマースクール
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 蛋白質機能を可視化する分子の設計と開発

    • Author(s)
      水上進
    • Organizer
      新学術領域研究 若手合同シンポジウム「配位プログラム」×「融合マテリアル」
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
    • Invited
  • [Remarks]

    • URL

      http://www-molpro.mls.eng.osaka-u.ac.jp/

    • Related Report
      2014 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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