| Project/Area Number |
24651259
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Osaka University |
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Principal Investigator |
MIZUKAMI Shin 大阪大学, 工学(系)研究科(研究院), 准教授 (30420433)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 抗菌ペプチド / 蛍光 / 酵素活性 / 酵素活性検出 / リポソーム / セラノスティクス |
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| Outline of Final Research Achievements |
So far, we had developed a light-responsive compound release system by designing a functional antimicrobial peptide, temporin L (TL), modified with a photo-responsive protective group and combining liposomes including fluorophores. In this study, we newly developed an enzyme-responsive compound release system by making fusion peptides of TL and enzyme substrates. We chose caspase-3 and phosphatases as the target enzymes. For caspase-3, the enzyme substrate peptide was modified to the ε-amino group to yield a branched peptide. For phosphatases, a phosphorylated amino acid was substituted with one of the amino acids in TL. In both cases, the target enzyme activity activated the membrane-damaging activity of the fusion peptides. By using liposome including fluorophores, the system was applied to fluorescence detection of the target enzymes.
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