| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Research Abstract |
Gram-negative bacteria produce outer membrane vesicles (OMVs), and OMVs have proposed roles in virulence, inflammation, and the response to envelope stress. We studied human antimicrobial effects against E. coli to evaluate whether OMVs-mediated antimicrobial protection occurs. Human antimicrobial peptides, human b-defensin (hBD-2) showed antmicrobial effects against E.coli by radial diffusion assay, and supplimentation with purified OMVs provided protection of E.coli against antimicrobial effects of hBD-2. To examine direct interaction of OMVs to hBD-2, fluorescence correlation spectroscopy (FCS) was used for this study with fluorescence-labeled hBD-2. By FCS, direct interaction OMVs to hBD-2 was detected. These findings offer direct binding bacterial OMVs to hBD-2 neutralized its antimicrobial activity.
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