Outer membrane vesicles (OMVs)mediated protection of E. coli against effects of human antimicrobial peptides.
| Project/Area Number |
24651263
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Nagasaki University |
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Principal Investigator |
WADA Akihiro 長崎大学, 熱帯医学研究所, 講師 (70253698)
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| Project Period (FY) |
2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | ディフェンシン / 抗菌性ペプチド / アウターメンブランベシクル / OMV |
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| Research Abstract |
Gram-negative bacteria produce outer membrane vesicles (OMVs), and OMVs have proposed roles in virulence, inflammation, and the response to envelope stress. We studied human antimicrobial effects against E. coli to evaluate whether OMVs-mediated antimicrobial protection occurs. Human antimicrobial peptides, human b-defensin (hBD-2) showed antmicrobial effects against E.coli by radial diffusion assay, and supplimentation with purified OMVs provided protection of E.coli against antimicrobial effects of hBD-2. To examine direct interaction of OMVs to hBD-2, fluorescence correlation spectroscopy (FCS) was used for this study with fluorescence-labeled hBD-2. By FCS, direct interaction OMVs to hBD-2 was detected. These findings offer direct binding bacterial OMVs to hBD-2 neutralized its antimicrobial activity.
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Report
(2 results)
Research Products
(4 results)
