Development of a science-educational program for experience of biological diversity and evolution at the DNA level using plant species
| Project/Area Number |
24653282
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Education on school subjects and activities
|
|---|
| Research Institution | Kanagawa University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
|---|
| Keywords | 生物多様性 / 植物 / DNA / ポリメラーゼ連鎖反応法 / 制限酵素切断断片長分析 / 粗抽出物 / 電気泳動 |
|---|
| Outline of Final Research Achievements |
A comprehensive understanding of biodiversity is considered important when considering global environmental conservation. However, genetic diversity is less well known. We developed a simple practical program called ‘Kitchen PCR’ to communicate the concept of genetic diversity at the DNA level. Kitchen PCR essentially consists of three steps: Crude DNA extracts were prepared by homogenizing the plant materials in a Tris buffer, and the psbA-trnH intergenic region of the plastid genome was amplified by PCR. The amplified DNA fragments were fractionated by agarose gel electrophoresis and visualized by staining with ethidium bromide or Fast Blast DNA stain. Huge diversity the amplified DNA fragment lengths were clearly observed in the experimental plants. Kitchen PCR provides a simple method for demonstrating the concepts of genetic diversity at the DNA level and for introducing students to molecular biological techniques.
|
Report
(4 results)
Research Products
(6 results)
