| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
A comprehensive understanding of biodiversity is considered important when considering global environmental conservation. However, genetic diversity is less well known. We developed a simple practical program called ‘Kitchen PCR’ to communicate the concept of genetic diversity at the DNA level. Kitchen PCR essentially consists of three steps: Crude DNA extracts were prepared by homogenizing the plant materials in a Tris buffer, and the psbA-trnH intergenic region of the plastid genome was amplified by PCR. The amplified DNA fragments were fractionated by agarose gel electrophoresis and visualized by staining with ethidium bromide or Fast Blast DNA stain. Huge diversity the amplified DNA fragment lengths were clearly observed in the experimental plants. Kitchen PCR provides a simple method for demonstrating the concepts of genetic diversity at the DNA level and for introducing students to molecular biological techniques.
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