Study of effect of atmospheric pressure plasma on biological specimen by using in situ real time electron spin resonance technique

• Project/Area Number: 24654191
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Plasma science
• Research Institution: Nagoya University
• Principal Investigator: ISHIKAWA Kenji 名古屋大学, 工学(系)研究科(研究院), 教授 (60417384)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: プラズマ滅菌 / 電子スピン共鳴 / フリーラジカル / ミドリカビ胞子 / プラズマ殺菌

Research Abstract

Atmospheric pressure plasma sterilization of pathogens is now paid much attention for usefulness of no chemicals, electrical operations and so on. The plasma generates many kinds of reactive chemical species such as atomic oxygen, excited oxygen, hydroxyl radical, excited nitrogen, nitrous oxide, etc. However, interactions these species with biological specimens such as fungal spores, microorganisms etc. have not been clarified completely. In particular of intentional exposure of the reactive species to the biological specimen, the author has focused on understanding of free-radicals during plasma sterilization. Employing in situ real time electron spin resonance (ESR) technique, observation of free radical on the fungal spore of Penicillium digitatum has been succeeded. ESR signal decay is linked with inactivation of the spore, therefore the author found the close correlation of the free radical detected by ESR technique with biological response of inactivation on the fungal spores.

Research Products

• [Journal Article] Oxidation mechanism of Penicillium digitatum spores through neutral oxygen radicals (2014)
  • Author(s): H. Hashizume, T. Ohta, K. Takeda, K. Ishikawa, M. Hori, M. Ito
  • Journal Title: Japanese Journal of Applied Physics Volume: 53 Issue: 1 Pages: 10209-10209
  • DOI: 10.7567/jjap.53.010209
  • Peer Reviewed
• [Journal Article] Inactivation effects of neutral reactive-oxygen species on Penicillium digitatum spores using non-equilibrium atmospheric-pressure oxygen radical source (2013)
  • Author(s): H. Hashizume, T. Ohta, J. Fengdong, K. Takeda, K. Ishikawa, M. Hori, M. Ito
  • Journal Title: Applied Physics Letters Volume: 103 Issue: 15 Pages: 153708-153708
  • DOI: 10.1063/1.4824892
  • Peer Reviewed
• [Journal Article] Real-time In Situ Electron Spin Resonance Measurements on Fungal Spores of Penicillium digitatum during Exposure of Oxygen Plasmas (2012)
  • Author(s): Kenji Ishikawa, et al.
  • Journal Title: Appl. Phys. Lett Volume: 101 (1) Issue: 1 Pages: 13704-13704
  • DOI: 10.1063/1.4733387
  • Peer Reviewed
• [Journal Article] Electron Spin Resonance (ESR) Observation of Radicals on Biological Organism Interacted with Plasmas (2012)
  • Author(s): Kenji Ishikawa, Hiroko Moriyama, Kazuhiro Tamiya, Hiroshi Hashizume, Takayuki Ohta, Masafumi Ito, Sachiko Iseki, Hiromasa Tanaka, Keigo Takeda, Hiroki Kondo, Makoto Sekine and Masaru Hori
  • Journal Title: MRS Online Proceedings Library Volume: 1469
  • DOI: 10.1557/opl.2012.928
  • Peer Reviewed
• [Presentation] Electron spin resonance analyses of plasma-biological material interactions in atmospheric pressure plasmas (2014)
  • Author(s): Kenji Ishikawa, et al
  • Organizer: International workshop on control of fluctuation of plasma processes - Joint International Workshop between "Frontier science of interactions between plasmas and nano-interfaces" and "Plasma medical innovation"
  • Place of Presentation: 福岡(3B-WS-07)
• [Presentation] Electron spin resonance analyses of plasma-biological material interactions in atmospheric pressure plasmas (2014)
  • Author(s): Kenji Ishikawa
  • Organizer: International workshop on control of fluctuation of plasma processes - Joint International Workshop
  • Place of Presentation: Fukuoka Japan
  • Invited
• [Presentation] Chemical analyses of edible meat irradiated atmospherics-pressure-plasmas (2014)
  • Author(s): Kenji Ishikawa, Hiroshi Hashizume, Takayuki Ohta, Masafumi Ito, Hiromasa Tanaka, Keigo Takeda, Satomi Tajima, Hiroki Kondo, Makoto Sekine, and Masaru Hori
  • Organizer: The 8th International Conference on Reactive Plasmas (ICRP-8) at Fukuoka, February 2-6, 2014 (Fukuoka, Japan)
  • Place of Presentation: Fukuoka Japan
• [Presentation] Electron spin resonance study of radical generation during non-thermal plasma blood coagulation (2014)
  • Author(s): Kenji Ishikawa, Hiroshi Hashizume, Takayuki Ohta, Masafumi Ito, Hiromasa Tanaka, Keigo Takeda, Satomi Tajima, Hiroki Kondo, Makoto Sekine, and Masaru Hori
  • Organizer: 17th Biennial Meeting of Society for Free Radical Research International.
  • Place of Presentation: Kyoto Japan
• [Presentation] In-situ ESR measurements for plasma materials interactions (2013)
  • Author(s): Kenji Ishikawa, et al
  • Organizer: The 9th Asian-European International Conference on Plasma Surface Engineering (AEPSE)
  • Place of Presentation: 韓国(23-1)
• [Presentation] Electron spin resonance study of plasma-biological surface interaction for food hygiene (2013)
  • Author(s): Kenji Ishikawa, et al
  • Organizer: The 5th International Symposium on Advanced Plasma Science and its applications for nitrides and nanomaterials (ISPlasma)
  • Place of Presentation: 名古屋(P3025A)
• [Presentation] In-situ ESR measurements for plasma materials interactions (2013)
  • Author(s): Kenji Ishikawa
  • Organizer: 9th Asian-European International Conference on Plasma Surface Engineering
  • Place of Presentation: Jeju, Korea
  • Invited
• [Presentation] ミドリカビ胞子からの実時間その場ESR信号観察 (2013)
  • Author(s): 石川 健治，水野 寛子，田中 宏昌，橋爪 博司，太田 貴之, 伊藤 昌文，竹田 圭吾，近藤 博基，関根 誠，堀 勝
  • Organizer: 第66回日本酸化ストレス学会学術集会
  • Place of Presentation: 名古屋 日本
• [Presentation] Chemical analyses of edible meat irradiated atmospheric-pressure-plasmas (2013)
  • Author(s): Kenji Ishikawa, Hiroko Mizuno, Hiroshi Hashizume, Takayuki Ohta, Masafumi Ito, Hiromasa Tanaka, Keigo Takeda, Satomi Tajima, Hiroki Kondo, Makoto Sekine and Masaru Hori
  • Organizer: 2013 JSAP-MRS Joint Symposia
  • Place of Presentation: Kyoro Japan
• [Presentation] プラズマ－表面相互作用の実時間その場電子スピン共鳴分光研究 (2013)
  • Author(s): 石川健治，鷲見直也，河野昭彦，堀邊英夫，竹田圭吾，近藤博基，関根誠，堀勝
  • Organizer: 第74回応用物理学会秋季学術講演会
  • Place of Presentation: 京都 日本
  • Invited
• [Presentation] Electron spin resonance study of plasma-biological surface interaction for food hygiene (2013)
  • Author(s): Kenji Ishikawa, H. Mizuno, H. Tanaka, H. Hashizume, T. Ohta, M. Ito, K. Takeda, H. Kondo, M. Sekine, M. Hori
  • Organizer: The 5th International Symposium on Advanced Plasma Science and its applications for nitrides and nanomaterials (ISPlasma), P3025A
  • Place of Presentation: Nagoya, Japan
• [Presentation] Plasma-biological surface interaction investigated by electron spin resonance (2013)
  • Author(s): H. Mizuno, Kenji Ishikawa, H. Tanaka, H. Hashizume, T. Ohta, M. Ito, K. Takeda, H. Kondo, M. Sekine, M. Hori
  • Organizer: The 5th International Symposium on Advanced Plasma Science and its applications for nitrides and nanomaterials (ISPlasma), P3027A
  • Place of Presentation: Nagoya, Japan
• [Presentation] Identification of ESR signals arisen from Penicillium digitatum spores-3 (2013)
  • Author(s): Hiroko Mizuno, Kenji Ishikawa, Hiromasa Tanaka, Hiroshi Hashizume, Takayuki Ohta, Masafumi Ito, Keigo Takeda, Hiroki Kondo, Makoto Sekine, Masaru Hori
  • Organizer: 第60回応用物理学会春季学術講演会
  • Place of Presentation: 神奈川県, Japan
• [Presentation] Electron Spin Resonance (ESR) Observation of Radicals on Biological Organism Interacted with Plasmas (2012)
  • Author(s): Kenji Ishikawa et al.
  • Organizer: 2012 Spring Meeting of the Material Research Society, WW2.6
  • Place of Presentation: San Francisco, USA
• [Presentation] Electron Spin Resonance (ESR) study of Radicals on Biological Organism Created by Interaction with Plasma (2012)
  • Author(s): Kenji Ishikawa et al.
  • Organizer: International Conference on Plasma Science (ICOPS 2012), 2P-187.
  • Place of Presentation: Edinburgh, UK
• [Presentation] Real-time / In-situ Electron Spin Resonance Analysis of Surface Reactions on Organic / Biological Materials Interacted with Plasmas (2012)
  • Author(s): Kenji Ishikawa et al.
  • Organizer: International Univon of Materials Research Societies (IUMRS), International Conference on Electronic Materials(ICEM), C-8-I26-010
  • Place of Presentation: Yokohama, Japan
  • Invited
• [Presentation] Real-Time Electron Spin Resonance Study on Fungal Spores during Inactivation (2012)
  • Author(s): Kenji Ishikawa et al.
  • Organizer: APCPST 25th SPSM, 3P-04
  • Place of Presentation: Kyoto Japan
• [Presentation] Real time In Situ Electron Spin Resonance (ESR) Study of Free Radicals on Materials Created by Plasmas (2012)
  • Author(s): Kenji Ishikawa et al.
  • Organizer: American Vacuum Society (AVS), PS2-TuA7
  • Place of Presentation: Florida, USA
  • Invited
• [Presentation] Plasma-Biological Surface Interaction for Food Hygiene: Real-time in situ electron spin resonance measurements (2012)
  • Author(s): Kenji Ishikawa, H. Mizuno, H. Tanaka, H. Hashizume, T. Ohta, M. Ito, K. Takeda, H. Kondo, M. Sekine, and M. Hori
  • Organizer: The 34th International Symposium on Dry Process (DPS), B-3.
  • Place of Presentation: Tokyo, Japan
• [Presentation] Identification of ESR signals arisen from Penicillium digitatum spores-2 (2012)
  • Author(s): Hiroko Mizuno，Hiroshi Hashizume，Hiromasa Tanaka，Kenji Ishikawa，Takayuki Ohta，Masafumi Ito，Keigo Takeda，Hiroki Kondo，Makoto Sekine，Masaru Hori
  • Organizer: 2012年秋季 第73回応用物理学会学術講演会
  • Place of Presentation: 愛媛県, Japan
• [Remarks]
  • URL: http://www.plasma.engg.nagoya-u.ac.jp/ishikawa/index.php/Home
• [Patent(Industrial Property Rights)] 滅菌表示装置および滅菌装置および青果物表皮の殺菌方法 (2012)
  • Inventor(s): 石川健治、堀勝、伊藤昌文、太田貴之、橋爪博司
  • Industrial Property Rights Holder: 名古屋大学、名城大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-02-23