| Project/Area Number |
24655006
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical chemistry
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
SAKAI Makoto 東京工業大学, 資源化学研究所, 准教授 (60298172)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 水溶液 / 赤外分光 / 生体分子 / 超解像 / ローダミン６G / 超解像顕微鏡 |
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| Outline of Final Research Achievements |
Water intensely absorbs IR light. Therefore, IR light can penetrate only up to a few tens of micrometers and it is impossible to observe the IR absorption spectrum of solute molecules in the water by traditional IR spectroscopy. In this project, we applied a new detection system based on the TFD-IR microscopic technique. In this case, the visible and IR lasers are focused by an objective reflection lens and the transient fluorescence from the solute molecule in the water is collected by the same objective. Only the transient fluorescence near the focal point of the objective is observed, and we can monitor the IR absorption process of solute molecules located near the surface where a sufficient amount of IR light can penetrate even in the strongly IR absorbing solution. Using this system, we succeeded in monitoring TFD-IR signal of rhodamine 6G molecules in the water, and observing the TFD-IR spectrum that is corresponding to IR absorption spectrum in the 1100-1800 cm-1 region.
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