Novel protein reconstitution systems for monitoring protein dynamics in living cells

• Project/Area Number: 24655058
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Analytical chemistry
• Research Institution: University of Toyama (2013); The University of Tokyo (2012)
• Principal Investigator: KANNO Akira 富山大学, 大学院理工学研究部(工学), 助教 (60466795)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 分析化学 / 生体分析

Research Abstract

In this study, I developed two types of indicators to monitor the dynamics of biomolecules in living cells: luciferase fragments fused to a fluorescent protein for the highly precise detection of phosphatidylinositol 3,4,5-trisphosphate (PIP3) production in living cells; and cyclic luciferases for real-time sensing of caspase-3 and -8 in living cells with high sensitivity.

Research Products

• [Journal Article] Bioluminescent Probes to Analyze Ligand-induced Phosphatidylinositol 3,4,5-Trisphosphate Production with Split Luciferase Complementation (2013)
  • Author(s): L.Z. Yang, Y. Nasu, M. Hattori, H. Yoshimura, A. Kanno, and T. Ozawa
  • Journal Title: Anal. Chem. Volume: 85 Pages: 11352-11359
• [Journal Article] Bioluminescent Probes to Analyze Ligand-Induced Phosphatidylinositol 3,4,5-Trisphosphate Production with Split Luciferase Complementation (2013)
  • Author(s): Lingzhi Yang, Yusuke Nasu, Mitsuru Hattori, Hideaki Yoshimura, Akira Kanno, and Takeaki Ozawa
  • Journal Title: Analytical Chemistry Volume: 85
• [Presentation] エストロゲンを高感度にリアルタイム検出する発光インジケーターの開発 (2012)
  • Author(s): 菅野憲,竹之内修,高倉栄男,小澤岳昌
  • Organizer: 第72回分析化学討論会
  • Place of Presentation: 鹿児島(口頭発表)
• [Presentation] エストロゲンを高感度にリアルタイム検出する発光インジケーターの開発 (2012)
  • Author(s): ○菅野憲，竹之内修，高倉栄男，小澤岳昌
  • Organizer: 第72回分析化学討論会
  • Place of Presentation: 鹿児島
• [Book] 発光の事典 (2014)
  • Author(s): 菅野憲，小澤岳昌（木下修一ほか編）
  • Publisher: 朝倉書店
• [Book] 発光の事典
  • Author(s): 菅野憲,小澤岳昌(木下修一ほか編)
  • Publisher: 朝倉書店(in press)