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Development of new stain method for transmission electron microscope and demonstration of the usefulness

Research Project

Project/Area Number 24655101
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Polymer chemistry
Research InstitutionShinshu University

Principal Investigator

HANABUSA Kenji  信州大学, 総合工学系研究科, 教授 (60126696)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywords透過型電子顕微鏡 / 染色法 / ポリシロキサン / ポリジメチルシロキサン / チタン含有ポリマー
Research Abstract

The purpose of this study was to prove the usefulness of polysiloxanes as a new stain for transmission electron microscope. Thus far osmium (VIII) oxide, which is very expensive and extremely harmful, has been used for the stain in transmission electron microscope observation. We proved that polysiloxanes have useful potential as stain instead of osmium (VIII) oxide. Polysiloxanes are characterized of cheap, commercial available, and innoxious. Polysiloxane having high molecular weight is not necessarily for effective staining. In fact, low molecular weight siloxanes having high boiling point and oligosiloxanes also show effective staining for transmission electron microscope.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (10 results)

All 2014 2013 Other

All Journal Article (4 results) (of which Peer Reviewed: 2 results) Presentation (5 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] オイルゲル化剤に関する最近の動向2014

    • Author(s)
      英謙二
    • Journal Title

      高分子

      Volume: 63巻 Pages: 180-184

    • Related Report
      2013 Final Research Report
  • [Journal Article] Thixotropic Hydrogelators Based on a Cyclo(dipeptide) Derivative2013

    • Author(s)
      H. Hoshizawa, Y. Minemura, K. Yoshikawa, M. Suzuki, K. Hanabusa
    • Journal Title

      Langumir

      Volume: 29 Pages: 14666-14673

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] オイルゲル化剤の最近の展望と応用2013

    • Author(s)
      英謙二
    • Journal Title

      オレオサイエンス

      Volume: 13巻 Pages: 67-72

    • Related Report
      2013 Final Research Report
  • [Journal Article] Thixotropic hydrogelators based on a cyclo(dipeptide) derivative2013

    • Author(s)
      H. Hoshizawa, Y. Minemura, K. Yoshikawa, M. Suzuki, K. Hanabusa
    • Journal Title

      Langmuir

      Volume: 29 Issue: 47 Pages: 14666-14673

    • DOI

      10.1021/la402333h

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 低分子化合物によるゲル化機構の解明とゲル化剤の開発に関する研究2013

    • Author(s)
      英謙二
    • Organizer
      日本学術会議中部地区会議学術講演会
    • Place of Presentation
      信州大学理学部
    • Year and Date
      2013-07-17
    • Related Report
      2013 Final Research Report
  • [Presentation] ポリシロキサンを用いた新規染色法によるゲルのTEM観察2013

    • Author(s)
      英謙二・中島正志・池田義隆・鈴木正浩
    • Organizer
      平成25年度繊維学会年次大会予稿集
    • Place of Presentation
      2F10
    • Related Report
      2013 Final Research Report
  • [Presentation] 低分子化合物によるゲル化機構の解明とゲル化剤の開発に関する研究2013

    • Author(s)
      英謙二
    • Organizer
      日本学術会議中部地区会議学術講演会
    • Place of Presentation
      信州大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ポリシロキサンを用いた新規染色法によるゲルのTEM観察2013

    • Author(s)
      英 謙二・中島正志・池田義隆・鈴木正浩
    • Organizer
      平成25年度繊維学会年次大会
    • Place of Presentation
      東京タワーホール船堀
    • Related Report
      2013 Annual Research Report
  • [Presentation] ポリシロキサンを用いた新規染色法によるゲルのTEM観察2013

    • Author(s)
      英謙二、中島正志、池田義隆、鈴木正浩
    • Organizer
      平成25年度繊維学会年次大会
    • Place of Presentation
      タワーホール船堀
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://fiber.shinshu-u.ac.jp/hanabusasuzuki/index.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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