Development of structured PEG for protein manipulation

• Project/Area Number: 24655112
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Functional materials chemistry
• Research Institution: Tohoku University
• Principal Investigator: MURAOKA Takahiro 東北大学, 多元物質科学研究所, 助教 (70509132)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ポリエチレングリコール / ペンタエリスリトール / 凝集抑制効果 / タンパク質

Research Abstract

Influences of topology and amphiphilicity of poly(ethylene glycol) were studied. Triangle poly(ethylene glycol) derivative was synthesized by combining tetra ethylene glycol and pentaerythritol, which showed lower dehydration temperature than the linear counterpart. Furthermore, it was found that the triangle poly(ethylene glycol) has protein stabilization effect. Phenyl-appended poly(ethylene glycol)s were also synthesized and the difference of the poly(ethylene glycol) chain affected the assembling property in water.

Research Products

• [Journal Article] Thermoresponsive Self-assembly and Conformational Changes of Amphiphilic Monodisperse Short Poly(ethylene glycol)s in Water Nabanita Sadhukhan (2014)
  • Author(s): Takahiro Muraoka, Daisuke Abe, Yuji Sasanuma, Dwiky Rendra Graha Subekti and Kazushi Kinbara
  • Journal Title: Chem. Lett. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Transetherification on Polyols by Intra- and Intermolecular Nucleophilic Substitutions (2014)
  • Author(s): Takahiro Muraoka, Kota Adachi, Rainy Chowdhury and Kazushi Kinbara
  • Journal Title: PLoS ONE Volume: 9
  • Peer Reviewed
• [Journal Article] Thermal-Aggregation Suppression of Proteins by a Structured PEG Analogue : Importance of Denaturation Temperature for Effective Aggregation Suppression (2014)
  • Author(s): Takahiro Muraoka, Nabanita Sadhukhan, Mihoko Ui, Shunichi Kawasaki, Enrikko Hazemi, Kota Adachi, and Kazushi Kinbara
  • Journal Title: Biochem. Eng. J. Volume: 86C
  • Peer Reviewed
• [Journal Article] Thermoresponsive Self-assembly and Conformational Changes of Amphiphilic Monodisperse Short Poly(ethylene glycol)s in Water (2014)
  • Author(s): Nabanita Sadhukhan, Takahiro Muraoka, Daisuke Abe, Yuji Sasanuma, Dwiky Rendra Graha Subekti and Kazushi Kinbara
  • Journal Title: Chemistry Letters Volume: 43
  • NAID: 130004868253
  • Peer Reviewed
• [Journal Article] Thermal-Aggregation Suppression of Proteins by a Structured PEG Analogue : Importance of Denaturation Temperature for Effective Aggregation Suppression (2014)
  • Author(s): Takahiro Muraoka, Nabanita Sadhukhan, Mihoko Ui, Shunichi Kawasaki, Enrikko Hazemi, Kota Adachi, and Kazushi Kinbara
  • Journal Title: Biochem. Eng. J Volume: 86C Pages: 41-48
  • DOI: 10.1016/j.bej.2014.03.001
  • Peer Reviewed
• [Journal Article] Development and Functionalization of Structural Mimics of Multipass Transmembrane Proteins (2013)
  • Author(s): Takahiro Muraoka and Kazushi Kinbara
  • Journal Title: J. Syn. Org. Chem. Jpn. Volume: 71
  • NAID: 10031203356
  • Peer Reviewed
• [Journal Article] A Structured Monodisperse PEG for the Effective Suppression of Protein Aggregation (2013)
  • Author(s): Takahiro Muraoka, Kota Adachi, Mihoko Ui, Shunichi Kawasaki, Nabanita Sadhukhan, Haruki Obara, Hidehito Tochio, Masahiro Shirakawa, and Kazushi Kinbara
  • Journal Title: Angew. Chem., Int. Ed. Volume: 52
  • Peer Reviewed
• [Journal Article] Development and Functionalization of Structural Mimics of Multipass Transmembrane Proteins (2013)
  • Author(s): Takahiro Muraoka, Kazushi Kinbara
  • Journal Title: Journal of Synthetic Organic Chemistry, Japan Volume: 71 Issue: 10 Pages: 1045-1050
  • DOI: 10.5059/yukigoseikyokaishi.71.1045
  • NAID: 10031203356
  • ISSN: 0037-9980, 1883-6526
  • Peer Reviewed
• [Journal Article] A Structured Monodisperse PEG for the Effective Suppression of Protein Aggregation (2013)
  • Author(s): Takahiro Muraoka, Kota Adachi, Mihoko Ui, Shunichi Kawasaki, Nabanita Sadhukhan, Hamki Obara, Hidehito Tochio, Masahiro Shirakawa, and Kazushi Kinbara
  • Journal Title: Angew. Chem., Int. Ed Volume: 52 Issue: 9 Pages: 2430-2434
  • DOI: 10.1002/anie.201206563
  • Peer Reviewed
• [Journal Article] Ion Permeation by a Folded Multiblock Amphiphilic Oligomer Achieved by Hierarchical Construction of Self-Assembled Nanopores (2012)
  • Author(s): Takahiro Muraoka, Tatsuya Shima, Tsutomu Hamada, Masamune Morita, Masahiro Takagi, Kazuhito V. Tabata, Hiroyuki Noji, and Kazushi Kinbara
  • Journal Title: J. Am. Chem. Soc. Volume: 134
  • Peer Reviewed
• [Journal Article] Development of Photoresponsive Supramolecular Machines Inspired by Biological Molecular Systems (2012)
  • Author(s): Takahiro Muraoka, and Kazushi Kinbara
  • Journal Title: J. Photoch. Photobio. C Volume: 13
  • Peer Reviewed
• [Journal Article] Development of Photoresponsive Supramolecular Machines Inspired by Biological Molecular Systems (2012)
  • Author(s): T. Muraoka, et al.
  • Journal Title: Journal of Photochemistry and Photobiology, C Volume: 13 Issue: 2 Pages: 136-147
  • DOI: 10.1016/j.jphotochemrev.2012.04.001
  • Peer Reviewed
• [Presentation] 膜タンパク質模倣分子の開発と機能 (2014)
  • Author(s): 村岡貴博
  • Organizer: 野口遵研究助成金講演会
  • Place of Presentation: 東京(口頭発表)
  • Year and Date: 2014-03-11
  • Invited
• [Presentation] Development of Chiral Synthetic Transmembrane Protein Mimics (2013)
  • Author(s): Daiki Noguchi, Takahiro Muraoka, Kazushi Kinbara
  • Organizer: 12th International Conference on Frontiers of Polymers and Advanced Materials
  • Place of Presentation: ニュージーランド、Auckland(口頭発表)
  • Year and Date: 2013-12-09
• [Presentation] Non-linear amphiphilic discrete oligo(ethylene glycol)s : synthesis and protein modification (2013)
  • Author(s): Adam M. Wawro, Takahiro Muraoka, Mihoko Ui, Kazushi Kinbara
  • Organizer: 12th International Conference on Frontiers of Polymers and Advanced Materials
  • Place of Presentation: ニュージーランド、Auckland(口頭発表)
  • Year and Date: 2013-12-09
• [Presentation] Monodisperse Amphiphilic PEG Macromonomer Refolds Protein Effectively (2013)
  • Author(s): Nabanita Sadhukhan, Takahiro Muraoka, Mihoko Ui, Kazushi Kinbara
  • Organizer: XVI National Conference On Surfactants, Emulsions and Biocolloids (NATCOSEB)
  • Place of Presentation: インド、Chennai(口頭発表)
  • Year and Date: 2013-11-29
  • Invited
• [Presentation] Functionalized PEGs As An Effective Protein Manipulator (2013)
  • Author(s): Sadhukhan Nabanita, Muraoka Takahiro, Mihoko Ui, Kinbara Kazushi
  • Organizer: 日本化学会東北支部70周年記念国際会議
  • Place of Presentation: 仙台(ポスター発表)
  • Year and Date: 2013-09-30
• [Presentation] Development of PEG-Based Functional Molecules (2013)
  • Author(s): Hidetaka Honda, Takahiro Muraoka, Kazushi Kinbara
  • Organizer: 日本化学会東北支部70周年記念国際会議
  • Place of Presentation: 仙台(ポスター発表)
  • Year and Date: 2013-09-30
• [Presentation] Development of a Photoresponsive Hybrid Protein (2013)
  • Author(s): Saori Miura, Takahiro Muraoka, Mihoko Ui, Kazushi Kinbara
  • Organizer: 日本化学会東北支部70周年記念国際会議
  • Place of Presentation: 仙台(ポスター発表)
  • Year and Date: 2013-09-30
• [Presentation] Development of functional amphiphiles operative in aqueous media (2013)
  • Author(s): Takahiro Muraoka, Kazushi Kinbara
  • Organizer: 日本化学会東北支部70周年記念国際会議
  • Place of Presentation: 仙台(ポスター発表)
  • Year and Date: 2013-09-30
• [Presentation] Liquid Crystalline Property of Cyclic Multi–Block Amphiphiles (2013)
  • Author(s): Tatsuya Shima, Takahiro Muraoka, Kiyoshi Kanie, Atsushi Muramatsu, Kazushi Kinbara
  • Organizer: 日本化学会東北支部70周年記念国際会議
  • Place of Presentation: 仙台(ポスター発表)
  • Year and Date: 2013-09-30
• [Presentation] 膜貫通型機能性分子の開発と評価 (2013)
  • Author(s): 梅津かおり,村岡貴博
  • Organizer: 日本化学会東北支部70周年記念国際会議
  • Place of Presentation: 仙台(ポスター発表)
  • Year and Date: 2013-09-30
• [Presentation] 生体に使う、生体を真似る機能性分子開発 (2013)
  • Author(s): 村岡貴博
  • Organizer: 御茶ノ水女子大学化学科講演会
  • Place of Presentation: 東京(口頭発表)
  • Year and Date: 2013-09-02
  • Invited
• [Presentation] Discrete PEGs with Distinctive Functions (2013)
  • Author(s): 村岡貴博
  • Organizer: Collaborative Conference on Materials Research
  • Place of Presentation: 韓国,済州島(口頭発表)
  • Year and Date: 2013-06-24
  • Invited
• [Presentation] 水を場とする超分子化学の展開 (2013)
  • Author(s): 村岡貴博
  • Organizer: 多元研生物物理オープンセミナー
  • Place of Presentation: 仙台
  • Invited
• [Presentation] 生体に使う、生体を真似る機能性分子開発 (2013)
  • Author(s): 村岡貴博
  • Organizer: 御茶ノ水女子大学 化学科講演会
  • Place of Presentation: 東京
  • Invited
• [Presentation] マルチブロック両親媒性分子の液晶特性 (2013)
  • Author(s): 嶋建也, 村岡貴博, 蟹江澄志, 村松淳司, 金原数
  • Organizer: 第62回高分子学会年次大会
  • Place of Presentation: 京都
• [Presentation] 構造化PEGの開発と生体関連機能 (2013)
  • Author(s): 村岡貴博
  • Organizer: 第25回生体機能関連化学若手の会サマースクール
  • Place of Presentation: 東京
• [Presentation] Development of a Photoresponsive Hybrid Protein (2013)
  • Author(s): Saori Miura, Takahiro Muraoka, Mihoko Ui, Kazushi Kinbara
  • Organizer: 東北大学化学系サマースクール
  • Place of Presentation: 仙台
• [Presentation] リガンド応答型膜挿入分子の開発と機能 (2013)
  • Author(s): 村岡 貴博, 遠藤 貴宏, 田端 和仁, 野地 博行, 金原 数
  • Organizer: 第62回高分子討論会
  • Place of Presentation: 金沢
• [Presentation] 膜貫通型機能性分子の開発と評価 (2013)
  • Author(s): 梅津 かおり, 村岡 貴博,金原 数
  • Organizer: 第62回高分子討論会
  • Place of Presentation: 金沢
• [Presentation] キラリティーを持った膜貫通型分子の開発 (2013)
  • Author(s): 野口大樹, 村岡貴博, 金原 数
  • Organizer: 第3回CSJ化学フェスタ2013
  • Place of Presentation: 東京
• [Presentation] マルチブロック両親媒性化合物の開発と応用 (2013)
  • Author(s): 村岡貴博, 金原数
  • Organizer: 2013高分子学会東北支部研究発表会
  • Place of Presentation: 仙台
• [Presentation] Discrete PEGs with Distinctive Functions (2013)
  • Author(s): Takahiro Muraoka
  • Organizer: Collaborative Conference on Materials Research
  • Place of Presentation: 韓国,済州島
  • Invited
• [Presentation] Monodisperse Amphiphilic PEG Macromonomer Refolds Protein Effectively (2013)
  • Author(s): Nabanita Sadhukhan, Takahiro Muraoka, Mihoko Ui, Kazushi Kinbara
  • Organizer: XVI National Conference On Surfactants, Emulsions and Biocolloids (NATCOSEB)
  • Place of Presentation: India,Chennai
  • Invited
• [Presentation] Development of a Molecular Sensor to Visualize the Structure of a Target (2013)
  • Author(s): Hidetaka Honda, Takahiro Muraoka, Kazushi Kinbara
  • Organizer: 東北大学化学系サマースクール
  • Place of Presentation: 仙台
• [Presentation] Functionalized PEGs As An Effective Protein Manipulator (2013)
  • Author(s): Sadhukhan Nabanita, Muraoka Takahiro, Mihoko Ui, Kinbara Kazushi
  • Organizer: International Symposium for 70th Anniversary of the Tohoku Branch of the Chemical Society of Japan
  • Place of Presentation: 仙台
• [Presentation] Development of PEG-Based Functional Molecules (2013)
  • Author(s): Hidetaka Honda, Takahiro Muraoka, Kazushi Kinbara
  • Organizer: 日本化学会東北支部70周年記念国際会議
  • Place of Presentation: 仙台
• [Presentation] Development of a Photoresponsive Hybrid Protein (2013)
  • Author(s): Saori Miura, Takahiro Muraoka, Mihoko Ui, Kazushi Kinbara
  • Organizer: 日本化学会東北支部70周年記念国際会議
  • Place of Presentation: 仙台
• [Presentation] Development of functional amphiphiles operative in aqueous media (2013)
  • Author(s): Takahiro Muraoka, Kazushi Kinbara
  • Organizer: 日本化学会東北支部70周年記念国際会議
  • Place of Presentation: 仙台
• [Presentation] Liquid Crystalline Property of Cyclic Multi-Block Amphiphiles (2013)
  • Author(s): Tatsuya Shima, Takahiro Muraoka, Kiyoshi Kanie, Atsushi Muramatsu, Kazushi Kinbara
  • Organizer: 日本化学会東北支部70周年記念国際会議
  • Place of Presentation: 仙台
• [Presentation] Properties and applications of discrete PEG amphiphiles (2013)
  • Author(s): Shunichi Kawasaki, Takahiro Muraoka, Haruki Obara, Kazushi Kinbara
  • Organizer: 日本化学会東北支部70周年記念国際会議
  • Place of Presentation: 仙台
• [Presentation] 膜貫通型機能性分子の開発と評価 (2013)
  • Author(s): 梅津かおり, 村岡貴博, 金原数
  • Organizer: 日本化学会東北支部70周年記念国際会議
  • Place of Presentation: 仙台
• [Presentation] Development of Chiral Synthetic Transmembrane Protein Mimics (2013)
  • Author(s): Daiki Noguchi, Takahiro Muraoka, Kazushi Kinbara
  • Organizer: 12th International Conference on Frontiers of Polymers and Advanced Materials
  • Place of Presentation: ニュージーランド,Auckland
• [Presentation] Non-linear amphiphilic discrete oligo(ethylene glycol)s: synthesis and protein modification (2013)
  • Author(s): Adam M. Wawro, Takahiro Muraoka, Mihoko Ui, Kazushi Kinbara
  • Organizer: 12th International Conference on Frontiers of Polymers and Advanced Materials
  • Place of Presentation: ニュージーランド,Auckland
• [Presentation] 構造化PEG分子を用いたタンパク質操作 (2013)
  • Author(s): 村岡貴博・安達皓太・宇井美穂子・河崎俊一・杤尾豪人・白川昌宏・金原数
  • Organizer: 日本化学会春季年会
  • Place of Presentation: 滋賀
• [Presentation] Development of Molecular Library for Protein Manipulation (2012)
  • Author(s): Nabanita Sadhukhan, Takahiro Muraoka, Mihoko Ui, Kazushi Kinbara
  • Organizer: 第61回高分子学会年次大会
  • Place of Presentation: 横浜
• [Presentation] 構造化PEG分子の合成とその性質 (2012)
  • Author(s): 村岡貴博, 安達皓太, 宇井美穂子, 河崎俊一, 小原春樹, 杤尾豪人, 白川昌宏, 金原数
  • Organizer: 第61回高分子学会年次大会
  • Place of Presentation: 横浜
• [Presentation] 構造化PEG分子の合成とその性質 (2012)
  • Author(s): 河崎俊一, 村岡貴博, 金原数
  • Organizer: 第24回万有札幌シンポジウム
  • Place of Presentation: 札幌
• [Presentation] 両親媒性構造化PEG分子の合成と特性 (2012)
  • Author(s): 河崎俊一, 村岡貴博, 金原数
  • Organizer: 第6回バイオ関連化学合同シンポジウム
  • Place of Presentation: 札幌
• [Presentation] PEGの構造化による新機能の発現 (2012)
  • Author(s): 村岡貴博, 安達皓太, 宇井美穂子, 河崎俊一, Nabanita SADHUKHAN, 小原春樹, 杤尾豪人, 白川昌宏, 金原数
  • Organizer: 第61回高分子討論会
  • Place of Presentation: 名古屋
• [Presentation] Synthesis of PEG Derivatives and Their Application in Protein Stabilization (2012)
  • Author(s): Nabanita Sadhukhan, Takahiro Muraoka, Mihoko Ui, Kazushi Kinbara
  • Organizer: 第61回高分子討論会
  • Place of Presentation: 名古屋
• [Presentation] 光応答性交互両親媒性化合物のリン脂質中での機能 (2012)
  • Author(s): 嶋建也, 村岡貴博, 濱田勉, 森田雅宗, 福岡創, 佐川貴志, 石島秋彦, 高木昌宏, 金原数
  • Organizer: 第2回CSJ化学フェスタ2012
  • Place of Presentation: 東京
• [Presentation] 構造化PEG分子の開発 (2012)
  • Author(s): 本多秀隆, 村岡貴博, 金原数
  • Organizer: 第2回CSJ化学フェスタ2012
  • Place of Presentation: 東京
• [Presentation] 複数回膜貫通分子の機能解析 (2012)
  • Author(s): 梅津かおり, 村岡貴博, 金原数
  • Organizer: 第2回CSJ化学フェスタ2012
  • Place of Presentation: 東京
• [Presentation] 高次構造を有する両親媒性オリゴマーの開発と機能開拓 (2012)
  • Author(s): 村岡貴博, 嶋建也, 濱田勉, 森田雅宗, 高木昌宏, 田端和仁, 野地博行, 金原数
  • Organizer: 第12回東北大学多元物質科学研究所研究発表会
  • Place of Presentation: 仙台
• [Presentation] Conformational and functional studies of synthetic mimics of multipass transmembrane proteins (2012)
  • Author(s): Takahiro Muraoka, Tatsuya Shima, Tsutomu Hamada, Masamune Morita, Masahiro Takagi, Kazushi Kinbara
  • Organizer: 13th Tetrahedron Symposium 2012
  • Place of Presentation: オランダ、アムステルダム
• [Presentation] Designer PEG-Conjugates for Protein Stabilization (2012)
  • Author(s): Nabanita Sadhukhan, Takahiro Muraoka, Mihoko Ui, Kazushi Kinbara
  • Organizer: The second Asian Chemical Biology Conference ACBC2012
  • Place of Presentation: 沖縄
• [Presentation] Function of Photoresponsive Alternating Amphiphilic Compounds in Phospholipids (2012)
  • Author(s): Tatsuya Shima, Takahiro Muraoka, Tsutomu Hamada, Masamune Morita, Hajime Fukuoka, Takashi Sagawa, Akihiko Ishijima, Kouhei Tsumoto, Masahiro Takagi, Kazushi Kinbara
  • Organizer: 第24回IUPAC光化学国際シンポジウム
  • Place of Presentation: ポルトガル、コインブラ
• [Presentation] Development of Discrete Macrocyclic PEG Analogues (2012)
  • Author(s): Shunichi Kawasaki, Takahiro Muraoka, Kazushi Kinbara
  • Organizer: キャンパスアジアサマースクール
  • Place of Presentation: 仙台
• [Presentation] Development of Structuralized Oligoethylene Glycols with Geometrical Diversity (2012)
  • Author(s): Haruki Obara, Takahiro Muraoka, Kazushi Kinbara
  • Organizer: キャンパスアジアサマースクール
  • Place of Presentation: 仙台
• [Presentation] Development of Synthetic Transmembrane-Protein Mimics Bearing Chiral Units (2012)
  • Author(s): Takahiro Endo, Takahiro Muraoka, Kazushi Kinbara
  • Organizer: キャンパスアジアサマースクール
  • Place of Presentation: 仙台
• [Presentation] Development of Discrete Macrocyclic PEG Analogues (2012)
  • Author(s): Shunichi Kawasaki, Takahiro Muraoka, Kazushi Kinbara
  • Organizer: 第9回国際高分子会議
  • Place of Presentation: 神戸
• [Presentation] Development of Structuralized Oligoethylene Glycols with Geometrical Diversity (2012)
  • Author(s): Haruki Obara, Takahiro Muraoka, Kazushi Kinbara
  • Organizer: 第9回国際高分子会議
  • Place of Presentation: 神戸
• [Presentation] Development of Synthetic Transmembrane-Protein Mimics Bearing Chiral Units (2012)
  • Author(s): Takahiro Endo, Takahiro Muraoka, Kazushi Kinbara
  • Organizer: 第9回国際高分子会議
  • Place of Presentation: 神戸
• [Presentation] Discrete Non-Linear PEGs for Protein Manipulation (2012)
  • Author(s): T. Muraoka, K. Adachi, M. Ui, S. Kawasaki, N. Sadhukhan, H. Obara, H. Tochio, M. Shirakawa, K. Kinbara
  • Organizer: 第9回国際高分子会議
  • Place of Presentation: 神戸
• [Presentation] Function of Photoresponsive Alternating Amphiphilic Molecule in Phospohlipids (2012)
  • Author(s): Tatsuya Shima, Takahiro Muraoka, Tsutomu Hamada, Masamune Morita, Hajime Fukuoka, Takashi Sagawa, Akihiko Ishijima, Masahiro Takagi, Kazushi Kinbara
  • Organizer: 第9回国際高分子会議
  • Place of Presentation: 神戸
• [Presentation] Measurement of cellular response of single E. coli to the photoreleased serine (2012)
  • Author(s): Takashi Sagawa, Hajime Fukuoka , Yuichi Inoue , Hiroto Takahashi, Takahiro Muraoka , Kazushi Kinbara , Akihiko Isjijima
  • Organizer: 日本生物物理学会名古屋年会
  • Place of Presentation: 名古屋
• [Remarks]
  • URL: http://www.tagen.tohoku.ac.jp/labo/kinbara/index-j.html
• [Remarks] 多元研 業績DB 個人業績表示
  • URL: http://db.tagen.tohoku.ac.jp/php/db/view-personal.php?pserial=2929
• [Remarks] 東北大学多元物質科学研究所金原研究室
  • URL: http://www.tagen.tohoku.ac.jp/labo/kinbara/index-j.html